利用Solexa测序技术鉴定策勒黑羊microRNA

doi:10.16431/j.cnki.1671-7236.2016.01.030

›› 2016, Vol. 43 ›› Issue (1): 204-209.doi: 10.16431/j.cnki.1671-7236.2016.01.030

利用Solexa测序技术鉴定策勒黑羊microRNA

曾献存¹, 贾斌¹, 史洪才², 申红¹, 张永生¹, 陈韩英³

1. 石河子大学动物科技学院, 石河子 832003;
2. 新疆畜牧科学院农业部家畜繁育重点开发实验室, 乌鲁木齐 832000;
3. 石河子大学药学院, 石河子 832003

收稿日期:2015-06-30 出版日期:2016-01-20 发布日期:2016-01-23
通讯作者: 陈韩英 E-mail:56355633@qq.com
作者简介:曾献存(1981-),男,安徽太和人,博士,研究方向:动物遗传育种,E-mail:zengxiancun@163.com
基金资助:
国家自然科学基金项目(31260536);石河子大学高层次人才启动项目(RCZX201202)

microRNA Identification of Ovaries in Qira Black Sheep by Solexa Sequencing

ZENG Xian-cun¹, JIA Bin¹, SHI Hong-cai², SHEN Hong¹, ZHANG Yong-sheng¹, CHEN Han-ying³

1. College of Animal Science and Technology, Shihezi University, Shihezi 832003, China;
2. Key Laboratory of Ministry for Biotechnology, Xinjiang Academy of Animal Science, Urumqi 832000, China;
3. School of Pharmacy, Shihezi University, Shihezi 832003, China

Received:2015-06-30 Online:2016-01-20 Published:2016-01-23

摘要/Abstract

摘要： 试验旨在挖掘、分析策勒黑羊卵巢组织microRNA(miRNA),为进一步研究特定miRNA参与策勒黑羊卵泡发育及激素分泌等相关生殖活动的机制奠定理论基础。从策勒黑羊卵巢组织中提取总RNA,分离、构建小片段RNA文库,进行Solexa测序和生物信息学分析。结果表明,共获得9527311条干净序列读数,小RNA序列长度主要分布在21~23 nt;共鉴定出434个策勒黑羊卵巢组织表达的保守miRNA,其中表达量在100以上有167个;鉴定出57个候选miRNA,其中表达量在100以上有8个。测序成功获取了策勒黑羊卵巢组织miRNA序列及表达谱,卵巢组织miRNA表达丰富且表达量各异。

关键词: miRNA; Solexa; 卵巢; 策勒黑羊

Abstract: With the purposes of providing basic information for further studies of Qira Black sheep reproduction activities such as folliculogenesis and hormone secretion, characterization of microRNA(miRNA) expression in Qira Black sheep ovary tissues was studied.In this study, the small RNA isolated from total RNA of Qira Black sheep ovary tissues were sequenced by Solexa and then bioinformatics analysis were performed.The results showed that 9527311 clean reads were obtained, and the 21-23 nt small RNA was the major RNA in the total sequences.434 ovary conservative miRNA and 57 new miRNA were identified.Among them, there were 167 conservative including 8 new miRNA exceeded 100 in the expression levels.The study succeeded to construct the expression library of miRNA which were abundant and differentially expressed in Qira Black sheep ovary tissues.

Key words: miRNA; Solexa; ovary; Qira Black sheep

中图分类号:

曾献存, 贾斌, 史洪才, 申红, 张永生, 陈韩英. 利用Solexa测序技术鉴定策勒黑羊microRNA[J]. , 2016, 43(1): 204-209.

ZENG Xian-cun, JIA Bin, SHI Hong-cai, SHEN Hong, ZHANG Yong-sheng, CHEN Han-ying. microRNA Identification of Ovaries in Qira Black Sheep by Solexa Sequencing[J]. , 2016, 43(1): 204-209.

参考文献

[1] Kim V N.microRNA biogenesis:Coordinated cropping and dicing[J]. Nat Rev Mol Cell Biol,2005,6(5):376-385.
[2] Song L,Tuan R S.microRNAs and cell differentiation in mammalian development[J]. Birth Defects Res C Embryo Today,2006,78(2):140-149.
[3] Ambros V.The functions of animal microRNAs[J]. Nature,2004,431:350-355.
[4] Plasterk R H.microRNAs in animal development[J]. Cell, 2006,124:877-881.
[5] Kloosterman W P,Plasterk R H.The diverse functions of microRNAs in animal development and disease[J]. Dev Cell,2006,11:441-450.
[6] Baley J,Li J.microRNAs and ovarian function[J]. J Ovarian Res,2012,5:8.
[7] Hossain M M,Sohel M M H,Schellander K,et al.Characterization and importance of microRNAs in mammalian gonadal functions[J]. Cell Tissue Res,2012,349:679-690.
[8] Donadeu F X,Schauer S N,Sontakke S D.Involvement of miRNAs in ovarian follicular and luteal development[J]. J Endocrinol,2012,215:323-334.
[9] McBride D,Carre W,Sontakke S D,et al.Identification of miRNAs associated with the follicular-luteal transition in the ruminant ovary[J]. Reproduction,2012,144:221-233.
[10] Hossain M M,Ghanem N,Hoelker M,et al.Identification and characterization of miRNAs expressed in the bovine ovary[J]. BMC Genomics,2009,10:443.
[11] 张晓东,凌英会,张运海,等.应用Solexa测序技术挖掘山羊卵巢组织microRNA[J].中国农业科学,2013,46(1):146-153.
[12] Zhang X D,Zhang Y H,Ling Y H,et al.Characterization and differential expression of microRNAs in the ovaries of pregnant and non-pregnant goats(Capra hircus)[J]. BMC Genomics,2013,14:157.
[13] 常卫华.成年高山细毛羊卵巢和睾丸miRNA的筛选鉴定[D].兰州:甘肃农业大学,2014.
[14] Ro S,Song R,Park C,et al.Cloning and expression profiling of small RNAs expressed in the mouse ovary[J]. RNA,2007,13(12):2366-2380.
[15] Roush S,Slack F J.The let-7 family of microRNAs[J]. Trends in Cell Biology,2008,18(10):505-516.
[16] Miles J R,McDaneld T G,Wiedmann R T,et al.microRNA expression profile in bovine cumulus-oocyte complexes:Possible role of let-7 and miR-106a in the development of bovine oocytes[J]. Animal Reproduction Science,2012,130(1/2):16-26.
[17] Hwang H W,Mendell J T.microRNAs in cell proliferation,cell death,and tumorigenesis[J]. Br J Cancer,2007,96(Suppl):R40-R44.
[18] Yao N,Yang B Q,Liu Y,et al.Follicle-stimulating hormone regulation of microRNA expression on progesterone production in cultured rat granulosa cells[J]. Endocrine,2010,38(2):158-166.
[19] Carletti M Z,Fiedler S D,Christenson L K.microRNA 21 blocks apoptosis in mouse periovulatory granulosa cells[J]. Biology of Reproduction,2010,83(2):286-295.

利用Solexa测序技术鉴定策勒黑羊microRNA

microRNA Identification of Ovaries in Qira Black Sheep by Solexa Sequencing

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	胡文, 霍孔林, 宋星星, 张鑫, 张多妮, 罗荣荣, 徐文镐, 李珣. 促性腺激素抑制激素对SD大鼠性腺生殖功能与糖代谢的影响[J]. 中国畜牧兽医, 2022, 49(6): 2239-2249.
[2]	张继虎, 米热妮萨, 王子渲, 李琳, 邢凤, 刘书东. 策勒黑羊ERα基因克隆、生物信息学分析及卵巢组织表达研究[J]. 中国畜牧兽医, 2022, 49(5): 1679-1687.
[3]	王东, 梁加越, 王笑冰, 冯娟, 张茹, 夏慧岩, 肖红梅. 绒山羊毛囊发育中非编码RNA的研究进展[J]. 中国畜牧兽医, 2022, 49(5): 1817-1827.
[4]	张育铭, 陈乔光, 候照峰, 刘丹丹, 陶建平, 许金俊. 病原感染后鸡miRNAs调控的研究进展[J]. 中国畜牧兽医, 2022, 49(5): 1908-1916.
[5]	石雨竹, 罗儒唐, 陈超, 吴旭, 李昂. 番鸭GDF9基因克隆、生物信息学及组织表达分析[J]. 中国畜牧兽医, 2022, 49(3): 817-829.
[6]	王月丽, 邵志然, 易继海, 王勇, 王震, 陈创夫. miR-145a-3p通过靶向ATG14调控布鲁氏菌诱导的RAW264.7自噬[J]. 中国畜牧兽医, 2022, 49(3): 1117-1125.
[7]	翁钰, 王琴, 赵玉芬, 于凯, 郝绍瑜, 于泊洋, 杜晨光, 苏布登格日勒, 李海军. DHEA诱导小鼠PCOS模型过程中性激素的变化规律[J]. 中国畜牧兽医, 2022, 49(2): 454-461.
[8]	黄晓刚, 韩贝贝, 李菊, 张守全. miRNA在哺乳动物配子发生中的作用研究进展[J]. 中国畜牧兽医, 2022, 49(2): 624-630.
[9]	翟亚莹, 施巧婷, 楚秋霞, 朱肖亭, 滑留帅, 张子敬, 陈付英, 祁兴磊, 王二耀, 吕世杰. 胚胎移植前供体牛与受体牛血浆外泌体miRNA差异表达分析[J]. 中国畜牧兽医, 2022, 49(2): 641-649.
[10]	赵晓钰, 吴盈萍, 李海英, 姚莹莹, 李家辉, 姚洋, 卢庆庆. 伊犁鹅产蛋前后各期卵巢转录谱的构建及卵泡发育相关基因的分析[J]. 中国畜牧兽医, 2022, 49(12): 4674-4687.
[11]	侯俊杰, 史卓言, 刘小平, 冀祥, 楚潇然, 宋祯, 文凤云. microRNA调控猪肌内脂肪沉积的研究进展[J]. 中国畜牧兽医, 2022, 49(11): 4318-4326.
[12]	董书餐, 侯碧巍, 邹娴, 李耀坤, 刘德武, 孙宝丽, 郭勇庆, 邓铭, 柳广斌. 细胞自噬对动物卵泡发育调控的研究进展[J]. 中国畜牧兽医, 2022, 49(11): 4335-4345.
[13]	禄欢, 牛熙, 黄娅丽, 李升, 冉雪琴, 黄世会, 王嘉福. 调控香猪繁殖候选miRNA筛选[J]. 中国畜牧兽医, 2021, 48(9): 3118-3127.
[14]	喻世刚, 王钢, 廖娟, 谢伟. 沐川乌骨黑鸡肌内脂肪沉积相关microRNAs的筛查与鉴定[J]. 中国畜牧兽医, 2021, 48(8): 2713-2726.
[15]	任巧玲, 张家庆, 郭红霞, 王献伟, 王璟, 吕玲燕, 陈俊峰, 马强, 张彬, 邢宝松, 徐泽君. 发情和乏情初产母猪下丘脑-垂体-卵巢轴差异表达基因筛选及分析[J]. 中国畜牧兽医, 2021, 48(8): 2864-2877.