《中国畜牧兽医》---唯一指定的官方网站 ›› 2015, Vol. 42 ›› Issue (9): 2453-2458.doi: 10.16431/j.cnki.1671-7236.2015.09.035

• 遗传繁育 • 上一篇    下一篇

伊犁马催乳素受体基因的遗传多态性及其与产奶量的关联分析

于茜, 臧长江, 刘玲玲, 马海玉, 何美升, 姜鑫, 曹行, 刘武军   

  1. 新疆农业大学动物科学学院, 乌鲁木齐 830052
  • 修回日期:2015-07-20 出版日期:2015-09-20 发布日期:2015-09-25
  • 通讯作者: 刘武军 E-mail:lwj_ws@163.com
  • 作者简介:于茜(1991-),女,新疆人,硕士生,研究方向:动物遗传育种,E-mail:331379406@qq.com
  • 基金资助:

    国家科技支撑项目(2012BAD44B01);新疆维吾尔自治区重大专项(201130101-3)

The Association Analysis of Prolactin Receptor Gene Polymorphisms with Milk Yield in Yili Horse

YU Xi, ZANG Chang-jiang, LIU Ling-ling, MA Hai-yu, HE Mei-sheng, JIANG Xin, CAO Hang, LIU Wu-jun   

  1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
  • Revised:2015-07-20 Online:2015-09-20 Published:2015-09-25

摘要: 试验旨在研究伊犁马催乳素受体(prolactin receptor,PRLR)基因多态性及其与产奶量之间的关联性。以60匹伊犁马为研究对象,选取PRLR基因侧翼区的部分片段,采用PCR-SSCP技术检测其遗传多态性,并与伊犁马的日产奶量进行关联分析,研究PRLR基因的多态性与伊犁马产奶性能的关系。结果显示,PRLR基因侧翼区的2个片段均具有多态性,都存在3种基因型:AA、AB和BB,测序结果显示PRLR-P1的碱基突变位置为g.29764513 G>A,该突变为错义突变,导致其编码的氨基酸由组氨酸变成精氨酸,经关联分析发现,该突变对伊犁马日产奶量有显著影响(P<0.05);PRLR-P2的碱基突变位置为g.30106804 C>A,该突变为同义突变,未导致其编码的氨基酸发生改变,经关联分析发现,该突变对伊犁马日产奶量有显著影响(P<0.05)。伊犁马PRLR基因侧翼区的碱基突变与日产奶量之间存在关联性,且可能是影响伊犁马产奶性能的重要位点。

关键词: 伊犁马; PRLR基因; 产奶量

Abstract: This experiment was designed to analyze association of prolactin receptor (PRLR) gene polymorphism with milk yield of Yili horse.The number of 60 horse were selected as samples, detected genetic polymorphism of PRLR gene using PCR-SSCP technique and sequencing technology, and then analyzed the polymorphism with milk yield of Yili horse.The results showed that there were two polymorphism fragments of PRLR gene flanking region, there were three genotypes:AA, AB and BB.Two novel SNPs (g.29764513 G>A and g.30106804 C>A) were identified of PRLR gene flanking region by sequencing.The g.29764513 G>A SNP caused amino acid variations as p.33His>Arg, while the g.30106804 C>A SNP was synonymous mutation without causing amino acid changes.Statistical results indicated that the g.29764513 G>A and g.30106804 C>A SNPs were significantly associated with daily milk yield in Yili horse (P<0.05).These mutations might be as a crucial DNA genetic markersfor milk production traits selection in Yili horse.

Key words: Yili horse; PRLR gene; milk yield

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