2013－2019年贵州省副猪嗜血杆菌分离株耐药性及其演变

doi:10.16431/j.cnki.1671-7236.2020.10.034

摘要/Abstract

摘要： 试验旨在揭示贵州省副猪嗜血杆菌（Haemophilus parasuis，Hps）的耐药性及其变化趋势，指导Glasser's病临床防控用药，减缓Hps耐药菌株出现。通过K-B纸片琼脂法，检测2013－2019年分离自贵州省9个地区517株Hps对15种常用抗菌药的耐药性，分析不同分离时期和分离地区的菌株的耐药性差异。结果显示，针对15种抗生素分别有14.36%~70.58%的分离菌株表现耐药，其中耐头孢噻呋菌株的比例最低，耐复方新诺明菌株的比例最高；与2013年相比，2019年分离的Hps菌株对13种抗生素的耐药率分别升高了4.52%~324.68%，其中耐头孢噻呋菌株比例上升速度最快，达324.68%，耐链霉素菌株比例上升最慢，仅4.52%，耐土霉素和四环素菌株的比例分别下降2.21%和8.63%，出现负增长。相对于凯里、毕节和兴义地区，安顺、遵义、贵阳地区流行的菌株对头孢噻呋、替米考星、多西环素等在全省范围内耐药菌株比例较低的药物耐药率相对较高，而对复方新诺明、阿莫西林、链霉素等在全省范围内耐药菌株比例较高的药物耐药率相对较低。这一结果说明，贵州省Hps菌株对15种常用药物的耐药性较为严重，且总体呈现上升趋势，不同地区分离株对部分抗菌药的耐药率呈现明显的地区差异性。

关键词: 贵州省; 副猪嗜血杆菌(Hps); 药物敏感性; 耐药性

Abstract: This study was aimed to reveal the trend of drug resistance about Haemophilus parasuis (Hps) in Guizhou province and guide clinical use of Glasser's disease then slow the emergence of drug-resistant Hps strains.K-B slip-agar diffusion method was used to test the 15 common antibiotics resistance of 517 Hps strains isolated from 9 districts in Guizhou province from 2013 to 2019.The drug resistance differences of strains isolated from different time and districts were analyzed.The results showed that 14.36% to 70.58% isolates indicated drug resistance to 15 antibiotics,the proportion of EFT resistant strains was the lowest and SXT resistant strains was the highest.Compared with 2013,the resistance rate of Hps strains isolated in 2019 to 13 antibiotics increased by 4.52% to 324.68%.Among them,the proportion of drug-resistant strains of 13 drugs increased which EFT resistant strains increased at the fastest rate to 324.68% while STR resistant strains slowest rate to only 4.52%.The rate of TET resistant strains showed negative growth which decreased by 2.21% and 8.63%,respectively.Compared with Kaili,Bijie and Xingyi of Guizhou,the strains isolated from Anshun,Zunyi and Guiyang districts showed higher drug resistance rate of EFT,MC and DO which with low resistance in Guizhou province,but lower of SXT,AML and STR which with high resistance in Guizhou province.The results indicated that the resistance of Hps strains to 15 commonly antibiotics in Guizhou province was serious with rising tendency and the resistance rate of certain strains showed obvious differences between different districts.

Key words: Guizhou province; Haemophilus parasuis (Hps); drug sensitivity; drug resistance

中图分类号:

S852.61⁺2

史开志, 杜春林, 王婧, 张静, 杨茂生, 李永明. 2013－2019年贵州省副猪嗜血杆菌分离株耐药性及其演变[J]. 中国畜牧兽医, 2020, 47(10): 3343-3350.

SHI Kaizhi, DU Chunlin, WANG Jing, ZHANG Jing, YANG Maosheng, LI Yongming. Drug Resistance and Evolution of Haemophilus parasuis Isolates from Guizhou Province During 2013 to 2019[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3343-3350.

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