氮磷营养盐条件下大肠杆菌对氯霉素耐药性及机理研究

doi:10.16431/j.cnki.1671-7236.2015.06.038

摘要/Abstract

摘要： 为探讨氮磷营养盐对环境大肠杆菌(E.coli)耐药性的影响及其机制,本试验通过建立模型生态系统研究氮磷营养盐引发大肠杆菌对氯霉素(CHL)产生耐药表型的影响,并对分离到的耐药菌株和敏感菌株进行cat基因检测.结果显示,添加不同剂量氮磷营养盐可引发大肠杆菌对氯霉素产生耐药性,46株耐氯霉素大肠杆菌cat基因的阳性率为89.13%;16株对氯霉素敏感的大肠杆菌未检出cat基因.结果表明,模拟生态系统中加入氮磷营养盐可引发大肠杆菌对氯霉素产生耐药;氮磷营养盐引发大肠杆菌对氯霉素耐药与cat基因有关.

关键词: 大肠杆菌; 模型生态系统; 氮磷营养盐; 耐药; cat基因

Abstract: This study was designed to explore the effect of nitrogen and phosphorous on the resistance of E.coli from environment and the mechanism.Microcosms were established to study the effect of nitrogen and phosphorous on the resistant phenotype of E.coli to chloramphenicol (CHL).cat gene of isolated drug-resistant strains and susceptible strains were detected.The results showed that,different concentration of nitrogen and phosphorous could induce the formation of antibiotics resistance of E.coli to CHL.The rate of cat gene of 46 strains of chloramphenicol resistant E.coli was 89.13%,which was 0 in the 16 strains of chloramphenicol sensitive E.coli.The results indicated that,nitrogen and phosphorous in the microcosms could induce the formation and maintenance of resistance to chloramphenicol in E.coli,which had correlation with cat gene.

Key words: E.coli; microcosm; nitrogen and phosphorus; resistance; cat gene

中图分类号:

S852.61

黄启发, 陈秀萍, 林晶, 张洁, 汪燕秋, 俞道进. 氮磷营养盐条件下大肠杆菌对氯霉素耐药性及机理研究[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(6): 1580-1586.

HUANG Qi-fa, CHEN Xiu-ping, LIN Jing, ZHANG Jie, WANG Yan-qiu, YU Dao-jin. Study on Effect of Nitrogen and Phosphorous on the Resistance of E.coli to Chloramphenicol and its Mechanism[J]. , 2015, 42(6): 1580-1586.

参考文献

[1] 田永杰,唐志坚,李世斌.我国湖泊富营养化的现状和治理对策[J].环境科学与管理,2006,31(5):119-121.

[2] 秦伯强,许海,董百丽.富营养化湖泊治理的理论与实践[M].北京:高等教育出版社,2011.

[3] Lehtola M J,Miettinen I T,Martikainen P J.Biofilm formation in drinking water affected by low concentrations of phosphorus[J].Canadian Journal of Microbiology,2002,48(6):494-499.

[4] Sathasivan A,Ohgaki S.Application of new bacterial regrowth potential method for water distribution system——A clear evidence of phosphorus limitation[J].Water Research,1999,33(1):137-144.

[5] Keinnen M M,Korhonen L K,Lehtola M J,et al.The microbial community structure of drinking water biofilms can be affected by phosphorus availability[J].Applied and Environmental Microbiology,2002,68(1):434-439.

[6] Podbielski A,Kreikemeyer B.Cell density——Dependent regulation:Basic principles and effects on the virulence of gram-positive cocci[J].International Journal of Infectious Diseases,2004,8(2):81-95.

[7] Tan S L,Lee H Y,Mahyudin N A.Antimicrobial resistance of Escherichia coli and Staphylococcus aureus isolated from food handler's hands[J].Food Control,2014,44:203-207.

[8] Nielsen I W,Bakke I,Vader A,et al.Isolation of cmr,a novel Escherichia coli chloramphenicol resistance gene encoding a putative efflux pump[J].Bacteriol,1996,178(11):3188-3193.

[9] Hamelin K,Bruant G,El-Shaarawi A,et al.Occurrence of virulence and antimicrobial resistance genes in Escherichia coli isolates from different aquatic ecosystems within the St.Clair River and Detroit River areas [J].Applied and Environmental Microbiology,2007,73(2):477-484.

[10] Anderson M,Sobsey M,Rose J,et al.Detection and occurrence of antimicrobially resistant Escherichia coli in ground-water on or near swine farms in Eastern North Carolina[J].Water Science and Technology,2006,54:211-218.

[11] 赵明秋,沈海燕,潘文,等.细菌耐药性产生的原因、机制及防治措施[J].中国畜牧兽医,2011,38(5):177-181.

[12] Zheng S,Qiu X,Chen B,et al.Antibiotics pollution in Jiulong River estuary:Source,distribution and bacterial resistance[J].Chemosphere,2011,84(11):1677-1685.

[13] Ran T.Detection of antibiotic resistance and tetracycline resistance genes in Enterobacteriaceae isolated from the Pearl Rivers in South China[J].Environ Pollut,2010,158(6):2101-2109.

[14] Su H C,Ying G G,Tao R,et al.Class 1 and 2 integrons,sul resistance genes and antibiotic resistance in Escherichia coli isolated from Dongjiang River,South China[J].Environ Pollut,2012,169:42-49.

[15] 高璐琰,杨钟,武瑞兵,等.细菌耐药性研究进展[J].中国畜牧兽医,2013,40(7):162-166.

[16] 郑丙辉,许秋瑾,朱延忠.湖泊营养盐控制标准制订方法的初步研究[J].环境科学,2009,30(9):2497-2501.

[17] 宋玉芝,杨美玖,秦伯强.苦草对富营养化水体中氮磷营养盐的生理响应[J].环境科学,2011,32(9):2570-2575.

[18] 雷衍之.养殖水环境化学实验[M].北京:中国农业出版社,2007.

[19] 邢殿楼,霍堂斌,吴会民,等.总磷、总氮联合消化的测定方法[J].大连水产学院学报,2006,21(3):220-225.

[20] Karin S,Christina H,Johann B.Resistance gene patterns of tetracycline resistant Escherichia coli of human and porcine origin[J].Veterinary Microbiology,2010,142(3):329-336.

[21] Yolanda S,Laura B,Elena D,et al.Mechanisms of resistance in multiple-antibiotic-resistant Escherichia coli strains of human,animal,and food origins[J].Antimicrobial Agents and Chemotherapy,2004,48(10):3996-4000.

[22] Yu D J,Yi X L,Ma Y F,et al.Effect of administration mode of antibiotics on antibiotic resistance of Enterococcus faecalis in aquatic ecosystem[J].Chemosphere,2009,76(7):915-920.

[23] Yu D J,Lai B S,Ma Y F,et al.Cornmeal-induced resistance to ciprofloxacin and erythromycin in enterococci[J].Chemosphere,2012,89:70-75.

[24] McPhearson R M,DePaola A,Zywno S R,et al.Antibiotic resistance in gram-negative bacteria from cultured catfish and aquaculture ponds[J].Aquaculture,1991,99(3):203-211.

[25] Kapetanaki M,Kerry J,Hiney M,et al.Emergence,in oxytetracycline-free marine mesocosms,of microorganisms capable of colony formation on oxytetracycline-containing media[J].Aquaculture,1995,134(3):227-236.

[26] Florian T,Adatte T,Wildi W,et al.Antibiotic resistant bacteria/genes dissemination in lacustrine sediments highly increased following cultural eutrophication of Lake Geneva (Switzerland)[J].Chemosphere,2012,86(5):468-476.

[27] Johnson P T J,Townsend A R,Cleveland C C,et al.Linking environmental nutrient enrichment and disease emergence in humans and wildlife[J].Ecological Applications,2010,20(1):16-29.

[28] 李基棕,高颖,杜海燕,等.规模化养猪场环境细菌的调查与分析[J].中国畜牧兽医,2010,37(8):199-203.

[29] Sayah R S,Kaneene J B,Johnson Y,et al.Patterns of antimicrobial resistance observed in Escherichia coli isolates obtained from domestic and wild-animal fecal samples,human septage,and surface water [J].Appl Environ Microbiol,2005,71(3):1394-1404.

[30] 汤电,郭玉芳,王丽华,等.食品动物源大肠杆菌多重耐药性监测[J].中国畜牧兽医,2012,39(12):182-185.

[31] Watkinson A J,Micalizzi G B,Graham G M,et al.Antibiotic-resistant Escherichia coli in wastewaters,surface waters,and oysters from an urban riverine system[J].Appl Environ Microbiol,2007,73(17):5667-5670.

[32] Lupo A,Coyne S,Berendonk T U.Origin and evolution of antibiotic resistance:The common mechanisms of emergence and spread in water bodies[J].Front Microbiol,2012,3:18.

[33] 杨帆.葡萄糖对水源性肠球菌抗生素耐药形成的影响[D].福州:福建农林大学,2008.

[34] 包松英.氮磷营养盐对模型生态系统中粪肠球菌抗生素耐药形成的影响[D].福州:福建农林大学,2013.