麻叶荨麻醇提液对大鼠离体子宫运动的影响

doi:10.11843/j.issn.1671-7236.2015.03.016

《中国畜牧兽医》---唯一指定的官方网站 ›› 2015, Vol. 42 ›› Issue (3): 613-618.doi: 10.11843/j.issn.1671-7236.2015.03.016

麻叶荨麻醇提液对大鼠离体子宫运动的影响

周玉东, 赵红琼, 张丹, 王丽芳, 张伯伟, 刘璐, 张晓红, 刘朝晖

新疆农业大学动物医学学院, 乌鲁木齐 830052

收稿日期:2014-09-19 出版日期:2015-03-20 发布日期:2015-03-30
通讯作者: 赵红琼 E-mail:zhaohongqiong@sina.com
作者简介:周玉东(1990-),男,新疆伊宁人,硕士生,研究方向:生物活性物质功能,E-mail:348266195@qq.com
基金资助:
国家青年科学基金(31201872);新疆维吾尔自治区普通高校重点学科(基础兽医学);新疆农业大学项目(sysblx016)

Effect of Alcohol Extract of Urtica cannabina on the Motility of Rat Uterus in vitro

ZHOU Yu-dong, ZHAO Hong-qiong, ZHANG Dan, WANG Li-fang, ZHANG Bo-wei, LIU Lu, ZHANG Xiao-hong, LIU Chao-hui

College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China

Received:2014-09-19 Online:2015-03-20 Published:2015-03-30

摘要/Abstract

摘要： 为探究荨麻对子宫运动的影响及其可能机制,本试验用生物信号处理系统测定了浓度递增(0.5%、1.0%、2.0%、4.0%、8.0%和16.0%)的麻叶荨麻(Urtica cannabina,UC)醇提液对未孕大鼠离体子宫收缩频率、收缩张力和收缩幅度的影响,并用特异性M-受体阻断剂硫酸阿托品(atropine,A,2.5 μg/mL)和2.0% UC联合用药,分为3个处理: UC单独处理、UC+A和A+UC,观察UC对子宫收缩的影响是否与M-受体有关.结果显示,在UC浓度递增到4.0%时收缩频率较用药前显著增加(P <0.05);浓度递增到4.0%、8.0%时,收缩幅度较用药前极显著降低(P <0.01);但浓度递增UC对子宫收缩张力影响不显著(P >0.05).一次性加入2.0% UC后子宫收缩张力迅速增加并接近僵直,之后逐渐降低.无论UC+A或A+UC处理都使子宫收缩频率较用药前明显加快,收缩张力极显著增加(P <0.01),但2个联合用药处理组之间差异不显著(P >0.05);UC+A和A+UC处理组收缩张力较UC单独用药组均极显著降低(P <0.01).结果表明,UC可兴奋未孕大鼠离体子宫,A可缓解UC引起的子宫僵直或张力增加,但不能阻断UC引起的子宫收缩频率增加.提示UC对未孕大鼠离体子宫的兴奋作用和激动与M-受体有一定关系,但可能还存在其他机制.

关键词: 麻叶荨麻; 子宫; 离体; 平滑肌

Abstract: To explore the effects of Urtica cannabina (UC) on uterus motility and its possible mechanism,alcohol extracts of UC at increasing doses (0.5%,1.0%,2.0%,4.0%,8.0% and 16.0%) were added to non-pregnant rat uterus in vitro.Changes of contraction frequency,contraction tension and contraction amplitude were recorded by biological signal processing system.Moreover,the combined effects of specific M-receptor antagonist atropine sulfate (A) at the dose of 2.5 μg/mL and UC at a dose of 2.0% on the motility of rat uterus were tested.There were three treatments,UC alone,UC+A and A+UC.The results showed that the contraction frequency was significantly increased when the concentration of UC arrived 4.0% compared with that before treatment (P <0.05);When the concentrations arrived 4.0% and 8.0%,contraction amplitudes of the uterus were extremely significantly decreased compared with that before treatment (P< 0.01);However,the contraction tension was not affected by the increasing concentrations of UC (P >0.05).When 2.0% UC alone treatment,uterus contraction tension increased rapidly and was close to rigidity,and then gradually decreased.Contraction frequencies after UC+A or A+UC treatments were extremely significantly faster than that before treatment (P <0.01),but the difference between the two combined treatments was not significant (P >0.05).Contraction tension of uterus after UC+A and A+UC treatments were extremely significantly reduced compared with that after UC alone treatment (P <0.01).The results indicated that UC could stimulate the contraction of rat uterus partly medicated by M-receptor,whereas there maybe were some other mechanisms involved in the effect.

Key words: Urtica cannabina; uterus; in vitro; smooth muscle

中图分类号:

S853.7

周玉东, 赵红琼, 张丹, 王丽芳, 张伯伟, 刘璐, 张晓红, 刘朝晖. 麻叶荨麻醇提液对大鼠离体子宫运动的影响[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(3): 613-618.

ZHOU Yu-dong, ZHAO Hong-qiong, ZHANG Dan, WANG Li-fang, ZHANG Bo-wei, LIU Lu, ZHANG Xiao-hong, LIU Chao-hui. Effect of Alcohol Extract of Urtica cannabina on the Motility of Rat Uterus in vitro[J]. , 2015, 42(3): 613-618.

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麻叶荨麻醇提液对大鼠离体子宫运动的影响

Effect of Alcohol Extract of Urtica cannabina on the Motility of Rat Uterus in vitro

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