转化生长因子βⅠ型受体基因在敖汉细毛羊不同组织及发情时期卵巢中的表达分析

doi:10.16431/j.cnki.1671-7236.2017.07.006

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (7): 1941-1946.doi: 10.16431/j.cnki.1671-7236.2017.07.006

转化生长因子βⅠ型受体基因在敖汉细毛羊不同组织及发情时期卵巢中的表达分析

于顺雨¹, 刘开东², 栾兆进³, 王国义⁴, 柳楠¹, 贺建宁¹

1. 青岛农业大学动物科技学院, 青岛 266109;
2. 青岛畜牧兽医研究所, 青岛 266121;
3. 包头市农牧业科学研究所, 包头 014013;
4. 赤峰市敖汉旗种羊场, 赤峰 024300

收稿日期:2017-01-05 出版日期:2017-07-20 发布日期:2017-07-22
通讯作者: 贺建宁 E-mail:hexingxing104@163.com
作者简介:于顺雨(1991-),男,山东烟台人,硕士生,研究方向:分子遗传与动物遗传育种,E-mail:985352431@qq.com
基金资助:
国家自然科学基金项目（31402047）；国家绒毛用羊产业技术体系专项资金（CARS-40）；青岛农业大学高层次人才科研基金（631410）

Expression Analysis of TGF-βRⅠ Gene in Ovary of AoHan Fine-wool Sheep at Different Tissues and Estrous Cycles

YU Shun-yu¹, LIU Kai-dong², LUAN Zhao-jin³, WANG Guo-yi⁴, LIU Nan¹, HE Jian-ning¹

1. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China;
2. Qingdao Institute of Animal Husbangdry and Veterinary, Qingdao 266121, China;
3. Baotou Agricultural and Animal Husbandry Science Institute, Baotou 014013, China;
4. Aohan Banner in Chifeng, Chifeng 024300, China

Received:2017-01-05 Online:2017-07-20 Published:2017-07-22

摘要/Abstract

摘要：

试验旨在探究转化生长因子βⅠ型受体（transforming growth factor-beta receptorⅠ，TGF-βRⅠ）基因在敖汉细毛羊中的组织表达情况，以及在不同发情时期卵巢中表达量的变化规律，为探讨其与绵羊发情的关系奠定基础。以24只季节性发情的敖汉细毛羊为研究对象，分为乏情期、发情间期、发情前期和发情期4组，每组6只。首先利用实时荧光定量PCR检测其发情期甲状腺、下丘脑、垂体、卵巢、肾上腺、心脏、肝脏、脾脏、肺脏、肾脏、胰腺、肌肉12个组织中TGF-βRⅠ基因的表达情况，其次对4个不同发情时期卵巢TGF-βRⅠ基因的表达量变化进行研究。结果显示，TGF-βR Ⅰ在各组织中均有表达，在卵巢和甲状腺中表达量最高，极显著高于其他组织（P<0.01）；在下丘脑、垂体、胰腺、肾上腺、脾脏和肺脏组织表达量较高，显著高于心脏、肝脏、肾脏和肌肉组织（P<0.05）。卵巢中TGF-βRⅠ基因在发情前期表达量最高，极显著高于其他3个时期（P<0.01），发情间期表达量最低，极显著低于其他3个时期（P<0.01）。综上所述，卵巢组织中TGF-βRⅠ基因在发情前期时可能对排卵前卵泡的成熟起促进作用。

关键词: 敖汉细毛羊; TGF-β RⅠ基因; 卵巢; 季节性发情

Abstract:

This study was aimed to investigate the expression of transforming growth factor-beta receptor Ⅰ(TGF-βR Ⅰ) in main tissue of AoHan Fine-wool sheep,the expression variations of ovary in different estrous states,and the mechanism of TGF-βRⅠ gene regulation in seasonal estrus of AoHan Fine-wool sheep. Twenty-four seasonal estrus AoHan Fine-wool sheep were chosen and divided into 4 groups of anestrus, proestrus, estrus and dioestrum with 6 sheep per group. First of all,the expression of TGF-βRⅠ gene in twelve tissues (thyroid,hypothalamus,hypophysis,ovary,adrenal gland,heart,liver,spleen,lung,kidney,pancreas and muscle) of AoHan Fine-wool sheep in the estrous stage and its expression variations in ovary of anestrus,proestrus,estrus and dioestrum were detected by Real-time PCR. The results showed that TGF-βRⅠ gene expressed in all the 12 tissues,and its expression in ovary and thyroid were extremely significantly higher than that in others (P<0.01). The expression of TGF-βRⅠ gene in hypothalamus,hypophysis,pancreas,adrenal gland, spleen and lung were high,which were significant higher than heart,liver,kidney and muscle (P<0.05).The expression levels of TGF-βRⅠgene in proestrus were extremely higher than other stages (P<0.01). The expression in dioestrum were the lowest which extremely lower than other stages (P<0.01). The above results indicated that TGF-βRⅠ might play an important role in turning on the maturation of follice.

Key words: AoHan Fine-wool sheep; TGF-βRⅠgene; ovary; seasonal estrus

中图分类号:

S826.9⁺1

于顺雨, 刘开东, 栾兆进, 王国义, 柳楠, 贺建宁. 转化生长因子βⅠ型受体基因在敖汉细毛羊不同组织及发情时期卵巢中的表达分析[J]. 《中国畜牧兽医》, 2017, 44(7): 1941-1946.

YU Shun-yu, LIU Kai-dong, LUAN Zhao-jin, WANG Guo-yi, LIU Nan, HE Jian-ning. Expression Analysis of TGF-βRⅠ Gene in Ovary of AoHan Fine-wool Sheep at Different Tissues and Estrous Cycles[J]. , 2017, 44(7): 1941-1946.

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转化生长因子βⅠ型受体基因在敖汉细毛羊不同组织及发情时期卵巢中的表达分析

Expression Analysis of TGF-βRⅠ Gene in Ovary of AoHan Fine-wool Sheep at Different Tissues and Estrous Cycles

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