绵羊GLP2R基因多态性及其与肉质性状的相关分析

doi:10.16431/j.cnki.1671-7236.2019.11.021

摘要/Abstract

摘要： 为探索胰高血糖素样肽-2受体（glucagon-like peptide-2 receptor，GLP2R）基因多态性与绵羊肉质性状的相关性，试验选用68只南非肉用美利奴羊×东北细毛羊和102只杜泊羊×小尾寒羊的杂交后代为研究对象，采用Sanger测序方法寻找GLP2R基因外显子11序列的突变位点，根据测序结果分析该突变位点的基因型频率和基因频率及卡方适合性检验、纯合度（Ho）、杂合度（He）和多态信息含量（PIC）。结果显示，绵羊GLR2R基因外显子11处存在C/T突变，存在3种基因型：CC、TC和TT，2种等位基因：C和T，其中在南非肉用美利奴羊×东北细毛羊群体中，TC基因型为优势基因型，在杜泊羊×小尾寒羊群体中，CC基因型为优势基因型；2个群体中C均为优势等位基因。卡方适合性检验结果显示，2个群体的C/T位点均处于Hardy-Weinberg平衡状态（P>0.05）；C/T突变位点在群体内变异较小且处于中度多态（0.25 < PIC < 0.5），有一定遗传学意义。不同基因型肉质性状差异结果显示，南非肉用美利奴羊×东北细毛羊TC基因型剪切力显著高于CC和TT基因型，TT基因型失水率显著高于CC和TC基因型（P<0.05）；杜泊羊×小尾寒羊CC基因型剪切力显著高于TC和TT基因型，TT基因型失水率显著高于CC和TC基因型（P<0.05）。本研究在GLP2R基因外显子处发现C/T突变，可能是影响肉质性状的潜在位点，是否能够作为遗传标记还需要进一步探索。

关键词: 南非肉用美利奴羊×东北细毛羊; 杜泊羊×小尾寒羊; GLP2R基因; 多态性; 肉质性状

Abstract: To explore the correlation between glucagon-like peptide-2 receptor (GLP2R) gene polymorphism and meat quality traits in sheep,the hybrid offspring of 68 South African meat Merino sheep×Northeast Fine wool sheep and 102 Dorper sheep×Small-tail Han sheep as the research object in this study,the Sanger sequencing method was used to find the mutation sites of the sequences of GLP2R gene exon 11,according to the sequencing results,the genotype frequency and gene frequency of the mutation site and the chi-square suitability test,homozygosity (Ho),heterozygosity (He) and polymorphism information content (PIC) were analyzed.The results showed that there were C/T mutations of GLR2R gene exon 11 in sheep,there were three genotypes (CC,TC and TT) and two alleles (C and T).TC genotype was the dominant genotype in South Africa meat Merino sheep×Northeast Fine wool sheep,CC genotype was the dominant genotype in Dorper sheep×Small-tail Han sheep,and C allele was dominant in both two populations.The results of chi-square suitability test showed that C/T site in two populations were in Hardy-Weinberg equilibrium (P>0.05).The intra-population variation was small and moderate polymorphism (0.25 < PIC < 0.5),which had certain genetic significance.The results of different genotypes of meat quality traits showed that the shearing force of TC genotype in South African meat Merino sheep×Northeast Fine wool sheep was significantly higher than that of CC and TT genotypes,and the water loss rate of TT genotype was significantly higher than that of CC and TC genotypes (P<0.05);The shearing force of CC genotype in Dorper sheep×Small-tail Han sheep was significantly higher than that of TC and TT genotypes,and the water loss rate of TT genotype was significantly higher than that of CC and TC genotypes (P<0.05).In this study,a C/T mutation was found in GLP2R gene exon,which might be a potential site affecting meat quality traits,whether it could be used as a genetic marker needed further exploration.

Key words: South African meat Merino sheep×Northeast Fine wool sheep; Dorper sheep×Small-tail Han sheep; GLP2R gene; polymorphism; meat quality traits

中图分类号:

S813.3

梁祎凡, 曹阳, 金花子, 魏天, 肖成, 马惠海, 金海国. 绵羊GLP2R基因多态性及其与肉质性状的相关分析[J]. 中国畜牧兽医, 2019, 46(11): 3316-3322.

LIANG Yifan, CAO Yang, JIN Huazi, WEI Tian, XIAO Cheng, MA Huihai, JIN Haiguo. Correlation Analysis Between GLP2R Gene Polymorphism and Meat Quality Traits in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3316-3322.

参考文献

[1] LEE S J,LEE J,LI K K,et al.Disruption of the murine GlP2R impairs Paneth cell function and increases susceptibility to small bowel enteritis[J].Endocrinology,2012,153(3):1141-1151.
[2] IRWIN D M,MOJSOV S.Diversification of the functions of proglucagon and glucagon receptor genes in fish[J].General and Comparative Endocrinology,2018,261:148-165.
[3] 刘理想,孙大明,毛胜勇,等.胰高血糖素样肽-2对羔羊胃肠道重量、瘤胃发酵及小肠上皮发育相关基因表达的影响[J].畜牧兽医学报,2018,49(3):549-558. LIU L X,SUN D M,MAO S Y,et al.Effects of glucagon-like peptide-2 on gastrointestinal weight,rumen fermentation and expression of small intestinal epithelial development-related genes in lambs[J].Acta Veterinaria et Zootechnica Sinica,2018,49(3):549-558.(in Chinese)
[4] XIE Y C,YAO Z H,YAO X L,et al.Glucagon-like peptide-2 receptor is involved in spatial cognitive dysfunction in rats after chronic cerebral hypoperfusion[J].Journal of Alzheimer's Disease,2018,66(4):1559-1576.
[5] WISMANN P,BARKHOLT P,SECHER T,et al.The endogenous preproglucagon system is not essential for gut growth homeostasis in mice[J].Molecular Metabolism,2017,6(7):681-692.
[6] LUO W,CHENG D,CHEN S,et al.Genome-wide association analysis of meat quality traits in a porcine Large White×Minzhu intercross population[J].International Journal of Biological Sciences,2012,8(4):580-595.
[7] 李津,朱雨霏,易志刚,等.胰升糖素样肽1受体激动剂调节糖尿病合并非酒精性肝病大鼠胰岛素抵抗及肝脏氧化应激损伤的实验研究[J].中华内分泌代谢杂志,2017,33(3):228-232. LI J,ZHU Y F,YI Z G,et al.Experimental study on the glucagon-like peptide 1 receptor agonist modulation of insulin resistance and hepatic oxidative stress in rats with diabetes mellitus combined with nonalcoholic fatty liver disease[J].Chinese Journal of Endocrine and Metabolism,2017,33(3):228-232.(in Chinese)
[8] BALDASSANO S,AMATO A,CAPPELLO F,et al.Glucagon-like peptide-2 and mouse intestinal adaptation to a high-fat diet[J].The Journal of Endocrinology,2013,217(1):11-20.
[9] YUSTA B,MATTHEWS D,FLOCK G B,et al.Glucagon-like peptide-2 promotes gallbladder refilling via a TGR5-independent,GLP-2R-dependent pathway[J].Molecular Metabolism,2017,6(6):503-511.
[10] LI F,LU L,PENG Y,et al.Increased GLP2R expression in gastric chief cells of patients with severe obesity regardless of diabetes status[J].International Journal of Obesity:Journal of the International Association for the Study of Obesity,2017,41(8):1303-1305.
[11] 朱红霞,邓晓龙,高静,等.利拉鲁肽对糖尿病合并脂肪肝大鼠的脂代谢相关基因表达的影响[J].中国临床研究,2017,30(1):7-10. ZHU H X,DENG X L,GAO J,et al.Effects of liraglutide on expressions of lipid metabolism-related genes in rats with T2DM and fatty liver[J]. Chinese Journal of Clinical Research,2017,30(1):7-10.(in Chinese)
[12] PARK C R,MOON M J,PARK S,et al.A novel glucagon-related peptide (GCRP) and its receptor GCRPR account for coevolution of their family members in vertebrates[J].Accelerating the Publication of Peer-Reviewed Science,2013,8(6):e65420.
[13] 齐珂珂,徐子伟,门小明.胰高血糖素样肽-2受体在新生仔猪部分内脏器官中的分布[J].中国畜牧杂志,2013,49(7):56-59. QI K K,XU Z W,MEN X M.Distribution of glucagon-like peptide-2 receptor in some internal organs of newborn piglets[J]. Chinese Journal of Animal Science,2013,49(7):56-59.(in Chinese)
[14] CAO M,CHE L,WANG J,et al.Effects of maternal over-and undernutrition on intestinal morphology,enzyme activity,and gene expression of nutrient transporters in newborn and weaned pigs[J].Nutrition,2014,30(11-12):1442-1447.
[15] GUAN X,SHI X,LI X,et al.GLP-2 receptor in POMC neurons suppresses feeding behavior and gastric motility[J].American Journal of Physiology Endocrinology and Metabolism,2012,303(7):853-864.
[16] GUAN X.The CNS glucagon-like peptide-2 receptor in the control of energy balance and glucose homeostasis[J].American Journal of Physiology,Regulatory,Integrative and Comparative Physiology,2014,307(6):R585-R596.
[17] MO C,ZHONG Y,WANG Y,et al.Characterization of glucagon-like peptide 2 receptor (GLP2R) gene in chickens:Functional analysis,tissue distribution,and developmental expression profile of GLP2R in embryonic intestine[J]. Domestic Animal Endocrinology,2014,48:1-6.
[18] TAHER J,BAKER C,ALVARES D,et al.GLP-2 dysregulates hepatic lipoprotein metabolism,inducing fatty liver and VLDL overproduction in male hamsters and mice[J].Endocrinology,2018,159(9):3340-3350.
[19] 王静,马玉宝.胰高血糖素样肽1/胰高血糖素受体双重激动剂治疗肥胖症研究进展[J].中华保健医学杂志,2018,20(1):81-83. WANG J,MA Y B.Progress in the treatment of obesity with glucagon-like peptide 1/glucagon receptor dual agonist[J].Chinese Journal of Health Care and Medicine,2018,20(1):81-83.(in Chinese)
[20] LIU X,WANG L G,LUO W Z,et al.Genome-wide SNP scan in a porcine Large White×Minzhu intercross population reveals a locus influencing muscle mass on chromosome 2[J]. Animal Science Journal,2014,85(12):969-975.
[21] 杨广礼,付冬丽,郎侠,等.中国地方绵羊群体TYRP1基因遗传变异研究[J].中国畜牧兽医,2018,45(10):2772-2786. YANG G L,FU D L,LANG X,et al.Study on genetic variation of TYRP1 gene in Chinese indigenous sheep populations[J].China Animal Husbandry & Veterinary Medicine,2018,45(10):2772-2786.(in Chinese)
[22] FENG Y,DEMEHRI F R,XIAO W,et al.Interdependency of EGF and GLP-2 signaling in attenuating mucosal atrophy in a mouse model of parenteral nutrition[J].Cellular and Molecular Gastroenterology and Hepatology,2017,3(3):447-468.
[23] QI K K,SUN Y Q,WAN J,et al.Effect of porcine glucagon-like peptides-2 on tight junction in GLP-2R+IPEC-J2 cell through the PI3 k/Akt/mTOR/p70S6K signalling pathway[J].Journal of Animal Physiology and Animal Nutrition,2017,101(6):1242-1248.
[24] CHAI Y L,MA H M,JIANG J.Molecular characterization,tissue expression profile and SNP analysis of porcine GLP2R[J].Genetics and Molecular Research,2015,14(4):12931-12941.