口蹄疫疫苗非抗原蛋白对146S抗原免疫效果的影响

doi:10.16431/j.cnki.1671-7236.2019.03.032

摘要/Abstract

摘要：

为探究非抗原蛋白对口蹄疫病毒A型（FMDV-A）146S抗原免疫效果的影响，本研究以小鼠和猪为试验动物，分别制备5组小鼠注射用样品：A组（3 μg 146S抗原）、B组（3 μg 146S抗原+25 μg非抗原蛋白）、C组（3 μg 146S抗原+50 μg非抗原蛋白）、D组（3 μg 146S抗原+100 μg非抗原蛋白）及E组（空白对照，PBS）；同时制备4组猪注射用样品：A组（18 μg 146S抗原）、B组（18 μg 146S抗原+400 μg非抗原蛋白）、C（18 μg 146S抗原+4 000 μg非抗原蛋白）及D组（空白对照，PBS）。免疫接种试验动物后，通过淋巴细胞增殖试验及实时荧光定量PCR评价比较不同疫苗样品组小鼠产生的细胞免疫应答水平，同时通过液相阻断ELISA方法检测、评价各试验组动物（小鼠和猪）产生的体液免疫应答水平。小鼠试验结果显示，在免疫后3个检测时间点内，4个试验组中C组的平均抗体水平最高，平均抗体效价分别为4.13、5.83和5.50，而D组的抗体水平最低，平均抗体效价分别为3.46、5.16和4.46；C组细胞增殖能力及Th-1型细胞因子IL-6、IFN-β和TNF-α mRNA表达量均高于其他组。猪试验结果显示，A组和B组间平均抗体水平差异不显著（P>0.05），但A组和B组的平均抗体效价均极显著高于C组（P<0.01）。综合上述结果表明，一定范围内少量非抗原蛋白对FMDV-A 146S抗原的免疫效果没有影响，而高浓度的非抗原蛋白则抑制FMDV-A 146S抗原的免疫效果。

关键词: 非抗原蛋白; 口蹄疫; 146S抗原; 免疫效果

Abstract:

In order to explore the effect of non-antigen protein on the immune effect of foot-and-mouth disease virus type A (FMDV-A) 146S antigen,mice and pigs were used as experimental animals.5 groups of mice for injection were prepared:Group A (3 μg 146S antigen),group B (3 μg 146S antigen＋25 μg non-antigenic protein),group C (3 μg 146S antigen＋50 μg non-antigenic protein),group D (3 μg 146S antigen＋100 μg non-antigenic protein) and group E (blank control,PBS).At the same time,four groups of pig injection samples were prepared:Group A (18 μg 146S antigen),group B (18 μg 146S antigen＋400 μg non-antigenic protein),group C (18 μg 146S antigen＋4 000 μg non-antigenic protein) and group D (blank control,PBS).The cell immune response levels of mice in different vaccine sample groups were compared by lymphocyte proliferation assay and Real-time PCR.The level of humoral immune response produced by the animals (mice and pigs) of each test group was detected and evaluated by a liquid phase blocking ELISA method.The results of mice test showed that the average antibody level of group C was the highest in four test groups at the three test time points after immunization,and the average antibody titers were 4.13,5.83 and 5.50,respectively,while the antibody of group D was the lowest,the average antibody titer average antibody titers were 3.46,5.16 and 4.46 in the antibody detection,respectively;The cell proliferation ability and Th-1 type cytokine IL-6,IFN-β and TNF-α mRNA expression in group C were higher than other groups.The results of pigs test showed that the average antibody level between groups A and B was not significantly different (P>0.05),but the average antibody titers of groups A and B were extremely significantly higher than that of group C (P<0.01).The above results indicated that a small amount of non-antigenic protein had no effect on the immune effect of FMDV-A 146S antigen within a certain range,while a high concentration of non-antigenic protein inhibited the immune effect of FMDV-A 146S antigen.

Key words: non-antigenic proteins; foot-and-mouth disease; 146S antigen; immune effect

中图分类号:

S852.4

李明, 张雅娜, 郭晓宇, 高新桃, 李金祥, 朱鸿飞. 口蹄疫疫苗非抗原蛋白对146S抗原免疫效果的影响[J]. 《中国畜牧兽医》, 2019, 46(3): 905-912.

LI Ming, ZHANG Yana, GUO Xiaoyu, GAO Xintao, LI Jinxiang, ZHU Hongfei. Effect of Non-antigen Protein of Foot-and-Mouth Disease Vaccine on Immune Effect of 146S Antigen[J]. , 2019, 46(3): 905-912.

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