华中地区猪繁殖与呼吸综合征病毒临床流行毒株Nsp2和GP5氨基酸的遗传变异分析

doi:10.16431/j.cnki.1671-7236.2015.11.030

›› 2015, Vol. 42 ›› Issue (11): 3026-3036.doi: 10.16431/j.cnki.1671-7236.2015.11.030

华中地区猪繁殖与呼吸综合征病毒临床流行毒株Nsp2和GP5氨基酸的遗传变异分析

李彬¹, 王豪男¹, 彭忠², 胡睿铭², 吴斌^1,2

1. 华中农业大学农业微生物学国家重点实验室, 武汉 430070;
2. 生猪健康养殖协同创新中心, 武汉 430070

收稿日期:2015-06-26 出版日期:2015-11-20 发布日期:2015-11-26
通讯作者: 吴斌 E-mail:wub@mail.hzau.edu.cn
作者简介:李彬(1990-),男,河南开封人,硕士生,研究方向:动物传染病,E-mail:vet90li@hotmail.com
基金资助:
湖北省科技支撑计划优质家畜新品种(系)培育及高效安全生产关键技术(2013BBB14);国家科技支撑计划项目优良种猪高效利用关键工程化技术集成与示范(2014BAD20B00)

Genetic Variation Analysis of Nsp2 and GP5 Amino Acids of Porcine Reproductive and Respiratory Syndrome Virus in Clinical in Central China

LI Bin¹, WANG Hao-nan¹, PENG Zhong², HU Rui-ming², WU Bin^1,2

1. State Key Laboratory of Agricultural Microbiology of Huazhong Agricultural University, Wuhan 430070, China;
2. Cooperative Innovation Centerfor Sustainable Pig Production, Wuhan 430070, China

Received:2015-06-26 Online:2015-11-20 Published:2015-11-26

摘要/Abstract

摘要： 为了解当前猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)流行毒株的遗传变异情况,本研究对2013-2014年间来源于中国华中地区湖北、河南和湖南3省20个猪场65份疑似PRRSV感染的临床样品开展病毒分离工作,通过RT-PCR及间接免疫荧光试验进行鉴定,结果显示成功分离到4株PRRSV。通过测序获得它们的部分Nsp2氨基酸序列,经比对分析,确定分离毒株皆为变异毒株,并且与JXA1、TJ等高致病性毒株有相同的遗传特点,在Nsp2区域有30个氨基酸的不连续缺失。本研究将从临床样品中测序获得的23株PRRSV的GP5氨基酸序列与GenBank公布的有代表性的毒株序列进行遗传进化分析,结果显示所有毒株均为美洲型毒株,其中21株与高致病性变异毒株(JXA1、TJ和HUN4)同源性较高,同时在主要中和位点和N-糖基化位点存在部分氨基酸突变,另外两株与疫苗毒株RespPRRS MLV同源性较高。本试验结果表明,当前高致病性PRRSV依然是优势毒株,其GP5氨基酸突变频繁,不同地区流行毒株存在一定的遗传差异。

关键词: 猪繁殖与呼吸综合征; 分离鉴定; 序列分析

Abstract: In order to disclose the genetic variation of PRRSV,65 materials from some areas were identified during the period from 2013 to 2014.Viruses were isolated from the suspected PRRS samples.Four isolates were isolated and identified by RT-PCR and IFA.Partial Nsp2 amino acid sequences of the isolates were obtained by sequencing,and comparison analysis showed that all of the isolates were variant strains.Then we found a discontinuous deletion of 30 amino acids in Nsp2.It shared the same feature with JXA1 and TJ.Besides,we sequenced and analyzed the GP5 amino acids of 23 currently epidemic strains with some typical strains published online earlier,the results showed that all strains were American strain,21 of which had high homology with highly pathogenic variant strains (JXA1,TJ and HUN4).Moreover,further analysis of the GP5 protein suggested that these strains exhibited variations in the primary neutralizing epitope and N-linked glycosylation sites.The other two strains had the same genetic features with RespPRRSV MLV.The results showed that the highly pathogenic PRRSV was still the dominant strain and GP5 amino acid mutations were frequently,there were some genetic differences between PRRSV in different regions.

Key words: porcine reproductive and respiratory syndrome; isolation and identification; sequence analysis

中图分类号:

S852.65

李彬, 王豪男, 彭忠, 胡睿铭, 吴斌. 华中地区猪繁殖与呼吸综合征病毒临床流行毒株Nsp2和GP5氨基酸的遗传变异分析[J]. , 2015, 42(11): 3026-3036.

LI Bin, WANG Hao-nan, PENG Zhong, HU Rui-ming, WU Bin. Genetic Variation Analysis of Nsp2 and GP5 Amino Acids of Porcine Reproductive and Respiratory Syndrome Virus in Clinical in Central China[J]. , 2015, 42(11): 3026-3036.

参考文献

[1] 斯特劳,阿莱尔,蒙加林等主编.赵德明,张仲秋,沈建忠等译.猪病学[M].第8版.北京:中国农业大学出版社,2000.
[2] Albina E.Reproductive and respiratory syndrome:Ten years of experience (1986-1996) with this undesirable viral infection[J].Vet Res,1997,28(4):305-320.
[3] Wensvoort G.Lelystadvirus and the porcine epidemic abortion and respiratory syndrome[J].Vet Res,1993,24(2):117-124.
[4] 郭宝清,陈章水,刘文兴,等.从疑似PPRS流产胎儿分离PRRSV的研究[J].中国畜禽传染病,1996,18(2):1-4.
[5] Barrette R W,Metwally S A,Rowland J M,et al.Discovery of swine as a host for the restonebolavirus[J].Science,2009,325(5937):204-206.
[6] Dea S,Gagnon C A,Mardassi H,et al.Current knowledge on the structural proteins of porcine reproductive and respiratory syndrome virus:Comparison of the North American and European isolates[J].Archives of Virology,2000,145(4):659-688.
[7] Otake S,Dee S A,Moon R D,et al.Evaluation of mosquitoes,aedesvexans,as biological vectors of porcine reproductive and respiratory syndrome virus[J].Can J Vet Res,2003,67(4):265-270.
[8] Chen N,Cao Z,Yu X,et al.Emergence of novel European genotype porcine reproductive and respiratory syndrome virus in mainland China[J].Journal of General Virology,2011,92(4):880-892.
[9] Mu C,Lu X,Duan E,et al.Molecular evolution of porcine reproductive and respiratory syndrome virus isolates from central China[J].Research in Veterinary Science,2013,95(3):908-912.
[10] Li B,Fang L,Guo X,et al.Epidemiology and evolutionary characteristics of the porcine reproductive and respiratory syndrome virus in China between 2006 and 2010[J].Journal of Clinical Microbiology,2011,49(9):3175-3183.
[11] Han J,Wang Y,Faaberg K S.Complete genome analysis of RFLP 184 isolates of porcine reproductive and respiratory syndrome virus[J].Virus Research,2006,122(1):175-182.
[12] Zhou Y J,Hao X F,Tian Z J,et al.Highly virulent porcine reproductive and respiratory syndrome virus emerged in China[J].Transboundary and Emerging Diseases,2008,55(3-4):152-164.
[13] Leng C,Tian Z,Zhang W,et al.Characterization of two newly emerged isolates of porcine reproductive and respiratory syndrome virus from Northeast China in 2013[J].Veterinary Microbiology,2014,171(1):41-52.
[14] Zhou L,Zhang J,Zeng J,et al.The 30-amino-acid deletion in the Nsp2 of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China is not related to its virulence[J].Journal of Virology,2009,83(10):5156-5167.
[15] Mengeling W L,Lager K M,Vorwald A C.Diagnosis of porcine reproductive and respiratory syndrome[J].Journal of Veterinary Diagnostic Investigation,1995,7(1):3-16.
[16] Ostrowski M,Galeota J A,Jar A M,et al.Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain[J].Journal of Virology,2002,76(9):4241-4250.
[17] 唐娜,管宇,魏凤,等.2006-2012年鲁豫冀地区15株猪繁殖与呼吸综合征病毒的分离鉴定及ORF5/Nsp2基因的遗传变异分析[J].中国畜牧兽医,2013,40(10):26-32.
[18] Mateu E,Diaz I.The challenge of PRRS immunology[J].The Veterinary Journal,2008,177(3):345-351.
[19] 杨汉春.主要猪病流行现状、防控面临的问题与对策[J].兽医导刊,2014,9:38-40.
[20] Darwich L,Díaz I,Mateu E.Certainties,doubts and hypotheses in porcine reproductive and respiratory syndrome virus immunobiology[J]. Virus Research,2010,154(1):123-132.

华中地区猪繁殖与呼吸综合征病毒临床流行毒株Nsp2和GP5氨基酸的遗传变异分析

Genetic Variation Analysis of Nsp2 and GP5 Amino Acids of Porcine Reproductive and Respiratory Syndrome Virus in Clinical in Central China

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	卢玉葵, 刘佳琪, 钟嘉诚, 陈济铛, 朱婉君, 张溢珊, 张济培. 鹅源副鸡禽杆菌的分离鉴定及致病性研究[J]. 中国畜牧兽医, 2023, 50(6): 2562-2572.
[2]	康溥, 任照文, 蔡汝健, 吕宗吉, 黄育浩, 王晓虎. 广东及周边省份猪繁殖与呼吸综合征病毒2型谱系8毒株变异规律和传播研究[J]. 中国畜牧兽医, 2023, 50(5): 2001-2011.
[3]	陈天宝, 王娅妮, 刘宝玲, 王刚, 王晓虎, 柳旭辉, 蔡汝健, 贺东生. 中药抗猪繁殖与呼吸综合征病毒的作用机制研究进展[J]. 中国畜牧兽医, 2023, 50(5): 2114-2129.
[4]	李志杰, 赵莹, 马鑫, 王萌, 余四九, 王立斌, 潘阳阳. 动物卵泡液外泌体分离方法及主要生殖功能研究进展[J]. 中国畜牧兽医, 2023, 50(4): 1480-1488.
[5]	田常青, 张坤中, 齐玉梅, 史文静, 董志杰, 张浩浩, 何曾文, 芝吉, 赵学慧, 崇倩, 薛慧文, 苟惠天. 1株环境源单增李斯特菌的分离鉴定及生物特性分析[J]. 中国畜牧兽医, 2023, 50(4): 1543-1555.
[6]	曹颖颖, 梁亮, 曾怡蓉, 钟清华, 袁小芳, 唐海波, 冷静. 滇西树鼩源沙门氏菌的分离鉴定及生物学特性研究[J]. 中国畜牧兽医, 2023, 50(4): 1718-1728.
[7]	李华玮, 郭科威, 王旭英, 张小雨, 路紫微, 李新锋, 马辉, 侯文静. 猪繁殖与呼吸综合征病毒非结构蛋白7真核表达及亚细胞定位[J]. 中国畜牧兽医, 2023, 50(3): 1160-1168.
[8]	王冰艺, 马弘财, 邹明昊, 樊世杰, 元振杰, 拉巴次仁, 董海龙, 曾江勇. 牦牛源链球菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医, 2023, 50(2): 745-753.
[9]	肖洋洋, 李芮芮, 马忠臣, 唐恬, 王娜, 陈创夫, 郑炜, 王勇, 王鹏雁. 新疆石河子地区牛支原体的分离鉴定及药物敏感性分析[J]. 中国畜牧兽医, 2023, 50(2): 754-763.
[10]	钟华晨, 王丽芳, 郭晨阳, 刘嘉琳, 宋洁. 内蒙古地区乳房炎奶样与环境中细菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医, 2023, 50(2): 817-826.
[11]	孙愉, 张昊, 李畅洋, 陶燕子, 王秋菊. 牛源抗氧化芽孢杆菌的分离鉴定及生物特性研究[J]. 中国畜牧兽医, 2023, 50(1): 359-367.
[12]	魏津, 王甲, 赵浩然, 刘博, 刘元杰, 姚文生, 刘燕, 马欣. 不同地区鸡毒支原体的分离鉴定及药物敏感性研究[J]. 中国畜牧兽医, 2023, 50(1): 368-376.
[13]	杨福梅, 杨林富, 曾成, 杨志, 王康, 段纲, 代飞燕. 1株麂子源麦芽香肉杆菌的分离鉴定及生物学特性分析[J]. 中国畜牧兽医, 2023, 50(1): 398-407.
[14]	张薇, 武华, 阴雅洁, 李松励, 侯绍华. 新型鸭呼肠孤病毒分离鉴定及其σC基因序列分析[J]. 中国畜牧兽医, 2022, 49(9): 3520-3529.
[15]	席静, 易继海, 王月丽, 徐朕宇, 李培东, 张江伟, 陈创夫. 牛A群轮状病毒G10型XJ-2022株分离鉴定及基因型分析[J]. 中国畜牧兽医, 2022, 49(9): 3530-3538.