延边黄牛体细胞核移植的研究进展

doi:10.16431/j.cnki.1671-7236.2015.10.034

摘要/Abstract

摘要： 牛体细胞核移植(somatic cell nuclear transfer,SCNT)是一套极其复杂的技术体系,它包括卵母细胞的成熟、供核细胞的准备、卵母细胞的显微操作、细胞融合、卵母细胞激活和胚胎培养。因此,许多因素影响着核移植胚胎发育。虽然陆续有克隆牛出生的报道,但是克隆效率依然低下。本综述对延边黄牛体细胞核移植体系的6个方面进行简单综述,以期为探究延边黄牛体细胞克隆的最优化条件、建立最优化的培养体系、提高克隆效率提供参考。

关键词: 延边黄牛; 哺乳动物; 体细胞克隆; 核移植

Abstract: Bovine somatic cell nuclear transfer (SCNT) is a sophisticated technique system,including oocyte maturation,donor cell preparation and oocytes microscopic operation,fusion,activation and culture.Although the birth of cloning cattle has been reported recently,the efficiency of somatic cell cloning has remained lowly.In order to establish the optimization somatic cell nuclear transfer system of Yanbian Yellow cattle,this review summarized only from 6 main aspects mentioned above in this field.

Key words: Yanbian Yellow cattle; mammals; somatic cell clone; nuclear transfer

中图分类号:

S823.8⁺1

徐礼杰, 孙武胜, 胡德宝, 方南洙, 李钟淑, 李福俊. 延边黄牛体细胞核移植的研究进展[J]. , 2015, 42(10): 2739-2744.

XU Li-jie, SUN Wu-sheng, HU De-bao, FANG Nan-zhu, LI Zhong-shu, Li Fu-jun. Research Advances on Somatic Cell Nuclear Transfer in Yanbian Yellow Cattle[J]. , 2015, 42(10): 2739-2744.

参考文献

[1] Thuan N V,Kishigami S,Wakayama T.How to improve the success rate of mouse cloning technology[J].J Reprod Dev,2010,56(1):20-30.
[2] Wakayama T,Yanagimachi R.Cloning of male mice from adult tail-tip cells[J].Nat Genet,1999,22(2):127-128.
[3] Tanaka S,Oda M,Toyoshima Y,et al.Placentomegaly in cloned mouse concepti caused by expansion of the spongiotrophoblast layer[J].Biol Reprod,2001,65(6):1813-1821.
[4] Tamashiro K L,Wakayama T,Akutsu H,et al.Cloned mice have an obese phenotype not transmitted to their offspring[J].Nat Med,2002,8(3):262-267.
[5] Tamashiro K L,Wakayama T,Blanchard R J,et al.Postnatal growth and behavioral development of mice cloned from adult cumulus cells[J].Biol Reprod,2000,63(1):328-334.
[6] Ogonuki N,Inoue K,Yamamoto Y,et al.Early death of mice cloned from somatic cells[J].Nat Genet,2002,30(3):253-254.
[7] Rodriguez-Osorio N,Urrego R,Cibelli J B,et al.Reprogramming mammalian somatic cells[J].Theriogenology,2012,78(9):1869-1886.
[8] 施玉海,孙玉海,王君,等.浅析延边黄牛人工授精技术应用现状及对策[J].吉林畜牧兽医,2011,2(32):33-34.
[9] Sirard M A,Richard F,Blondin P,et al.Contribution of the oocyte to embryo quality[J]. Theriogenology,2006,65(1):126-136.
[10] Wongsrikeao P,Nagai T,Agung B,et al.Improvement of transgenic cloning efficiencies by culturing recipient oocytes and donor cells with antioxidant vitamins in cattle[J].Molecular Reproduction and Development,2007,74(6):694-702.
[11] Naruse K,Iga K,Shimizu M,et al.Milrinone treatment of bovine oocytes during in vitro maturation benefits production of nuclear transfer embryos by improving enucleation rate and developmental competence[J].Journal of Reproduction and Development,2012,58(4):476-483.
[12] 韩岩,庄利利,王玉涵,等.谷氨酰胺对延边黄牛卵母细胞成熟及重组胚发育的影响[J].畜牧与兽医,2010,42(5):11-14.
[13] 胡艳明,祖晶,孙焕林,等.FSH和LH对延边黄牛卵母细胞体外成熟及重组胚发育的影响[J].繁殖生理,2009,45(11):18-22.
[14] Rocha A,Randel R D,Broussard J R,et al.High environmental temperature and humidity decrease oocyte quality in Bos taurus but not in Bos indicus cows[J].Theriogenology,1998,49(3):657-665.
[15] 苗婷婷.不同成熟因素对延边黄牛卵母细胞体外成熟及核移植后胚胎早期发育[D].延吉:延边大学,2007.
[16] Prokofiev M I,Ernst L K,Suraeva N M,et al.Bovine oocyte maturation,fertilization and further development in vitro and after transfer into recipients[J].Theriogenology,1992,38(3):461-469.
[17] Kato Y,Tani T,Tsunoda Y.Cloning of calves from various somatic cell types of male and female adult,newborn and fetal cows[J].Journal of Reproduction and Fertility,2000,120(2):231-237.
[18] Kishi M,Itagaki Y,Takakura R,et al.Nuclear transfer in cattle using colostrum-derived mammary gland epithelial cells and ear-derived fibroblast cells[J].Theriogenology,2000,54(5):675-684.
[19] Kato Y,Tani T,Sotomaru Y,et al.Eight calves cloned from somatic cells of a single adult[J].Science,1998,282(5396):2095-2098.
[20] Goto Y,Kaneyama K,Kobayashi S,et al.Birth of cloned calves derived from cultured oviductal epithelial cells of a dairy cow[J].Animal Science Journal,1999,70(4):243-245.
[21] Tian X C,Kubota C,Enright B,et al.Cloning animals by somatic cell nuclear transfer-biological factors[J].Reproductive Biology and Endocrinology,2003,1(1):98-104.
[22] Campbell K H,Alberio R,Choi I,et al.Cloning:Eight years after Dolly[J].Reproduction in Domestic Animals,2005,40(4):256-268.
[23] Kato Y,Tsunoda Y.Role of the donor nuclei in cloning efficiency:Can the ooplasm reprogram any nucleus[J].The International Journal of Developmental Biology,2010,54(11-12):1623-1629.
[24] Kubota C,Yamakuchi H,Todoroki J,et a1.Six cloned calves produced from adult fibroblast cells after long-term culture[J].Proc Natl Acad Sci,2000,97(3):990-995.
[25] 黄玲.延边黄牛成纤维细胞培养、冷冻保存及核移植效率的研究[D].延吉:延边大学,2007.
[26] Cibelli J B,Stice S L,Golueke P J,et al.Cloned transgenic calves produced from nonquiescent fetal fibroblasts[J].Science,1998,280(5367):1256-1258.
[27] Wells D N,Misica P M,Tervit H R.Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells[J].Biol Reprod,1999,60(4):996-1005.
[28] Lanza R P,Cibelli J B,Blackwell C,et al.Extension of cell life-span and telomere length in animals cloned from senescent somatic cells[J].Science,2000,288(5466):665-669.
[29] 郭杰.冷冻保存对延边黄牛体细胞培养及核移植效率的影响[D].延吉:延边大学,2008.
[30] McGrath J,Solter D.Nuclear transplantation in the mouse embryo by microsurgery and cell fusion[J].Science,1983,220(4603):1300-1302.
[31] Heyman Y,Chesné P,Lebourhis D,et al.Developmental ability of bovine embryos after nuclear transfer based on the nuclear source:In vivo versus in vitro[J].Theriogenology,1994,42(4):695-702.
[32] Smith L C,Wilmut I.Factors affecting the viability of nuclear transplanted-embryos[J].Theriogenology,1990,1(33):153-164.
[33] Yin X J,Tani T,Yonemura I,et al.Production of cloned pigs from adult somatic cells by chemically assisted removal of maternal chromosomes[J].Biol Reprod,2002,67(2):442-446.
[34] Smith L C.Membrane and intracellular effects of ultraviolet irradiation with Hoechst 33342 on bovine secondary oocytes matured in vitro[J].J Reprod Fertil,1993,99(1):39-44.
[35] Tatham B G,Dowsing A T,Trounson A O.Enucleation by centrifugation of in vitro-matured bovine oocytes for use in nuclear transfer[J].Biology of Reproduction,1995,53(5):1088-1094.
[36] 李井春.延边黄牛体细胞克隆卵母细胞显微操作方法的研究[D].延吉:延边大学,2007.
[37] 李井春,方南沫.延边黄牛卵母细胞去核方法的研究[J].中国牛业科学,2006,32(5):19-22.
[38] 王艳多.化学诱导去核法在延边黄牛体细胞核移植中的应用研究[D].延吉:延边大学,2008.
[39] Bordignon V,Smith L C.Telophase enucleation:An improved method to prepare recipient cytoplasts for use in bovine nuclear transfer[J].Mol Reprod Dev,1998,49(1):29-36.
[40] 林涛.末期去核在延边黄牛体细胞核移植中的应用研究[D].延吉:延边大学,2008.
[41] Tani T,Shimada H,Kato Y,et al.Demecolcine-assisted enucleation for bovine cloning[J].Cloning Stem Cells,2006,8(1):61-66.
[42] 王玉涵.化学辅助去核法在延边黄牛体细胞核移植中应用的研究[D].延吉:延边大学系,2011.
[43] Karja N W,Otoi T,Wongsrikeao P,et al.Effects of electric field strengths on fusion and in vitro development of domestic cat embryos derived by somatic cell nuclear transfer[J].Theriogenology,2006,66(5):1237-42.
[44] 杨月春,李钟淑,王士勇,等.延边黄牛体细胞克隆融合条件的研究[J].延边大学农学学报,2009,31(3):153-158.
[45] 李晓霞.延边黄牛体细胞克隆电融合的研究[D].延吉:延边大学,2008.
[46] 宋君博,林涛,胡艳明,等.细胞松弛素B对延边黄牛体细胞核移植效率的影响[J].河南农业科学,2009,4:123-126.
[47] 宋君博.细胞松弛素B和咖啡因对延边黄牛体细胞克隆效率的影响[D].延吉:延边大学,2009.
[48] Kishigami S,Mizutani E,Ohta H,et al.Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer[J].Biochem Biophys Res Commun,2006,340(1):183-189.
[49] Svensson K,Mattsson R,James T C,et al.The paternal allele of the H19 gene is progressively silenced during early mouse development:The acetylation status of histones may be involved in the generation of variegated expression patterns[J].Development,1998,125(1):61-69.
[50] 高璐.组蛋白去乙酰化酶抑制剂(TSA)对延边黄牛体细胞克隆效率的影响[D].延吉:延边大学,2011.
[51] 王士勇,李钟淑,金庆国,等.共培养对延边黄牛重组胚体外发育的影响[J].延边大学农学学报,2008,30(3):192-196.
[52] Nasresfahani M H,Aitken R J,Johnson M H.The effect of iron and iron chelators on the in-vitro block to development of the mouse preimplantation embryo:BAT6 a new medium for improved culture of mouse embryos in vitro[J].Hum Reprod,1990,5(8):997-1003.
[53] Nukayama T,Neda Y,Goto Y.Effects of visible light and other environmental factors on the production of oxygen radicals by hamster embryos[J].Theriogenology,1994,41(2):499-510.
[54] 杨月春,王士勇,李钟淑,等.延边黄牛核移植胚胎的活性氧含量的测定[J].西南农业学报,2010,23(1):210-213.
[55] 李丽.巯基物质对牛卵母细胞体外成熟及体细胞克隆胚胎体外发育的影响[D].延吉:延边大学,2010.
[56] Oback B,Wells D N.Cloning cattle:The methods in the madness[J].Adv Exp Med Biol,2007,591:30-57.
[57] Ross P J,Cibelli J B.Bovine somatic cell nuclear transfer[J].Methods Mol Biol,2010,636:155-177.
[58] Tian X C,Kubota C,Sakashita K,et al.Meat and milk compositions of bovine clones[J].Proc Natl Acad Sci USA,2005,102(18):6261-6266.
[59] Hoshino Y,Hayashi N,Taniguchi S,et al.Resurrection of a bull by cloning from organs frozen without cryoprotectant in a-80 degrees c freezer for a decade[J].PLoS One,2009,4(1):e4142.
[60] Yonai M,Kaneyama K,Miyashita N,et al.Growth,reproduction,and lactation in somatic cell cloned cows with short telomeres[J].J Dairy Sci,2005,88(11):4097-4110.
[61] Wells D N,Misica P M,Tervit H R,et al.Adult somatic cell nuclear transfer is used to preserve the last surviving cow of the Enderby Island cattle breed[J].Reprod Fertil Dev,1998,10(4):369-378.
[62] Richt J A,Kasinathan P,Hamir A N,et al.Production of cattle lacking prion protein[J]. Nat Biotechnol,2007,25(1):132-138.
[63] Brophy B,Smolenski G,Wheeler T,et al.Cloned transgenic cattle produce milk with higher levels of beta-casein and kappa-casein[J].Nat Biotechnol,2003,21(2):157-162.
[64] Panarace M,Agüero J I,Garrote M,et al.How healthy are clones and their progeny:5 years of field experience[J].Theriogenology,2007,67(1):142-151.
[65] Watanabe S,Nagai T.Survival of embryos and calves derived from somatic cell nuclear transfer in cattle:A nationwide survey in Japan[J].Animal Science Journal,2011,82(2):360-365.
[66] Akagi S,Geshi M,Nagai T.Recent progress in bovine somatic cell nuclear transfer[J].Anim Sci J,2013,84(3):191-199.
[67] Sugimura S,Akai T,Hashiyada Y,et al.Promising system for selecting healthy in vitro-fertilized embryos in cattle[J].PLoS One,2012,7(5):e36627.