日粮精氨酸水平对蛋鸭卵泡膜血管形成相关基因及蛋白表达的影响

doi:10.16431/j.cnki.1671-7236.2018.02.012

《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (2): 384-391.doi: 10.16431/j.cnki.1671-7236.2018.02.012

日粮精氨酸水平对蛋鸭卵泡膜血管形成相关基因及蛋白表达的影响

夏伟光¹, 陈伟¹, 阮栋¹, 王爽¹, FOUAD A M^1,2, 罗茜¹, 熊云霞¹, 王胜林¹, 郑春田¹

1. 广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 农业部华南动物营养与饲料重点实验室, 广东省动物育种与营养公共实验室, 广东省畜禽育种与营养研究重点实验室, 广州 510640;
2. 开罗大学农学院动物生产系, 吉萨 12613

收稿日期:2017-07-21 出版日期:2018-02-20 发布日期:2018-02-10
通讯作者: 郑春田 E-mail:zhengcht@163.com
作者简介:夏伟光(1985-),男,广东清远人,博士,研究方向:水禽营养与饲料资源利用技术,E-mail:harry_xch@sina.com
基金资助:
国家水禽产业技术体系（CARS-43-13）；广东省科技计划项目（2011A020102009、2016A020210043）；广东省自然科学基金博士启动项目（2014A030310054）；广东省农业科学院院长基金项目（201521）；广东省畜禽育种与营养研究重点实验室运行经费（2014B030301054）

Effects of Dietary Arg Levels on Angiopoiesis Related Genes and Proteins Expression in the Follicular Membranes of Laying Ducks

XIA Weiguang¹, CHEN Wei¹, RUAN Dong¹, WANG Shuang¹, FOUAD A M^1,2, LUO Xi¹, XIONG Yunxia¹, WANG Shenglin¹, ZHENG Chuntian¹

1. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangdong Public Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. Faculty of Agriculture, Department of Animal Production, Cairo University, Giza 12613, Egypt

Received:2017-07-21 Online:2018-02-20 Published:2018-02-10

摘要/Abstract

摘要：

本试验旨在研究日粮精氨酸（Arg）水平对蛋鸭卵泡膜血管形成相关基因及蛋白表达的影响。试验选用健康、体重相近的15周龄龙岩山麻鸭396只，随机分为3组，每个组6个重复，每个重复22只，地面平养。试验蛋鸭饲喂不添加Arg的基础日粮（含0.66%精氨酸）2周后，分别饲喂在基础日粮中添加0、0.4%、0.8% L-精氨酸盐酸盐（L-Arg·HCl）的试验日粮，其Arg水平分别为0.66%、1.06%和1.46%，试验期17周。结果显示：①1.06%和1.46% Arg组试验蛋鸭血浆低密度脂蛋白-胆固醇（LDL-C）、卵巢一氧化氮（NO）浓度均显著高于0.66% Arg组（P < 0.05）。②与0.66% Arg相比，1.06%、1.46% Arg组显著上调了试验蛋鸭F2优势卵泡膜同源结构域相互作用蛋白激酶2（HIPK2）mRNA表达水平。而1.46% Arg试验蛋鸭F2优势卵泡膜血管生成素1（Ang1）mRNA表达水平显著高于0.66%、1.06% Arg组（P < 0.05）。③与0.66% Arg相比，1.06%、1.46% Arg组著上调了试验蛋鸭F2优势卵泡膜HIPK2及转化生长因子β1（TGFβ1）蛋白表达水平（P < 0.05）。综上所述，日粮1.46% Arg（在基础日粮中添加0.8% L-Arg·HCl）可通过激活TGFβ信号通路关键因子TGFβ1和HIPK2促进龙岩山麻鸭F2优势卵泡膜血管的形成，增加血流量，促进其优势卵泡的卵黄沉积。

关键词: 精氨酸; 龙岩山麻鸭; 卵泡; 卵黄沉积; 血管形成

Abstract:

This study was aimed to evaluate the effects of dietary arginine (Arg) levels on angiopoiesis related genes and proteins expression in the follicle membranes of laying ducks. 396 healthy Longyan laying ducks at 15 weeks of age with similar body weight were randomly assigned into 3 groups with 6 replicates per group and 22 birds per replicate. All laying ducks were offered a basal diet (0.66% Arg) without Arg supplementation for 2 weeks. And then were fed the experimental diets supplemented with 0,0.4% and 0.8% Arg in the form of L-arginine hydrochloride (L-Arg·HCl),which contained 0.66%,1.06% and 1.46% Arg,respectively. The experiment lasted for 17 weeks. The results showed as follows:①The LDL-C and NO concentration of laying ducks in 1.06% and 1.46% Arg groups were significantly higher than that in 0.66% Arg group (P < 0.05).②Compared to the 0.66% Arg group, the mRNA abundance of the homeodomain-interacting protein kinase 2 (HIPK2) were significantly increased in the F2 large yellow follicle membranes of laying ducks fed diets with 1.06% and 1.46% Arg (P < 0.05). The mRNA abundance of angiogenin 1 (Ang1) in the F2 large yellow follicle membranes of 1.46% Arg group was significantly higher than that in 0.66% and 1.06% Arg groups (P < 0.05).③The protein abundance of the HIPK2 and transforming growth factor β1 (TGFβ1) in the F2 large yellow follicle membranes of laying ducks fed diets with 1.06% and 1.46% Arg were significantly higher than those fed 0.66% Arg (P < 0.05). In conclusion,dietary Arg level of 1.46% that achieved by supplementing the basal diet with 0.8% L-Arg·HCl could improve angiopoiesis probably by activating HIPK2 and TGFβ1 in TGFβ signaling pathway,and increase blood flow to facilitate vitellogenesis in the large yellow follicles of Longyan laying ducks.

Key words: arginine (Arg); Longyan laying ducks; follicle; vitellogenesis; angiopoiesis

中图分类号:

Q786

夏伟光, 陈伟, 阮栋, 王爽, FOUAD A M, 罗茜, 熊云霞, 王胜林, 郑春田. 日粮精氨酸水平对蛋鸭卵泡膜血管形成相关基因及蛋白表达的影响[J]. 《中国畜牧兽医》, 2018, 45(2): 384-391.

XIA Weiguang, CHEN Wei, RUAN Dong, WANG Shuang, FOUAD A M, LUO Xi, XIONG Yunxia, WANG Shenglin, ZHENG Chuntian. Effects of Dietary Arg Levels on Angiopoiesis Related Genes and Proteins Expression in the Follicular Membranes of Laying Ducks[J]. , 2018, 45(2): 384-391.

参考文献

[1] YUAN C,LI J,DING Y,et al. Estimation of L-arginine requirement for Xinyang Black laying hens from 33 to 45 weeks of age[J].Journal of Applied Poultry Research,2015,24(4):463-469.
[2] XIA W,FOUAD A M,CHEN W,et al. Estimation of dietary arginine requirements for Longyan laying ducks[J]. Poultry Science,2017,96(1):144-150.
[3] GOEDE V,SCHMIDT T,KIMMINA S,et al. Analysis of blood vessel maturation processes during cyclic ovarian angiogenesis[J].Laboratory Investigation;A Journal of Technical Methods and Pathology, 1998,78(11):1385-1394.
[4] KIM D,LEE J,JOHNSON A L.Vascular endothelial growth factor and angiopoietins during hen ovarian follicle development[J].General and Comparative Endocrinology,2016,232:25-31.
[5] 阮栋,林映才,张罕星,等.蛋氨酸水平对开产期麻鸭产蛋性能、蛋品质及卵巢形态的影响[J].中国畜牧杂志,2012,48(7):34-37.RUAN D,LIN Y C,ZHANG H X,et al. Effect of dietary methionine level on performance,egg quality and ovarian morphology in early laying period of longyan ducks[J].Chinese Journal of Animal Science, 2012,48(7):34-37. (in Chinese)
[6] 王爽,陈伟,阮栋,等.饲粮核黄素水平对笼养蛋鸭产蛋性能、蛋品质、血液生化及抗氧化指标的影响[J].动物营养学报,2014,26(11):3284-3290.WANG S,CHEN W,RUAN D,et al. Effects of dietary riboflavin level on laying performance,egg quality, blood biochemical and antioxidant parameters of laying ducks in cage[J]. Chinese Journal of Animal Nutrition,2014,26(11):3284-3290. (in Chinese)
[7] 夏伟光,张罕星,林映才,等.饲粮代谢能和粗蛋白质水平对蛋鸭产蛋性能的影响[J].动物营养学报,2014, 26(12):3599-3607.XIA W G,ZHANG H X,LIN Y C,et al. Effects of dietary metabolizable energy and crude protein levels on laying performance of laying ducks[J]. Chinese Journal of Animal Nutrition,2014,26(12):3599-3607. (in Chinese)
[8] 张罕星,李燕,林映才,等.饲粮非植酸磷水平对高峰期蛋鸭产蛋性能、蛋品质和胫骨指标的影响[J].动物营养学报,2015,27(5):1377-1384.ZHANG H X,LI Y,LIN Y C,et al. Effects of dietary non-phytin phosphorus level on laying performance,egg quality and tibia indices of ducks during the laying peak[J]. Chinese Journal of Animal Nutrition,2015, 27(5):1377-1384. (in Chinese)
[9] CHEN W,ZHANG H X,WANG S,et al. Estimation of dietary selenium requirement for Chinese egg-laying ducks[J].Animal Production Science,2015,55(8):1056-1063.
[10] XIA W G,ZHANG H X,LIN Y C,et al. Evaluation of dietary calcium requirements for laying Longyan shelducks[J]. Poultry Science,2015,94(12):2932-2937.
[11] FOUAD A M,CHEN W, RUAN D, et al. Effects of dietary lysine supplementation on performance,egg quality,and development of reproductive system in egg-laying ducks[J]. Journal of Applied Animal Research,2017,Epub of ahead.
[12] FOUAD A M,RUAN D,LIN Y C,et al. Effects of dietary methionine on performance,egg quality and glutathione redox system in egg-laying ducks[J].British Poultry Science,2016,57(6):818-823.
[13] LIVAK K J,SCHMITTGEN T D. Analysis of relative gene expression data using Real-time quantitative PCR and the 2(-Delta Delta C(T)) method[J].Methods,2001, 25(4):402-408.
[14] KELLEY D,LEBLANC M M,WARREN L K,et al. Influence of L-arginine supplementation on reproductive blood flow and embryo recovery rates in mares[J].Theriogenology,2014,81(5):752-757.
[15] ANTEBY E Y,HURWITZ A,KORACH O,et al. Human follicular nitric oxide pathway:Relationship to follicular size,oestradiol concentrations and ovarian blood flow[J].Human Reproduction,1996,11(9):1947-1951.
[16] BASINI G,GRASSELLI F.Nitric oxide in follicle development and oocyte competence[J]. Reproduction, 2015,150(1):1-9.
[17] MANWAR S J,MOUDGAL R P,SASTRY K V,et al. Role of nitric oxide in ovarian follicular development and egg production in Japanese quail (Coturnixcoturnix japonica)[J]. Theriogenology,2006,65(7):1392-1400.
[18] SILVA L A,GASTAL E L,GASTAL M O,et al. Relationship between vascularity of the preovulatory follicle and establishment of pregnancy in mares[J].Animal Reproduction,2006,3(3):339-346.
[19] MORTENSEN C J,KELLEY D E,WARREN L K. Supplemental L-arginine shortens gestation length and increases mare uterine blood flow before and after parturition[J].Journal of Equine Veterinary Science,2011,31(9):514-520.
[20] MUROHARA T,ASAHARA T,SILVER M,et al. Nitric oxide synthase modulates angiogenesis in response to tissue ischemia[J].Journal of Clinical Investigation,1998,101(11):2567-2578.
[21] PINTO C R,PACCAMONTI D L,EILTS B E,et al. Concentrations of nitric oxide in equine preovulatory follicles before and after administration of human chorionic gonadotropin[J].Theriogenology,2003, 60(5):819-827.
[22] SEEKALLU S V,TOOSI B M,GRAZUL-BILSKA A T,et al. Markers of ovarian antral follicular development in sheep:Comparison of follicles destined to ovulate from the final or penultimate follicular wave of the estrous cycle[J].Reproduction,2010,140(4):559-568.
[23] TESSARO I,LUCIANO A M,FRANCIOSI F,et al. The endothelial nitric oxide synthase/nitric oxide system is involved in the defective quality of bovine oocytes from low mid-antral follicle count ovaries[J]. Journal of Animal Science,2011,89(8):2389-2396.
[24] SHANG Y,DOAN C N,ARNOLD T D,et al. Transcriptional corepressors HIPK1 and HIPK2 control angiogenesis via TGF-beta-TAK1-dependent mechanism[J].PLoS Biology,2013,11(4):e1001527.
[25] PUCA R,NARDINOCCHI L,D'ORAZI G. Regulation of vascular endothelial growth factor expression by homeodomain-interacting protein kinase-2[J]. Journal of Experimental & Clinical Cancer Research, 2008,27:22.
[26] AIKAWA Y,NGUYENL A,ISONO K,et al.Roles of HIPK1 and HIPK2 in AML1-and p300-dependent transcription, hematopoiesis and blood vessel formation[J].The Embo Journal,2006,25(17):3955-3965.

日粮精氨酸水平对蛋鸭卵泡膜血管形成相关基因及蛋白表达的影响

Effects of Dietary Arg Levels on Angiopoiesis Related Genes and Proteins Expression in the Follicular Membranes of Laying Ducks

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