山羊单增李斯特杆菌病的诊断与防治

doi:10.16431/j.cnki.1671-7236.2018.10.034

摘要/Abstract

摘要：

赣西某养殖场山羊出现转圈运动等神经症状、单侧眼睛失明和呼吸困难，导致近20只山羊死亡。为确定病原菌并进行有效的防治，对送检的3只病死羊进行病理剖检，并采集肝脏、脾脏和脑组织进行病原菌的纯化培养、LB二次增菌选择性分离培养、革兰氏染色、生化试验和16S rRNA测序分析；为确定病原菌致病力，进行小鼠和家兔的动物回归试验；选用头孢唑啉、头孢曲松钠、青霉素、万古霉素等30种抗菌药进行病原菌的体外抑菌试验。结果显示，从两只病羊的脑组织中分离得到两株革兰氏阳性细菌，生化试验、16S rRNA序列测定、动物回归试验确认分离菌为单增李斯特杆菌，二者高度同源（100.0%），与多株李氏杆菌同源性均高达99%以上，且对小鼠和家兔均有较强的致病力。该分离菌对头孢唑啉、哌拉西林、羧苄西林、四环素、多西环素、麦迪霉素、红霉素、万古霉素和氯霉素高度敏感，但对第2、3代头孢菌素类抗生素、苯唑西林、复方新诺明等抗菌药耐药。该养殖场应参考药敏试验结果采取综合防治措施，从而保障山羊及其肉制品的食品安全。

关键词: 山羊; 单增李斯特杆菌病; 单增李斯特杆菌; 药敏试验; 16S rRNA

Abstract:

The goats in a farm in Western Jiangxi showed neurological symptoms such as circling movements,unilateral blindness and dyspnea,nearly 20 goats died.In order to identify the pathogens and establish effective prevention and treatment measures,the pathological necropsy was performed on three dead sheep that were sent for inspection.The viscera tissues such as liver,spleen and brain tissues were collected for the purification and cultivation of pathogenic bacteria.And the LB medium was used to make a secondary enrichment isolation selectively.Gram staining,biochemical tests,and 16S rRNA gene sequencing analysis were carried out.The animal experiments in mice and rabbits were conducted in order to identify the pathogenicity of pathogenic bacteria.To identify the sensitivity of pathogenic bacteria to antibacterial drugs,cefazolin,ceftriaxone sodium,penicillin,vancomycin and other 30 antibacterial agents were used in bacteriostasis experiment in vitro of pathogenic bacteria.The results were as follows:Two strains of Gram-positive bacteria were isolated from the brain tissue of two sheep,both of which were identified as Listeria monocytogenes with strong virulence.The homology between two strains was 100.0%,and that of two strains and other referenced Listeria were higher than 99.0%.The two isolates were highly sensitive to drugs of cefazolin,piperacillin,carbenicillin,tetracyclines,doxycycline,aboren,erythromycin,vancomycin and chloroamphenicol.However,they were resistant to the 1st and 2nd generation cephalosporin antibiotics,oxacillin and PCST.This farm should adopt comprehensive prevention and control measures according to drug sensitivity test results to better control the disease in order to keep the safety of goats and meat products.

Key words: goat; listeriosis; Listeria monocytogenes; drug susceptibility test; 16S rRNA

中图分类号:

S852.61

唐慕德, 温宇婷, 吴青青, 阿依江·阿比提, 伍钢, KASSIM ALI BAKAR, 邓秋纯, 刘犇, 郑文亚. 山羊单增李斯特杆菌病的诊断与防治[J]. 《中国畜牧兽医》, 2018, 45(10): 2951-2959.

TANG Mude, WEN Yuting, WU Qingqing, AYIJIANG·ABITI, WU Gang, KASSIM ALI BAKAR, DENG Qiuchun, LIU Ben, ZHENG Wenya. Diagnosis and Treatment of Goat Listeriosis[J]. , 2018, 45(10): 2951-2959.

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