奶牛饲料中酿酒酵母菌的分离鉴定及其发酵液的体外抑菌活性研究

doi:10.16431/j.cnki.1671-7236.2016.05.016

›› 2016, Vol. 43 ›› Issue (5): 1226-1231.doi: 10.16431/j.cnki.1671-7236.2016.05.016

奶牛饲料中酿酒酵母菌的分离鉴定及其发酵液的体外抑菌活性研究

刘龙海¹, 李新圃¹, 杨峰¹, 罗金印¹, 张哲^1,2, 李宏胜¹

1. 中国农业科学院兰州畜牧与兽药研究所, 农业部兽用药物创制重点实验室, 甘肃省新兽药工程重点实验室, 兰州 730050;
2. 甘肃农业大学动物医学院, 兰州 730070

收稿日期:2015-11-23 出版日期:2016-05-20 发布日期:2016-05-23
通讯作者: 李宏胜 E-mail:myslhs@163.com
作者简介:刘龙海(1990-),男,山东临沂人,硕士,研究方向:奶牛乳房炎疫苗,E-mail:llh20103549@163.com
基金资助:
"十二五"国家科技支撑计划(2012BAD12B03);甘肃省科技支撑计划(144NKCA240);甘肃省农业科技创新项目(GNCX-2013-59);中国农业科学院奶牛疾病创新工程(CAAS-ASTIP-2014-LIHPS-03)

The Study of Isolation, Identification of S.cerevisiae from Dairy Feed and its Antibacterical Activity in the Fermentation Brothe in vitro

LIU Long-hai¹, LI Xin-pu¹, YANG Feng¹, LUO Jin-yin¹, ZHANG Zhe^1,2, LI Hong-sheng¹

1. Key Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Discovery, Ministry of Agriculture, Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China;
2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

Received:2015-11-23 Online:2016-05-20 Published:2016-05-23

摘要/Abstract

摘要： 本试验旨在分离鉴定甘肃地区奶牛场饲料中的酿酒酵母菌,进而研究酿酒酵母菌发酵液的体外抑菌活性。通过YPD固体培养基对采集的5份饲草和5份饲料样品中的酵母菌进行分离,分别进行生化鉴定和26SrDNA测序鉴定,进而检测酿酒酵母发酵液的体外抑菌活性。结果表明,从样品中分离出4种共12株酵母菌,分别为3株酿酒酵母菌(Y1、Y7和Y11)、4株汉逊德巴利酵母菌、3株浅黄隐球酵母菌和2株戴尔有孢圆酵母菌;3株酿酒酵母菌的发酵液对奶牛乳房炎金黄色葡萄球菌、大肠杆菌和无乳链球菌的抑菌活性存在差异,其中酵母菌Y7和Y11的发酵液对3种病原菌均具有抑菌活性,而Y1的发酵液只对大肠杆菌具有抑制作用。本试验为进一步筛选性能优异的酿酒酵母菌用于微生态制剂研发奠定了基础。

关键词: 酿酒酵母菌; 奶牛饲料; 分离鉴定; 抑菌活性

Abstract: The aim of the study was to isolate and identify S.cerevisiae in the dairy feed and analyze antibacterial activity of S.cerevisiae fermentation brothe in vitro.5 forage samples and 5 feed samples were collected to isolate yeast by YPD solid medium.Biochemical identification and 26S rDNA sequence were used to identify the isolated strains.The antibacterial activity of S.cerevisiae strains was tested by cylinder plate method.A total of 12 yeast belonged to 4 species were isolated and identified, including 3 S.cerevisiae (Y1, Y7 and Y11), 4 Debaryomyces hansenii, 3 Cryptococcus flavescens and 2 Torulaspora debrueckii.The antibacterial activities of the 3 S.cerevisiae fermentation brothe to Staphylococcus aureus, E.coli and Streptococcus agalactiae were different.Y7 and Y11 fermentation brothe showed antibacterial activities to all the tested pathogens, while Y1 inhibit E.coli alone.This study laid the foundations for further research of probiotics containing S.cerevisiae with excellent performance.

Key words: S.cerevisiae; cows feed; isolation and identification; antibacterical activity

中图分类号:

S816

刘龙海, 李新圃, 杨峰, 罗金印, 张哲, 李宏胜. 奶牛饲料中酿酒酵母菌的分离鉴定及其发酵液的体外抑菌活性研究[J]. , 2016, 43(5): 1226-1231.

LIU Long-hai, LI Xin-pu, YANG Feng, LUO Jin-yin, ZHANG Zhe, LI Hong-sheng. The Study of Isolation, Identification of S.cerevisiae from Dairy Feed and its Antibacterical Activity in the Fermentation Brothe in vitro[J]. , 2016, 43(5): 1226-1231.

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奶牛饲料中酿酒酵母菌的分离鉴定及其发酵液的体外抑菌活性研究

The Study of Isolation, Identification of S.cerevisiae from Dairy Feed and its Antibacterical Activity in the Fermentation Brothe in vitro

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