中草药泰山磐石散加减方对小鼠肠道微生物菌群的影响

doi:10.16431/j.cnki.1671-7236.2020.08.033

摘要/Abstract

摘要： 为了解中草药泰山磐石散加减方对小鼠肠道微生物菌群的影响，本试验选取45只SPF级昆明小鼠，分为对照A组、试验B组和试验C组。试验B组和C组小鼠分别用0.86 g/mL的中草药泰山磐石散方灌胃0.3和0.4 mL，对照A组灌胃蒸馏水0.4 mL，每天灌胃2次，间隔8 h，连续7 d。第8天采集对照组和试验组小鼠直肠部位粪便样品，从每份样品中提取微生物基因组总DNA，采用Illumina HiSeq测序技术检测样品微生物群落多样性。结果显示，中草药泰山磐石散对小鼠粪便中微生物α多样性指数（Chao1和Shannon）呈波动性变化，试验B和C组与对照A组的Chao1指数差异极显著（P<0.01）；通过检测小鼠对照组与试验组粪便中的微生物，在门水平上，有9大优势菌门，其中与对照A组相比，试验组肠道微生物Proteobacteria、Verrucomicrobia丰度显著降低（P<0.05）；而Saccharibacteria、Tenericutes丰度显著或极显著增加（P<0.05；P<0.01）；在科水平上，有19大优势菌科，其中与对照组相比，试验组肠道微生物Lactobacillaceae、Desulfovibrionaceae、Coriobacteriaceae、Porphyromonadaceae、Family_XIII和Bifidobacteriaceae丰度显著降低（P<0.05）；在属水平上，有18大优势菌属，其中与对照A组相比，试验B、C组肠道微生物Lactobacillus、Desulfovibrio和Enterorhabdus丰度显著降低（P<0.05），而试验C组的肠道微生物Alloprevotella、Bacteroides、Lachnospiraceae_UCG-006和Faecalibaculum丰度显著增加（P<0.05）。本试验中草药泰山磐石散加减方能够有选择性的促进小鼠肠道内Bacteroidetes的生长，从而抑制Proteobacteria有害菌的生长，增强糖和脂肪代谢，最终通过改善肠道微生物多样性的结构，使整个肠道微生物多样性达到一个新的平衡。

关键词: 小鼠; 肠道微生物菌群; Illumina HiSeq测序技术

Abstract: In order to understand the effects of Chinese herbal medicine of Taishan Panshi san on the intestinal microflora in mice.45 SPF Kunming mice were selected and divided into control group A,test group B,and test group C.The mice in test group B and group C were administered with 0.86 g/mL Chinese herbal medicine of Taishan Panshi san for 0.3 and 0.4 mL respectively,while in group A were administrated with 4 mL distilled water,twice a day for 8 hours for 7 days.On the 8th day,feces samples from the rectum were collected from the control and test groups.Microbial genomic DNA was extracted for each sample.Illumina HiSeq sequencing technology were used to detects sample microbial community diversity.In the aspect of α diversity,the Chinese herbal medicine of Taishan Panshi san showed a fluctuant change in the microbial diversity index (Chao1 and Shannon) in the feces of mice,and the difference between the Chao1 index of the experimental group and the control group was extremely significant (P<0.01);In terms of β diversity,detecting the microorganisms in the feces of control and experimental group of mice,level on the phylum,compared with the control group,there were 9 dominant phylum,Proteobacteria and Verrucomicrobia in the experimental group decreased significantly (P<0.05),while Saccharibacteri and Tenericutes in the experimental group increased significantly (P<0.05;P<0.01);At the family level,there were 19 dominant bacteria families,Lactobacillaceae,Desulfovibrionaceae,Coriobactaceae,Porphyromonaceae,Family_XIII and Biofidobactriaceae in the experimental group were significantly lower than those in the control group (P<0.05);At the genus,there were 18 dominant bacteria,among which,Lactobacillilus,Desulfovibrio and Enterhorhabdus were significantly lower in groups B and C than in group A (P<0.05),while Alloprevotella,Bacteroides,Lachnospiraceae_UCG-006 and Faecalibaculum in the experimental C group increased significantly (P<0.05).The Chinese herbal medicine of Taishan Panshi san could selectively promote the growth of Bacteroidetes in the intestinal tract of mice,thereby significantly inhibiting the harmful effects of Proteobacteria,enhance the metabolism of sugar and fat,finally achieve a new balance of intestinal microbial diversity by improving the structure of intestinal microbial diversity.

Key words: mice; intestinal microflora; Illumina HiSeq sequencing technology

中图分类号:

S859.2

买占海, 崔耀成, 沙娅·奴尔兰, 卢亚宾, 姚伟涛, 张耀, 况玲. 中草药泰山磐石散加减方对小鼠肠道微生物菌群的影响[J]. 中国畜牧兽医, 2020, 47(8): 2634-2642.

MAI Zhanhai, CUI Yaocheng, SHAYA·Nuerlan, LU Yabin, YAO Weitao, ZHANG Yao, KUANG Ling. Effect of Chinese Herbal Medicine Taishan Panshi San on Intestinal Microbial Community in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2634-2642.

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