基于RNA-Seq数据分析环格列酮和胰岛素诱导延边黄牛前脂肪细胞分化的差异表达基因

doi:10.16431/j.cnki.1671-7236.2021.07.010

摘要/Abstract

摘要： 试验旨在探讨过氧化物酶体增殖物激活受体γ（peroxisome proliferators-activated receptors，PPARγ）激动因子（环格列酮）和胰岛素对延边黄牛前脂肪细胞脂质代谢的影响及可能的调控机制。试验分为对照组（CON，5% FBS）、胰岛素处理组（I组，5% FBS+10 mg/L胰岛素）、环格列酮处理组（C组，5% FBS+10 mg/L环格列酮）、胰岛素+环格列酮处理组（IC组，5% FBS+10 mg/L胰岛素+10 mg/L环格列酮）。各组处理144 h，通过RNA-Seq技术对其进行转录本测序，差异表达基因进行GO功能注释和KEGG富集分析。差异表达基因分析结果显示，与对照组相比，IC组差异表达基因数量为1 764个，比I和C组分别多276和569个，3个试验组间共有371个差异基因；IC组上调基因数量为974个，分别比I和C组多140和249个，IC组下调基因为790个，I和C组下调基因分别为654和470个。差异基因的GO分析表明，各处理组的差异基因参与了细胞过程、代谢过程以及生物过程的调节；在分子功能上，主要是分子功能调节剂、转运活性、转录调节活性等；在细胞成分上，大多数差异基因被富集到细胞器、细胞膜及胞外区等。KEGG通路分析发现，差异表达基因主要富集在FoxO、PPAR、TGF-β、p53等信号通路。综上所述，胰岛素和环格列酮单独处理与二者共同处理对延边黄牛前脂肪细胞分化的调控机制有所差别，其中二者共同处理对分化的促进作用更加明显。本研究结果为研究环格列酮与胰岛素在调节脂肪生成中的功能和分子机制提供了依据。

关键词: RNA-Seq; 胰岛素; 环格列酮; 延边黄牛; 前脂肪细胞

Abstract: The purpose of this study was to investigate the effects of peroxisome proliferators activated receptors (PPARγ) agonist (ciglitazone) and insulin on lipid metabolism and its regulatory mechanism in preadipocytes of Yanbian Yellow cattle.The experiments were divided into control group (CON,5% FBS),insulin treatment group (I,5% FBS+10 mg/L insulin),cycloglitazone treatment group (C,5% FBS+10 mg/L insulin+10 mg/L cycloglitazone) and insulin+cycloglitazone treatment group (IC,5% FBS+10 mg/L insulin+10 mg/L cycloglitazone).Each group was treated for 144 h,and transcriptome sequencing was performed on the preadipocytes by RNA-Seq technology,and the differentially expressed genes were analyzed by GO functional annotation and KEGG enrichment.Analysis results of differentially expressed genes(DEGs) in each treatment group showed that:Compared with control group,the number of DEGs in IC group was 1 764,276 and 569 more than that in I and C groups,respectively.There were 371 DEGs in each treatment group.The number of up-regulated genes in IC group was 974,140 and 249 more than that in I and C groups,respectively,while the number of down-regulated genes in IC group was 790,I and C groups was 654 and 470,respectively.The GO analysis of the DEGs showed that the DEGs in each treatment group were involved in the regulation of cellular processes,metabolic processes and biological processes.In terms of molecular function,it mainly included molecular function regulator,transport activity and transcriptional regulation activity.In terms of cell composition,most of the DEGs were enriched into organelles,membranes and extracellular regions.KEGG pathway analysis showed that the DEGs were mainly concentrated in FoxO signaling pathway,PPAR signaling pathway,TGF signaling pathway,p53 signaling pathway,etc.In conclusion,the regulation mechanism of preadipocyte differentiation of Yanbian Yellow cattle treated with insulin and cycloglazidone alone was different from that treated with cycloglazidone together,and the promotion effect of the two treatments on differentiation was more obvious.The results of this study provided a basis for studying the function and molecular mechanism of cycloglitazone and insulin in regulating fat production.

Key words: RNA-Seq; insulin; ciglirtazone; Yanbian Yellow cattle; preadipocytes

中图分类号:

S823

郭盼盼, 金鑫, 孙建富, 李香子, 尹云厚, 严昌国. 基于RNA-Seq数据分析环格列酮和胰岛素诱导延边黄牛前脂肪细胞分化的差异表达基因[J]. 中国畜牧兽医, 2021, 48(7): 2358-2368.

GUO Panpan, JIN Xin, SUN Jianfu, LI Xiangzi, YIN Yunhou, YAN Changguo. Analysis of Differentially Expressed Genes in Differentiation of Yanbian Yellow Cattle Preadipocytes Induced by Cycloglitazone and Insulin Based on RNA-Seq Data[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2358-2368.

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