BRCA1基因多态性及其与奶牛乳房炎抗性的相关性研究

doi:10.16431/j.cnki.1671-7236.2015.12.031

›› 2015, Vol. 42 ›› Issue (12): 3323-3328.doi: 10.16431/j.cnki.1671-7236.2015.12.031

BRCA1基因多态性及其与奶牛乳房炎抗性的相关性研究

杨宇泽¹, 冯涛², 唐韶青¹, 章玉涛³, 靳月生⁴, 王艳⁵, 赵春颖¹, 路永强¹

1. 北京市畜牧总站, 北京 100107;
2. 北京市农林科学院畜牧兽医研究所, 北京 100097, ;
3. 北京中地畜牧科技有限公司, 北京 101308;
4. 北京市昌平区农业局, 北京 102200;
5. 北京市大兴区动物疾病控制中心, 北京 102600

收稿日期:2015-05-21 出版日期:2015-12-20 发布日期:2015-12-30
通讯作者: 路永强 E-mail:luyongqiang@163.com
作者简介:杨宇泽(1982-),男,山西朔州人,硕士,高级畜牧师,研究方向:动物遗传育种与繁殖冯涛(1980-),男,甘肃庆阳人,博士,助理研究员,研究方向:动物繁殖,E-mail:fengtao_gs@163.com
基金资助:
北京市农业局科技新星计划项目(20130906);北京市优秀人才培养资助(2014000020060G181)

Polymorphism of BRCA1 Gene and its Association with Mastitis Resistance in Holstein Dairy Cows

YANG Yu-ze¹, FENG Tao², TANG Shao-qing¹, ZHANG Yu-tao³, JIN Yue-sheng⁴, WANG Yan⁵, ZHAO Chun-ying¹, LU Yong-qiang¹

1. Beijing General Station of Animal Husbandry Service, Beijing 100107, China;
2. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
3. Beijing Sino-farm Animal Husbandry Technology Co., Ltd., Beijing 101308, China;
4. Changping Municipal Bureau of Agriculture in Beijing City, Beijing 102200, China;
5. Daxing Center for Animal Disease Control and Prevention in Beijing City, Beijing 102600, China

Received:2015-05-21 Online:2015-12-20 Published:2015-12-30

摘要/Abstract

摘要： 为了研究BRCA1基因突变与荷斯坦奶牛体细胞数和体细胞评分的关系,试验通过对BRCA1基因外显子13、14进行克隆、序列比对和挖掘已有突变的方法确定该基因的多态位点,采用SNaPshot技术检测了BRCA1基因25025 T>A和46126 G>T突变位点在北京郊区荷斯坦奶牛群体中的分布,并对突变位点与体细胞数和体细胞评分进行了关联分析。结果表明,荷斯坦奶牛BRCA1基因2个位点均检测到3种基因型,其中25025 bp位点TT基因型为优势基因型,46126 bp位点GT基因型为优势基因型。25025 bp位点AA基因型个体体细胞数(P<0.05)和体细胞评分(P<0.01)都显著低于TT和TA基因型;46126 bp位点TT基因型个体体细胞数显著低于GG和GT基因型个体(P<0.05),但3种基因型个体体细胞评分无显著差异(P>0.05)。本研究结果初步表明,BRCA1基因25025和46126 bp位点可作为中国荷斯坦牛乳房炎抗性的标记辅助选择。

关键词: 荷斯坦奶牛; BRCA1基因; 体细胞数; 体细胞评分

Abstract: In order to study mutations of bovine breast cancer 1 (BRCA1) gene and its association with somatic cell count and somatic cell score in Holstein dairy cows in Beijing.SNPs of BRCA1 gene exon 13 and 14 were obtained by cloning,sequence alignment and searching for known mutations.In our research,25025 T>A of exon 9 and 46126 G>T of exon 13 in BRCA1 were detected by SNaPshot in Holstein dairy cows raised on the outskirts of Beijing city,as well as the association between SNPs and somatic cell count (SCC) or somatic cell score (SCS) were also analysed.The results showed that both 25025 T>A and 46126 G>T were detected in Holstein dairy cows.TT genotype at locus 25025 T>A and GT genotype at locus 46126 G>T were dominant genotypes,respectively.At locus 25025 T>A,least squares mean of SCC and SCS in individuals with AA genotype was significantly lower than that with TT and TA genotypes (P<0.05;P<0.01).Least squares mean of SCC in individuals with TT genotype was significantly lower than that with GG and GT genotypes (P<0.05),while SCS of different genotypes showed no difference at locus 46126 G>T (P>0.05).These results preliminarily indicated that the 25025 T>A and 46126 G>T mutations of BRCA1 gene could be used in molecular marker-assisted selection programs of mastitis resistance in Holstein dairy cows.

Key words: Holstein dairy cows; BRCA1 gene; somatic cell count; somatic cell score

中图分类号:

S823.9⁺1

杨宇泽, 冯涛, 唐韶青, 章玉涛, 靳月生, 王艳, 赵春颖, 路永强. BRCA1基因多态性及其与奶牛乳房炎抗性的相关性研究[J]. , 2015, 42(12): 3323-3328.

YANG Yu-ze, FENG Tao, TANG Shao-qing, ZHANG Yu-tao, JIN Yue-sheng, WANG Yan, ZHAO Chun-ying, LU Yong-qiang. Polymorphism of BRCA1 Gene and its Association with Mastitis Resistance in Holstein Dairy Cows[J]. , 2015, 42(12): 3323-3328.

参考文献

[1] Chu M X,Ye S C,Qiao L,et al.Polymorphism of exon 2 of BoLADRB3 gene and its relationship with somatic cell score in Beijing Holstein cows[J].Mol Biol Rep,2012,39(3):2909-2914.
[2] Rupp R,Boichard D.Genetic parameters for clinical mastitis,somatic cell score,production,udder type traits,and milking ease in first lactation Holsteins[J].J Dairy Sci,1999,82(10):2198-2204.
[3] Heringstad B,Gianola D,Chang Y M,et al.Genetic associations between clinical mastitis and somatic cell score in early first-lactation cows[J].J Dairy Sci,2006,89(6):2236-2244.
[4] Shook G E.Major advances in determining appropriate selection goals[J].J Dairy Sci,2006,89(4):1349-1361.
[5] 陈仁金,王珍珍,杨章平,等.中国荷斯坦牛Toll样受体4基因的遗传多态性与体细胞评分的关联分析[J].中国畜牧兽医,2013,40(11):134-138.
[6] Miki Y,Swensen J,Shattuck-Eidens D,et al.A strong candidate for the breast and ovarian cancer susceptibilitygene BRCA1[J].Science,1994,266(5182):66-71.
[7] Xu J,Wang B,Zhang Y,et al.Clinical implications for BRCA gene mutation in breast cancer[J].Mol Biol Rep,2012,39(3):3097-3102.
[8] Krum S A,Womack J E,Lane T F.Bovine BRCA1 shows classic responses to genotoxic stress but low in vitro transcriptional activation activity[J].Oncogene,2003,22(38):6032-6044.
[9] Mahfoudh W,Bouaouina N,Ahmed S B,et al.Hereditary breast cancer in Middle Eastern and North African (MENA) populations:Identification of novel,recurrent and founder BRCA1 mutations in the Tunisian population[J].Mol Biol Rep,2012,39(2):1037-1046.
[10] Rebbeck T R.Inherited genetic predisposition in breast cancer.A populationbased perspective[J].Cancer,1999,86(11):2493-2501.
[11] Kennedy R D,Quinn J E,Johnston P G,et al.BRCA1:Mechanisms of inactivation and implications for management of patients[J].Lancet,2002,360(9338):1007-1014.
[12] Bennewitz J,Reinsch N,Guiard V,et al.Multiple quantitative trait loci mapping with cofactors and application of alternative variants of the false discovery rate in an enlarged granddaughter design[J].Genetics,2004,168(2):1019-1027.
[13] Daetwyler H D,Schenkel F S,Sargolzaei M,et al.A genome scan to detect quantitative trait loci for economically important traits in Holstein cattle using two methods and a dense single nucleotide polymorphism map[J].J Dairy Sci,2008,91(8):3225-3236.
[14] Yuan Z,Chu G,Dan Y,et al.BRCA1:A new candidate gene for bovine mastitis and its association analysis between single nucleotide polymorphisms and milk somatic cell score[J]. Mol Biol Rep,2012,39(6):6625-6631.
[15] Yuan Z,Li J,Li J,et al.Investigation on BRCA1 SNPs and its effects on mastitis in Chinese commercial cattle[J].Gene,2012,505(1):190-194.

BRCA1基因多态性及其与奶牛乳房炎抗性的相关性研究

Polymorphism of BRCA1 Gene and its Association with Mastitis Resistance in Holstein Dairy Cows

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