RNA-seq技术在寄生虫研究中的应用

doi:10.16431/j.cnki.1671-7236.2015.11.014

摘要/Abstract

摘要： 转录组测序(RNA-seq)是通过高通量测序方法全面快速的获悉特定组织或细胞的特定发育阶段或功能状态下所有RNA序列信息的技术。该技术以其准确和高通量等特征,被广泛应用于生物学、医学和农学等基础性研究。寄生虫在体外发育、感染入侵和体内繁殖等不同阶段,发生着一系列复杂的生物变化。采用RNA-seq技术筛选寄生虫发育、感染和繁殖等不同阶段的差异表达基因,阐明其功能,获悉寄生虫-宿主互作的分子调控机制等研究是目前的研究重点和热点。基于此,作者对RNA-seq技术在寄生虫研究中的应用作一综述。

关键词: 转录组测序; 差异表达基因; 寄生虫

Abstract: Transcriptome sequencing technology (RNA-seq) is to inform all RNA sequences on a specific developmental stage or functional state in a particular tissue or cell through the high throughput sequencing method.The technology is widely used in biology,medicine,agriculture and other basic research,mainly because of accurate and high-throughput,as a powerful research tool.During the different stages of parasites,including development,infection and propagation,a series of complex biological changes occur.The differential expression genes in different stages of parasite development,function about the genes and the interactions in host-parasite are the key focus of current research.On the basis of the above,the summary of the application of RNA-seq technology in parasite research is summarized.

Key words: transcriptome sequencing; differential expression gene; parasite

中图分类号:

于馨, 杨艳, 胡双, 杨建发, 邹丰才. RNA-seq技术在寄生虫研究中的应用[J]. , 2015, 42(11): 2909-2914.

YU Xin, YANG Yan, HU Shuang, YANG Jian-fa, ZOU Feng-cai. Application of RNA-seq Technology in Parasites Research[J]. , 2015, 42(11): 2909-2914.

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