肉鸡养殖场中耐药相关基因散播情况的调查研究

doi:10.16431/j.cnki.1671-7236.2020.03.033

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (3): 931-939.doi: 10.16431/j.cnki.1671-7236.2020.03.033

肉鸡养殖场中耐药相关基因散播情况的调查研究

陈霞¹, 车洁¹, 骆鹏杰², 张云飞¹, 赵晓菲¹, 袁敏¹, 白雪梅¹, 李文革¹, 李娟¹

1. 中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室, 感染性疾病诊治协同创新中心, 北京 102206;
2. 国家食品安全风险评估中心, 北京 100022

收稿日期:2019-09-24 出版日期:2020-03-20 发布日期:2020-03-17
通讯作者: 李娟 E-mail:lijuan@icdc.cn
作者简介:陈霞(1983-),女,天津人,博士,研究方向:细菌耐药性,E-mail:chenxia@icdc.cn
基金资助:
"十三五"国家科技重大专项"病原菌耐药性检测、监测关键技术及传播规律研究"（2018XZ10733-402）；国家自然科学基金"肉鸡养殖场中ISCR1元件及其相关耐药基因的扩散风险研究"（31502124）

Investigation on the Spread of Resistance-related Genes in Broiler Farms

CHEN Xia¹, CHE Jie¹, LUO Pengjie², ZHANG Yunfei¹, ZHAO Xiaofei¹, YUAN Min¹, BAI Xuemei¹, LI Wenge¹, LI Juan¹

1. State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2. China National Center for Food Safety Risk Assessment, Beijing 100022, China

Received:2019-09-24 Online:2020-03-20 Published:2020-03-17

摘要/Abstract

摘要： 为研究耐药相关基因在肉鸡养殖场中的分布流行情况，本研究以河北省肉鸡养殖场待出栏肉鸡为研究对象，采用随机抽样方式采集肉鸡泄殖腔拭子样品264份，粪便污染地面样品9份，进行bla_NDM、bla_OXA、bla_CTX-M、armA、fexA、cfr、mcr-1、qnrS 8种耐药基因和int1、ISCR1 2种与耐药基因散布密切相关基因的实时荧光定量PCR检测。结果显示，10种基因在273份样品中均有不同程度检出，检出率在4.03%~97.07%之间，检出率最低的为armA基因，最高的为int1基因；样品中有基因共存情况，并以3种耐药基因共存的情况最多，有4份样品存在1种耐药基因，6份样品未检出任何耐药基因，没有样品共存8种耐药基因；通过数据分析，共得到41种不同的耐药基因谱型，其中常见的耐药基因谱型为bla_CTX-M-fexA-mcr-1和bla_OXA-bla_CTX-M-fexA-cfr-mcr-1-qnrS；分析发现样品携带两种及以上耐药基因时，与耐药基因散布相关的int1和ISCR1元件检出率越高，耐药基因在样品中检出的种类越多；耐药基因bla_OXA、bla_CTX-M、fexA、cfr、mcr-1、qnrS与int1基因的共存有统计学意义（P<0.05）；耐药基因bla_NDM、bla_OXA、bla_CTX-M、fexA、cfr、qnrS与ISCR1元件的共存有统计学意义（P<0.05）。本研究为畜牧兽医养殖行业耐药性监测工作提供了基于样品的新型实时荧光定量PCR检测方法，为有效预防耐药菌、耐药基因产生、控制耐药性的快速传播提供了可靠的数据支持。

关键词: 肉鸡; 耐药基因; 基因散播; 相关性

Abstract: To investigate resistance genes and their spread related genes in broilers,totally 273 broiler fecal samples were collected by random sampling from two broiler farms in Hebei province.A total of eight kinds of resistance genes and two kinds of spread related genes including bla_NDM,bla_OXA,bla_CTX-M,armA,fexA,cfr,mcr-1,qnrS,and int1,ISCR1 which closely related to the spread of resistance gene,were detected by Real-time PCR,respectively.According to the results,all of the genes were existed in the colaca swab samples with positive rate 4.03%-97.07%.The aminoglycoside-resistance gene,armA,showed the lowest positive rate,and resistance gene spread related gene,int1,showed the highest positive rate.More than two (including two) kinds of resistance genes were detected in 273 of samples.Three kinds of resistance genes co-existing was the most common.Four samples harbored only one resistance gene,and six harbored none.No sample harbored eight kinds of resistance genes.There were 41 resistance gene type profiles through data analysis.The common resistance gene type profiles included bla_CTX-M-fexA-mcr-1 and bla_OXA-bla_CTX-M-fexA-cfr-mcr-1-qnrS.Based on statistics analysis,the more resistance genes detected,the higher int1/ISCR1 gene positive rate exhibited in samples which carried more than two (including two) kinds of resistance genes.The coexistence of resistance genes bla_OXA,bla_CTX-M,fexA,cfr,mcr-1,qnrS with int1 gene showed statistical significance (P<0.05),respectively.And,the coexistence of resistance genes bla_NDM,bla_OXA,bla_CTX-M,fexA,cfr,mcr-1,qnrS with ISCR1 gene showed statistical significance (P<0.05),respectively.This study provided new Real-time PCR methods for drug resistance surveillance of animal husbandry and veterinary realms,and gave data support to drug resistance prevention and control in future.

Key words: broiler; resistance gene; gene spread; relationship

中图分类号:

S831

陈霞, 车洁, 骆鹏杰, 张云飞, 赵晓菲, 袁敏, 白雪梅, 李文革, 李娟. 肉鸡养殖场中耐药相关基因散播情况的调查研究[J]. 中国畜牧兽医, 2020, 47(3): 931-939.

CHEN Xia, CHE Jie, LUO Pengjie, ZHANG Yunfei, ZHAO Xiaofei, YUAN Min, BAI Xuemei, LI Wenge, LI Juan. Investigation on the Spread of Resistance-related Genes in Broiler Farms[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 931-939.

参考文献

[1] NHUNG N T,CUONG N V,THWAITES G,et al.Antimicrobial usage and antimicrobial resistance in animal production in southeast Asia:A review[J].Antibiotics (Basel),2016,5(4):E37.
[2] 朱冬梅,彭珍,刘书亮,等.肉鸡屠宰加工过程中沙门氏菌的污染情况及耐药性分析[J].食品科学,2014,35(17):214-219. ZHU D M,PENG Z,LIU S L,et al.Contamination and antimicrobial resistance patterns of Salmonella spp.from broiler slaughter and processing chain[J].Food Science,2014,35(17):214-219.(in Chinese)
[3] MAHIZAN N A,YANG S K,MOO C L,et al.Terpene derivatives as a potential agent against antimicrobial resistance (AMR) pathogens[J].Molecules,2019,24(14):2631.
[4] PARTRIDGE S R,KWONG S M,FIRTH N,et al.Mobile genetic elements associated with antimicrobial resistance[J].Clinical Microbiology Reviews,2018,31(4):e000088-17.
[5] 龚林,袁敏,陈霞,等.氨基糖苷类药物耐药基因armA实时荧光定量聚合酶链反应检测方法的建立[J].疾病监测,2014,29(11):901-904. GONG L,YUAN M,CHEN X,et al.Establishment of Real-time PCR assay to detect resistance gene armA[J].Disease Surveillance,2014,29(11):901-904.(in Chinese)
[6] CHEN X,ZHAO X F,CHE J,et al.Detection and dissemination of the colistin resistance gene,mcr-1,from isolates and feacal samples in China[J].Journal of Medical Microbiology,2017,66(2):119-125.
[7] 车洁,赵晓菲,卢金星,等.MGB探针标记-实时荧光PCR方法检测细菌int1基因和ISCR1元件方法的建立[J].疾病监测,2017,32(10):878-882. CHE J,ZHAO X F,LU J X,et al.Establishment of a duplex MGB Real-time PCR assay for int1 and ISCR1 element detections[J].Disease Surveillance,2017,32(10):878-882.(in Chinese)
[8] 陈霞,袁敏,李桂喜,等.基因捕获元件int1和ISCR1在临床菌株中的分布及细菌耐药性的关系研究[J].中国人兽共患病学报,2013,29(7):646-652. CHEN X,YUAN M,LI G X,et al.Dissemination of int1 gene and ISCR1 and their relations with multi-drug resistance in clinical isolates[J].Chinese Journal of Zoonoses,2013,29(7):646-652.(in Chinese)
[9] SHIN B,PARK W.Zoonotic diseases and phytochemical medicines for microbial infections in veterinary science:Current state and future perspective[J].Frontiers in Veterinary Science,2018,5:166.
[10] PETCHIAPPAN A,CHATTERJI D.Antibiotic resistance:Current perspectives[J].ACS Omega,2017,2(10):7400-7409.
[11] LIU Y Y,WANG Y,WALSH T R,et al.Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China:A microbiological and molecular biological study[J].Lancet Infect Disease,2016,16(2):161-168.
[12] CHEN X,LI G X,ZHANG H,et al.Characterization of class 1 integron gene cassettes among clinical bacteria isolated from one large hospital in northern China[J].Biomedical and Environmental Sciences,2013,26(12):1003-1007.
[13] DOMINGUEZ J E,REDONDO L M,FIGUEROA ESPINOSA R A,et al.Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry[J].Frontiers in Microbiology,2018,25(9):1679.
[14] MOUICHE M M M,MOFFO F,AKOACHERE J T K,et al.Antimicrobial resistance from a one health perspective in Cameroon:A systematic review and meta-analysis[J].BMC Public Health,2019,19(1):1135.
[15] 王迪轩,高东阳,张天姿,等.华中地区鸡源沙门菌分离鉴定及耐药性分析[J].中国畜牧兽医,2019,46(3):931-939. WANG D X,GAO D Y,ZHANG T Z,et al.Isolation,identification and drug resistance analysis of avian Salmonella in central China[J].China Animal Husbandry & Veterinary Medicine,2019,46(3):931-939.(in Chinese)
[16] 徐睿,李颜桃.鸡源致病性奇异变形杆菌的分离鉴定与遗传进化分析[J].中国畜牧兽医,2018,45(9):2550-2558. XU R,LI Y T.Isolation,identification and genetic evolution analysis of chicken pathogenic Proteus mirabilis[J].China Animal Husbandry & Veterinary Medicine,2018,45(9):2550-2558.(in Chinese)
[17] 马馨,刘一飞,王志宇,等.鸡源大肠杆菌分离鉴定及ESBLs与AmpC酶基因型检测及耐药性分析[J].中国畜牧兽医,2019,46(9):2715-2725. MA X,LIU Y F,WANG Z Y,et al.Isolation,identification,detection of ESBLs and AmpC enzyme genotypes and drug resistance analysis of Escherichia coli from chicken[J].China Animal Husbandry & Veterinary Medicine,2019,46(9):2715-2725.(in Chinese)
[18] SHEN Z Q,WANG Y,SHEN Y B,et al.Early emergence of mcr-1 in Escherichia coli from food-producing animals[J].Lancet Infectious Diseases,2016,16(3):293.
[19] WANG Y,ZHANG R,LI J,et al.Comprehensive resistome analysis reveals the prevalence of NDM and MCR-1 in Chinese poultry production[J].Nature Microbiology,2017,2:16260.
[20] COLELLO R,KRVGER A,CONZA J D,et al.Antimicrobial resistance in class 1 integron-positive Shiga toxin-producing Escherichia coli isolated from cattle,pigs,food and farm environment[J].Microorganisms,2018,6(4):99.
[21] TOLEMAN M A,BENNETT P M,WALSH T R.ISCR elements:Novel gene-capturing systems of the 21st century[J].Microbiology and Molecular Biology Reviews,2006,70(2):296-316.
[22] 陈霞,车洁,赵晓菲,等.483株肉鸡源大肠埃希菌和肺炎克雷伯菌中ISCR1及int1基因的流行及耐药情况[J].中华预防医学杂志,2017,51(10):886-889. CHEN X,CHE J,ZHAO X F,et al.Dissemination of insertion sequence common regions 1 and int1 gene and drug resistance of 483 Escherichia coli and Klebsiella pneumonia broiler isolates[J].Chinese Journal of Preventive Medicine,2017,51(10):886-889.(in Chinese)
[23] LI J,LAN R T,XIONG Y W,et al.Sequential isolation in a patient of Raoultella planticola and Escherichia coli bearing a novel ISCR1 element carrying bla_NDM-1[J].PLoS One,2014,9(3):e89893.

肉鸡养殖场中耐药相关基因散播情况的调查研究

Investigation on the Spread of Resistance-related Genes in Broiler Farms

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