L-瓜氨酸对游泳力竭小鼠生长性能及血清生化指标影响的研究

doi:10.16431/j.cnki.1671-7236.2020.04.008

摘要/Abstract

摘要： 试验通过在饮水中添加不同剂量的L-瓜氨酸（L-Cit），探究其对游泳力竭强应激后，小鼠的生长性能及血清生化指标的影响。选用36只平均体重为（14.58±0.77）g的断奶雌性昆明小鼠，随机分为3组，每组6个重复。对照组饮用自来水，试验Ⅰ组和Ⅱ组在饮水中分别添加0.5%和1.0%的L-Cit。试验期21 d，每3 d空腹称重后在（31±2）℃水桶中负重游泳至力竭，于试验第21天摘眼球采血。结果表明，与对照组相比，试验Ⅰ组、Ⅱ组在第6、9、12天时，雌鼠体重分别提高了12.88%（P < 0.05）、20.30%（P < 0.01）、7.99%（P < 0.05）和21.27%（P < 0.01）、24.47%（P < 0.01）、19.46%（P < 0.01）。第15天时试验Ⅱ组小鼠体重比对照组提高13.71%（P < 0.01）。与对照组相比，试验Ⅰ组小鼠血清中抗超氧阴离子自由基（ASAFR）活力提高了31.49%（P < 0.01）；试验Ⅱ组血清中谷胱甘肽过氧化物酶（GSH-Px）活性和抑制羟自由基（IHFR）能力分别提高54.35%（P < 0.01）和13.10%（P < 0.01）；与对照组和试验Ⅰ组相比，试验Ⅱ组小鼠血清白蛋白（ALB）水平分别提高13.16%（P < 0.01）和6.28%（P < 0.01）。综合试验结果，饮水中添加L-Cit可提高运动后小鼠的生长性能、抗氧化性能及血清总蛋白（TP）和白蛋白含量。综合比较，添加1.0% L-Cit（69.09 mg/d）效果更好。

关键词: L-瓜氨酸; 小鼠; 生长性能; 抗氧化指标

Abstract: Effects of adding different doses of L-citrulline (L-Cit) in drinking water on the growth performance and serum biochemical indexes of swimming exhausted Kunming mice was studied in this paper.36 weaned females with average weight of (14.58±0.77) g were selected and randomly divided into 3 groups with 6 replicates in each group.The mice in control group drank from tap water,and 0.5% and 1% of L-Cit were added in the drinking water for test groupⅠand Ⅱ,respectively.The trial lasted 21 d.Every 3 d,the mice were weighed in an empty stomach and then swam with weight loading in (31±2)℃ bucket until exhausted.Blood was collected from the eyeballs at day 21.The results showed that:Compared with control group,at the day 6,9 and 12,the body weights of the female mice in test groups Ⅰ and Ⅱ increased by 12.88% (P < 0.05),20.30% (P < 0.01),7.99% (P < 0.05) and 21.27% (P < 0.01),24.47% (P < 0.01),19.46% (P < 0.01) respectively.And it increased by 13.71% (P < 0.01) at day 15 in test group Ⅱ.The serum activity of anti superoxide anion free radical (ASAFR) increased by 31.49% (P < 0.01) in test group Ⅰ.The serum activity of GSH-Px and inhibit hydroxyl free radical (IHFR) ability were increased by 54.35%(P < 0.01)and 13.10%(P < 0.01)in test group Ⅱ,respectively.Compared with the control group and test groupⅠ,the serum levels of serum albumin (ALB)content were respectively increased by 13.16% (P < 0.01) and 6.28% (P < 0.01).In conclusion,Adding L-Cit in drinking water could improve the growth performance and antioxidant capacity,increase serum total protein (TP) and albumin contents in mice after exercise,and adding 0.1% L-Cit (69.09 mg/d) was better.

Key words: L-citrulline; mice; growth performance; antioxidant indexes

中图分类号:

S816.7

赵国栋, 李晓斌, 李婷婷, 陈晖, 马晨, 曾无霜, 臧长江. L-瓜氨酸对游泳力竭小鼠生长性能及血清生化指标影响的研究[J]. 中国畜牧兽医, 2020, 47(4): 1035-1040.

ZHAO Guodong, LI Xiaobin, LI Tingting, CHEN hui, MA Chen, ZENG Wushuang, ZANG Changjiang. Study of L-citrulline on Growth Performance of Swimming Exhausted and Serum Biochemical Effect of Mice[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1035-1040.

参考文献

[1] TIMOTHY A,DAVID P,JACQUELINE S,et al.L-citrulline supplementation:Impact on cardiometabolic health[J].Nutrients,2018,10(7):921-945.
[2] CURIS E,NICOLIS I,MOINARD C,et al.Almost all about citrulline in mammals[J].Amino Acids,2005,29(3):177-205.
[3] ROUGE C,ROBERT C D,ROBINS C,et al.Manipulation of citrulline availability in humans[J].American Journal of Physiology-Gastrointestinal and Liver Physiology,2007,293(5):1061-1067.
[4] MOINARD C,NICOLIS I,NEVEUX N,et al.Dose-ranging effects of citrulline administration on plasma amino acids and hormonal patterns in healthy subjects:The citrudose pharmacokinetic study[J].British Journal of Nutrition,2008,99(4):855-862.
[5] MARK A,MOYAD M A.Complementary&Alternative Medicine for Prostate and Urologic Health[M].New York:Springer Science Business Media,2014.
[6] OSOWSKA S,DUCHEMANN T,WALRAND S,et al.Citrulline modulates muscle protein metabolism in old malnourished rats[J].American Journal of Physiology Endocrinology&Metabolism,2006,291(3):582.
[7] JOURDAN M,NAIR K S,CARTER R E,et al.Citrulline stimulates muscle protein synthesis in the post-absorptive state in healthy people fed a low-protein diet——A pilot study[J].Clinical Nutrition,2015,34(3):449-456.
[8] 刘娟,路欣欣,孟慧.瓜氨酸的药理作用及生产方法的研究进展[J].药学实践杂志,2011,29(3):173-175. LIU J,LU X X,MEN H.Progress on pharmacological activities and production methods of citrulline[J].Journal of Pharmaceutical Practice,2011,29(3):173-175.(in Chinese)
[9] 孙召君.瓜氨酸添加水平对哺乳仔猪生长性能及相关理化指标的影响[D].保定:河北农业大学,2012. SUN Z J.Effect of L-citrulline on the growth performance and related physical and biochemical indicators in suckling piglets[D].Baoding:Hebei Agricultural University,2012.(in Chinese)
[10] LIU T H,WU C L,CHIANG C W,et al.No effect of short-term arginine supplementation on nitric oxide production,metabolism and performance in intermittent exercise in athletes[J].Journal of Nutritional Biochemistry,2009,20(6):462-468.
[11] SUREDA A,CORDOVA A,FERRER M D,et al.Effects of L-citrulline oral supplementation on polymorphonuclear neutrophils oxidative burst and nitric oxide production after exercise.[J].Free Radical Research,2009,43(9):828-835.
[12] HUDSON M B,HOSICK P A,MCCAULLEY G O,et al.The effect of resistance exercise on humoral markers of oxidative stress[J].Medicine&Science in Sports&Exercise,2008,40(3):542-548.
[13] KOLISEK M,MONTEZANO A C,SPONDER G,et al.PARK7/DJ-1 dysregulation by oxidative stress leads to magnesium deficiency:Implications in degenerative and chronic diseases[J].Clinical Science,2015,129(12)1143-1150.
[14] WU G,KNABE D A,KIM S W.Arginine nutrition in neonatal pigs[J].Journal of Nutrition,2004,134(10):2783-2790.
[15] 姚康,褚武英,邓敦,等.不同精氨酸添加水平对哺乳仔猪生长性能的影响[J].天然产物研究与开发,2008,1:121-124. YAO K,CHU W Y,DEN D,et al.Effects of different dietary arginine supplementation on sucking piglets'growth performance[J].Natural Product Research and Development,2008,1:121-124.(in Chinese)
[16] 李晓斌,赵国栋,邱昊日,等.L-瓜氨酸对小鼠体重及肠道菌群多样性的影响[J].动物营养学报,2019,31(10):1-7. LI X B,ZHAO G D,QIU H R,et al.Effects of L-citrulline on body weight and intestinal flora diversity of mice[J].Chinese Journal of Animal Nutrition,2019,31(10):1-7.(in Chinese)
[17] NORIS M,REMUZZI G.Physiology and pathophysiology of nitric oxide in chronic renal disease[J].Proceedings of the Association of American Physicians,1999,111(6):602-610.
[18] SCHWEDHELM E,MAAS R,FREESE R,et al.Pharmacokinetic and pharmacodynamic properties of oral L-citrulline and L-arginine:Impact on nitric oxide metabolism[J].British Journal of Clinical Pharmacology,2008,65(1):51-59.
[19] ELHATTAB A W,HSU J W,EMRICK L T,et al.Restoration of impaired nitric oxide production in MELAS syndrome with citrulline and arginine supplementation[J].Molecular Genetics&Metabolism,2012,105(4):607-614.
[20] 张仕琦,李晓斌,张文杰,等.补喂L-精氨酸或N-氨甲酰谷氨酸对伊犁马运动性能和血浆生化指标的影响[J].动物营养学报,2019,31(7):1-9. ZHANG S Q,LI X B,ZHANG W J,et al.Effects of supplemented with L-arginine or N-carbamylglutamate on athletic performance and plasma biochemical indexes of Yili horses[J].Chinese Journal of Animal Nutrition,2019.31(7):1-9.(in Chinese)
[21] 赵国栋,李晓斌,张涛,等.L-瓜氨酸对小鼠力竭运动性能及强运动应激后血清抗氧化能力和精液品质的影响[J].中国畜牧杂志,2019,网络首发. ZHAO G D,LI X B,ZHANG T,et al.Effects of L-citrulline on exhaustion performance and after strenuous exercise stress serum on antioxidant capacity and semen quality of mice[J].Chinese Journal of Animal Science,2019,Epub ahead of print.(in Chinese)
[22] ULRICHl F,LI H.Therapeutic effect of enhancing endothelial nitric oxide synthase (eNOS) expression and preventing eNOS uncoupling[J].British Journal of Pharmacology,2011,164(2):213-223.
[23] THIAGO SÁ,CLÁUDIA M M,BHAMBHANI Y N,et al.L-Arginine as a potential ergogenic aidin healthy subjects[J].Sports Medicine,2011,41(3):233-248.
[24] FORSTERMANN U.Endothelial nitric oxide synthase in vascular disease:From marvel to menace[J].Circulation,2006,113(13):1708-1714.