关键词: 狂犬病SRV₉病毒; 复合佐剂; 口服疫苗诱饵; 免疫效果

Abstract:

The study was aimed to select the best composite bait adjuvants and oral vaccine prescriptions, and test the immune mice serum IgG and IgA antibody titer in the gut. Using the beef powder, chicken powder, fish powder, milk powder and blood meal as the main raw material, and bacterial lipopolysaccharide, zinc hydroxide, LBP, mannan oligosaccharide as vaccine adjuvant,through orthogonal experiment method composited immunologic adjuvant and oral rabies vaccine bait SRV₉, after mixing virus oral immunization of test mice. The optimized composite adjuvant party was bacterial lipopolysaccharide 100 μg, zinc hydroxide 0.8 mg, LBP 50 mg, mannan oligosaccharides 10 mg, serum IgG antibody content of mice immunized with 3.24 mg/L, intestinal IgA antibody content of 1.56 ng/mL.In feeding trials of the vaccine in the bait, wolves, foxes, dogs and cats feed on the bait fish flavor highest rate, followed by dried blood and creamy bait; After bait mixed with the rabies virus SRV₉ oral immunization of mice 28 d the serum IgG was in average of 1.20 U/L. This experiment screened preparation of bait fish meat with high intake, was suitable for wild carnivorous animal oral vaccine preparation, and screened the compound oral vaccine adjuvant,it would increase the immune effect of rabies virus SRV₉ strain and provide the necessary technical support for further research and development of recombinant attenuated rabies vaccine.

Key words: rabies SRV₉ virus; composite adjuvant; oral vaccine bait; immune effect