鸡新城疫－传染性支气管炎－传染性法氏囊病三联灭活疫苗对不同日龄雏鸡的免疫效力研究

doi:10.16431/j.cnki.1671-7236.2019.03.031

《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (3): 898-904.doi: 10.16431/j.cnki.1671-7236.2019.03.031

鸡新城疫－传染性支气管炎－传染性法氏囊病三联灭活疫苗对不同日龄雏鸡的免疫效力研究

沈佳, 史爱华, 章振华, 李林, 景小冬, 张建伟, 郑小兰, 姜北宇

北京市农林科学院畜牧兽医研究所, 畜禽疫病防控技术北京市重点实验室, 北京 100097

收稿日期:2018-10-08 出版日期:2019-03-20 发布日期:2019-03-20
通讯作者: 章振华 E-mail:zhangzhenhua304@163.com
作者简介:沈佳(1985-),女,北京人,硕士,助理研究员,研究方向:禽用疫苗的工艺技术,E-mail:gracekent@163.com;史爱华(1978-),女,满族,北京人,硕士,助理研究员,研究方向:畜禽疫病防控,E-mail:shiaihua78@163.com
基金资助:
北京市农林科学院创新能力建设项目（KJCX20161503、KJCX20180419、JNKYT201602）

Study on the Immune Efficacy of A Trivalent Inactivated Vaccine Against Newcastle Disease, Infectious Bronchitis and Infectious Bursal Disease in Different Days Old Chicks

SHEN Jia, SHI Aihua, ZHANG Zhenhua, LI Lin, JING Xiaodong, ZHANG Jianwei, ZHENG Xiaolan, JIANG Beiyu

Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Municipal Academy of Agriculture and Forestry, Beijing 100097, China

Received:2018-10-08 Online:2019-03-20 Published:2019-03-20

摘要/Abstract

摘要：

为评估鸡新城疫（ND）-传染性支气管炎（IB）-传染性法氏囊病（IBD）三联灭活疫苗对不同日龄和不同水平母源抗体雏鸡的免疫效力和持续期，本试验用该疫苗免疫7、14、21日龄SPF雏鸡和有母源抗体的普通雏鸡，免疫后采血测定ND血凝抑制抗体（HI Ab）、IB血凝抑制抗体（HI Ab）及IBD中和抗体（NA），并用传染性法氏囊病病毒（IBDV）强毒攻击。结果显示，7日龄SPF雏鸡免疫后21 d ND HI抗体、IB HI抗体及IBD中和抗体效价分别为7.9log2、6.9log2和14.1log2，SPF鸡日龄越大，抗体水平越高；28日龄SPF鸡免疫后3个月，0.3 mL免疫剂量组试验鸡ND HI、IB HI及IBD中和抗体效价分别达6.5log2、6.1log2和13.6log2，IBDV攻毒保护率均为100%（10/10）；不同日龄普通雏鸡免疫效果与SPF鸡试验一致，抗体水平随鸡日龄增大而升高，IBD攻毒保护率也都达到100%（10/10）。试验结果证实，鸡新城疫-传染性支气管炎-传染性法氏囊病三联灭活疫苗可使7、14及21日龄SPF雏鸡和普通雏鸡产生良好的免疫力，对雏鸡的免疫期至少为3个月。

关键词: 新城疫(ND); 传染性支气管炎(IB); 传染性法氏囊病(IBD); 三联灭活疫苗; 免疫效力; 免疫持续期

Abstract:

To evaluate immune efficacy and immune duration of a trivalent inactivated vaccine against Newcastle disease (ND),infectious bronchitis (IB) and infectious bursal disease (IBD),the vaccine was administered to SPF and common healthy chicks with maternal antibodies at age of 7,14 and 21 days old.The blood samples of the chicks were collected at 21 d after vaccination,then ND haemagglutination inhibition antibody (HI Ab),IB HI Ab and IBD neutralizing antibody (NA) of the blood samples were examined,and virulent IBDV challenge were carried out.The results showed that the ND HI Ab,IB HI Ab and IBD NA titers of SPF chicks at 7 days old were 7.9log2,6.9log2 and 14.1log2,respectively,and the older the SPF chicks,the higher the antibody level.Three months after immunization,the ND HI Ab,IB HI Ab and IBD NA titers of chicks immused with 3 mL vaccine reached 6.5log2,6.1log2 and 13.6log2 respectively,and the protection rate against IBDV challenge was 100% (10/10).The immune effect of common chicks was almost similar with SPF chicks,the antibody levels increased with the age of the chicks,and the protection rate against IBDV was 100% (10/10).The results confirmed that the different days old chicks inoculated with the trivalent inactivated vaccine could produce good immunity to against virulent IBDV challenge,the immunity duration of the triple inactivated vaccine was 3 months at least.

Key words: Newcastle disease (ND); infectious bronchitis (IB); infectious bursal disease (IBD); trivalent inactivated vaccine; immune efficacy; immune duration

中图分类号:

S858.31

沈佳, 史爱华, 章振华, 李林, 景小冬, 张建伟, 郑小兰, 姜北宇. 鸡新城疫－传染性支气管炎－传染性法氏囊病三联灭活疫苗对不同日龄雏鸡的免疫效力研究[J]. 《中国畜牧兽医》, 2019, 46(3): 898-904.

SHEN Jia, SHI Aihua, ZHANG Zhenhua, LI Lin, JING Xiaodong, ZHANG Jianwei, ZHENG Xiaolan, JIANG Beiyu. Study on the Immune Efficacy of A Trivalent Inactivated Vaccine Against Newcastle Disease, Infectious Bronchitis and Infectious Bursal Disease in Different Days Old Chicks[J]. , 2019, 46(3): 898-904.

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鸡新城疫－传染性支气管炎－传染性法氏囊病三联灭活疫苗对不同日龄雏鸡的免疫效力研究

Study on the Immune Efficacy of A Trivalent Inactivated Vaccine Against Newcastle Disease, Infectious Bronchitis and Infectious Bursal Disease in Different Days Old Chicks

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