苦玄参提取物对仔猪血清生化指标及免疫指标的影响

doi:10.16431/j.cnki.1671-7236.2018.10.011

摘要/Abstract

摘要：

试验旨在探讨苦玄参提取物对仔猪血液生化指标、抗氧化功能和部分免疫指标的影响，为苦玄参提取物在仔猪生产中的合理利用提供理论依据。选取28~35日龄、体重相近的杜×长×大三元杂交断奶仔猪250头，随机分为5组（A、B、C、D、E组），每组5个重复，每个重复10头。其中A、B、C组依次为苦玄参提取物高、中、低剂量组，分别在基础日粮中添加3.5、1.75和0.875 g/kg苦玄参提取物；D组为药物对照组，在基础日粮中添加4.0 g/kg七补散；E组为空白对照组，饲喂基础日粮。预饲期7 d，正试期30 d。分别在用药14 d后和停药14 d后时，从每组中随机选取接近平均体重的10头仔猪于晨饲前进行前腔静脉采血，测定血清生化指标、抗氧化指标和免疫指标。结果显示：①各组仔猪血液生化指标差异均不显著（P>0.05），说明苦玄参提取物对仔猪体内糖代谢、脂代谢及消化系统无显著影响；②从抗氧化指标看，添加苦玄参提取物14 d后，B组一氧化氮（NO）含量极显著高于其他组（P<0.01），A组总抗氧化能力（T-AOC）极显著高于其他组（P<0.01），B、D组显著或极显著高于C、E组（P<0.05；P<0.01）；而停药14 d后，E组谷胱甘肽（GSH）含量显著高于B、C、D组，各组其余指标差异均不显著（P>0.05）；③从免疫指标看，添加苦玄参提取物14 d后，B组免疫球蛋白G（IgG）含量极显著高于其他组（P<0.01），A组白细胞介素6（IL-6）含量显著高于B、D、E组（P<0.05）；而停药14 d后，各组免疫指标差异不显著（P>0.05）；综上所述，日粮中添加0.875~3.5 g/kg苦玄参提取物对仔猪无不良影响，且可在一定程度上增强机体的抗氧化功能和机体免疫水平。

关键词: 仔猪; 苦玄参提取物; 血清生化指标; 抗氧化指标; 免疫指标

Abstract:

This trial was conducted to investigate the effect of dietary Picria fel-tarrae Lour extracts on serum biochemical indexes,anti-oxidant indexes and some immune indexes of piglets to provide theoretical basis and production guidance for rational utilization of Picria fel-terrae Lour extracts in the production of piglets.A total of 250 healthy cross-breed pigs (Duroc×Landrace×Yorkshire) at 28-35 days were selected and randomly divided into 5 groups (groups A,B,C,D and E) with 5 replicates per treatment and 10 piglets per replicate.The piglets in groups A,B and C were fed basal diet supplemented with high (3.5 g/kg),middle (1.75 g/kg)and low doses (0.875 g/kg) Picria fel-tarrae Lour extracts,respectively,that in group D fed basal diet with 4.0 g/kg Qibusan,while that in group E was fed basal diet.The pre-feeding period was 7 d and the trial period was 30 d.Blood samples were obtained from the anterior vena cava by randomly selecting 10 piglets from each group on the 14th day after administration and the 14th day after drug withdrawal to analyze the biochemical,antioxidant and immune indexes.The results showed as follows:①There was no significant difference in blood biochemical indexes among experimental groups,indicating Picria fel-terrae Lour extracts had no adverse effects on glucose metabolism,lipid metabolism and digestive system in piglets.②From the antioxidant indexes,after administration for 14 d,NO content in group B was extremely significantly higher than that of other groups (P < 0.01),and the contents of T-AOC in group A was extremely significantly higher than that of other groups (P < 0.01),and that in groups B and D were extremely significantly or significantly higher than groups C and E (P < 0.01;P < 0.05).At 14th day after drug withdrawal,the contents of GSH in group E was significantly higher than that of groups B,C and D (P < 0.05).There was no significant difference in other indexes among all groups (P > 0.05).③From the immune indexes,after administration for 14 d,the level of IgG in group B was extremely significantly higher than that in other groups (P < 0.01) and the content of IL-6 in group A was significantly higher than that in groups B,D and E (P < 0.05).At 14th day after drug withdrawal,there was no significant difference in immune indexes among all groups (P > 0.05).In conclusion,the dietary supplementation of 0.875 to 3.5 g/kg Picria fel-tarrae Lour extracts had a good effect on enhancing the antioxidant function and improving the immune level of piglets with no adverse effects on piglets health.

Key words: piglets; Picria fel-tarrae Lour extracts; serum biochemical indexes; antioxidant indexes; immune indexes

中图分类号:

S828

刘妍璨, 李晓玉, 陈忠伟, 段群棚, 张宁, 郭旋, 黄宏业, 胡庭俊, 何颖. 苦玄参提取物对仔猪血清生化指标及免疫指标的影响[J]. 《中国畜牧兽医》, 2018, 45(10): 2743-2751.

LIU Yancan, LI Xiaoyu, CHEN Zhongwei, DUAN Qunpeng, ZHANG Ning, GUO Xuan, HUANG Hongye, HU Tingjun, HE Ying. Effect of Dietary Picria fel-tarrae Lour. Extracts on Serum Biochemical Indexes and Immune Function of Piglets[J]. , 2018, 45(10): 2743-2751.

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