地衣芽孢杆菌对发酵床饲养仔猪生长性能、消化酶活性及肠道主要菌群数量的影响

doi:10.16431/j.cnki.1671-7236.2015.04.023

摘要/Abstract

摘要： 本试验旨在研究发酵床饲养模式下日粮中添加地衣芽孢杆菌对仔猪生长性能、胰腺和小肠黏膜消化酶活性及肠道主要菌群数量的影响.选用108头体重13 kg左右的35日龄健康苏钟猪,随机分为3组,每组3个重复,每个重复12头,分别饲喂基础日粮(对照组)、基础日粮+40 mg/kg杆菌肽锌+20 mg/kg硫酸黏杆菌素(抗生素组)、基础日粮+300 mg/kg地衣芽孢杆菌(益生菌组)的试验日粮.预试期7 d,正试期52 d.结果表明:①与对照组相比,日粮中添加地衣芽孢杆菌能够在一定程度上提高仔猪平均日增重、平均日采食量,降低料重比,但各组间差异均不显著(P>0.05).②与对照组相比,日粮中添加地衣芽孢杆菌能够极显著提高仔猪胰腺中淀粉酶活性、十二指肠黏膜中麦芽糖酶活性和空肠黏膜中乳糖酶活性(P<0.01),十二指肠黏膜和空肠黏膜中蔗糖酶活性显著提高(P<0.05).③与对照组相比,日粮中添加地衣芽孢杆菌能够显著提高仔猪盲肠芽孢杆菌数量(P<0.05);乳酸杆菌数量较对照组提高4.09%,大肠杆菌数量较对照组降低4.86%,但均未达到显著水平(P>0.05).综上所述,在本试验条件下,日粮中添加300 mg/kg地衣芽孢杆菌能够提高发酵床饲养仔猪胰腺、小肠黏膜消化酶活性及盲肠芽孢杆菌数量,同时仔猪生长性能也在一定程度上有所改善.

关键词: 地衣芽孢杆菌; 发酵床; 消化酶; 微生物菌群

Abstract: This study investigated the effect of Bacillus licheniformis on growth performance, digestive enzymes activity of pancreas, disaccharidase of intestinal mucosa and the number of intestinal main microbial flora of weaned piglets raised in fermentation bed. One hundred and eight 35-day-old weaned piglets with similar average body weight were randomly divided into three groups with three replicates in each group and twelve piglets in each replicate. The weaned piglets in three groups were fed a basal diet (control group, CT), basal diet+40 mg/kg bacitracin zinc+20 mg/kg colistin sulphate (antibiotic group, ABT) and basal diet+300 mg/kg Bacillus licheniformis (probiotics group, PBT). The pre-test period lasted for 7 days and the trial period lasted for 52 days. The results showed as follows:①Compared with the control group, the supplementation of Bacillus licheniformis could increase ADG, ADFI and decrease F/G to some extent, but there were no significant differences in growth performance among all groups (P>0.05). ②Compared with the control group, the supplementation of Bacillus licheniformis extremely significantly increased the activitiy of amylase in pancreas, the activity of maltase in duodenum mucosa and the activity of lactase in jejunum mucosa (P<0.01), and significantly increased the activity of sucrase in duodenum and jejunum mucosa (P<0.05). ③Compared with the control group, the supplementation of Bacillus licheniformis significantly increased the number of Bacillus in caecum (P<0.05), and the number of Lactobacillus was increased by 4.09%, the number of Escherichia coli was decreased by 4.86%, but the differences were not significant (P>0.05). In conclusion, supplementation of 300 mg/kg Bacillus licheniformis could improve the activity of digestive enzymes in pancreas and intestinal mucosa of piglets, increase the number of Bacillus in caecum, and improve the growth performance to some extent.

Key words: Bacillus licheniformis; fermentation bed; digestive enzymes; microbiology flora

中图分类号:

S828

徐小明, 白建勇, 宦海琳, 闫俊书, 周维仁. 地衣芽孢杆菌对发酵床饲养仔猪生长性能、消化酶活性及肠道主要菌群数量的影响[J]. , 2015, 42(4): 923-928.

XU Xiao-ming, BAI Jian-yong, HUAN Hai-lin, YAN Jun-shu, ZHOU Wei-ren. Effect of Bacillus licheniformis on Growth Performance,Digestive Enzymes and the Number of Intestinal Main Microbial Flora of Piglets Raised in Fermentation Bed[J]. , 2015, 42(4): 923-928.

参考文献

[1] Rolfe R D.The role of probiotic cultures in the control of gastrointestinal health[J].The Journal of Nutrition,2000,130(2S):396-402.
[2] 周继章.抗菌肽抗病的研究进展[J].中国畜牧兽医,2012,39(7):204-208.
[3] 李波,韩文瑜.日粮添加天蚕素抗菌肽对断奶仔猪生产性能、免疫性能及血清生化指标的影响[J].中国畜牧兽医,2014,41(7):99-104.
[4] 代腊,袁超,蒋媛婧,等.大蒜素抗菌特性及其作用机理[J].中国饲料,2012,6:9-11.
[5] 涂兴强.糖萜素、大蒜素在生长育肥猪中的应用研究[D].南宁:广西大学,2013.
[6] 王远孝,李雁,钟翔,等.猪用发酵床的研究与应用[J].家畜生态学报,2007,28(6):139-142.
[7] 王诚,张印,王怀忠,等.发酵床饲养模式对猪舍环境、生长性能、猪肉品质和血液免疫的影响[J].山东农业科学,2009,11:110-112.
[8] Groenestein C M,Van Faassen H G.Volatilization of ammonia,nitrous oxide and nitric oxide in deep-litter systems for fattening pigs[J].Journal of Agricultural Engineering Research,1996,65(4):269-274.
[9] 陈家祥,张仁义,王全溪,等.地衣芽孢杆菌对肉鸡生长性能、抗氧化指标和血液生化指标的影响[J].动物营养学报,2010,22(4):1019-1023.
[10] 杨家军,钱坤,章薇,等.地衣芽孢杆菌对肉鸡生长性能、抗氧化及营养物质代谢的影响研究[J].中国兽医学报,2014,34(5):736-740.
[11] 董晓丽,张乃锋,周盟,等.复合益生菌对断奶仔猪生长性能、粪便微生物和血清指标的影响[J].动物营养学报,2013,25(6):1285-1292.
[12] Krogdahl A,Sell J L.Influence of age on lipase,amylase activities in pancreatic tissue and intestinal contents of young turkeys[J].Poultry Science,1989,68(11):1561-1568.
[13] 王亚芳,肖群平,吕慧源,等.猪复合微生态制剂菌株的筛选及对仔猪生产性能的影响[J].中国畜牧杂志,2014,50(4):58-63.
[14] 刘江坤,芦春莲,李同洲,等.枯草芽孢杆菌(PB6株)制剂对断奶仔猪生产性能的影响[J].畜牧与兽医,2014,46(2):37-40.
[15] 秦枫,潘孝青,李晟,等.发酵床养殖对猪组织器官和血液免疫指标的影响[J].畜牧与兽医,2013,45(10):72-75.
[16] 刘阳.地衣芽孢杆菌KL6对鲤鱼生产性能及消化酶活性的影响[D].天津:天津师范大学,2011.
[17] 姚东林,邹青,刘文斌,等.地衣芽孢杆菌和低聚木糖对草鱼生长性能、肠道菌群和消化酶活性的影响[J].大连海洋大学学报,2014,29(2):136-141.
[18] 杨慧.芽孢杆菌制剂对小鼠黏膜结构及黏膜免疫的影响研究[D].雅安:四川农业大学,2007.
[19] 陈家祥,张仁义,王全溪,等.地衣芽孢杆菌对麻羽肉鸡肠道组织结构及盲肠微生物区系的影响[J].动物营养学报,2010,22(3):757-761.
[20] 槐玉英,纪少丽,梁梓森,等.重组生长因子对早期断奶仔猪小肠黏膜形态和二糖酶活性的影响[J].畜牧与兽医,2013,45(1):72-76.
[21] 银杰.地衣芽孢杆菌对菌群失调大鼠肠道微生态、黏膜结构和免疫细胞的影响[D].武汉:华中农业大学,2009.
[22] 邓文.地衣芽孢杆菌缓解蛋鸡热应激的效果及机制研究[D].北京:中国农业科学院,2011.
[23] Candela M,Perna F,Carnevali P,et al.Interaction of probiotic Lactobacillus and Bifidobacterium strains with human intestinal epithelial cells:Adhesion properties,competition against enteropathogens and modulation of IL-8 production[J].International Food Microbiology,2008,125(3):286-292.
[24] 杨家军,钱坤,章薇,等.地衣芽孢杆菌对肉鸡肠道菌群数量和小肠形态的影响研究[J].中国兽医学报,2014,34(4):664-669.
[25] 孙小沛,杨在宾,李兆勇,等.地衣芽孢杆菌与日粮蛋白水平对肉鸡生产性能、肠道环境及免疫器官指数的影响[J].饲料工业,2014,34(23):40-47.
[26] 张勇军.抗菌肽在断奶仔猪日粮中添加效果的研究[D].长沙:湖南农业大学,2012.
[27] Tam N K,Uyen N Q,Hong H A,et al.The intestinal life cycle of Bacillus subtilis and close relative[J].J Bacteriol,2006,188(7):2692-2700.
[28] 张大伟,王少杰,郝永任,等.芽孢杆菌在肉鸡肠道中的生活状态和分布[A].2006山东饲料科学技术交流大会论文集[C].2006.