NEAT1在小鼠早期妊娠子宫组织中的表达和调节

doi:10.16431/j.cnki.1671-7236.2021.11.016

摘要/Abstract

摘要： 探讨核旁斑组装转录本1（NEAT1）在小鼠早期妊娠子宫组织中的表达和调节，为揭示NEAT1在小鼠胚胎着床过程中的作用机制提供科学依据。分别构建小鼠早期妊娠、假孕、人工诱导蜕膜化和类固醇激素处理模型，采用RNA原位杂交以及实时荧光定量PCR技术检测NEAT1在上述模型子宫组织的定位与表达。结果显示，NEAT1在小鼠早期妊娠子宫组织中呈现动态变化规律，在妊娠1~5 d的子宫内膜组织和肌层组织中均有表达，且主要表达于子宫内膜腔上皮细胞和腺上皮细胞；在妊娠6~8 d的子宫组织中NEAT1主要表达于蜕膜化细胞，而在孕体组织中未见阳性表达。在小鼠假孕子宫组织中，NEAT1的定位表达与早期妊娠基本一致，在子宫内膜组织和肌层组织中均有表达。在人工诱导蜕膜化模型中，NEAT1主要定位于蜕膜区，且其表达水平与对照组相比极显著上升（P<0.01）。在类固醇激素处理模型中，孕酮（P₄）和雌二醇（E₂）均可显著上调NEAT1的表达水平（P<0.05），且对子宫内膜基质细胞中NEAT1表达上调的作用更加显著。本研究结果表明，NEAT1参与调节小鼠早期妊娠和蜕膜化进程，其表达水平受到类固醇激素的显著上调。

关键词: 核旁斑组装转录本1(NEAT1); 早期妊娠; 子宫组织; 小鼠

Abstract: The test was aimed to study the expression and regulation of nuclear paraspeckle assembly transcript 1 (NEAT1) in uterine tissue during early pregnancy of mice, and provided scientific evidence for revealing the mechanism of NEAT1 in mouse embryo implantation. The models of early pregnancy, pseudopregnancy, artificial decidualization and steroid hormone treatments were constructed respectively, and the expression of NEAT1 in the uterine tissue was detected by RNAscope and Real-time quantitative PCR techniques. The results showed that the expression of NEAT1 was dynamically changing in uterine tissue in early pregnancy. NEAT1 was detected in endometrium and muscular layer on days 1-5 of pregnancy and mainly observed in endometrial luminal and glandular epithelial cells. NEAT1 was highly expressed in decidual cells on days 6-8 of pregnancy. There was no detectable NEAT1 staining at conceptus. Similar with those of early pregnancy, the expression of NEAT1 in pseudopregnant uteri was observed in endometrium and muscular layer. In the artificial decidulization model, NEAT1 was mainly localized in the decidualised cells and the expression level was extremely significant higher than that on the control group (P<0.01). In the steroid hormone treatment model, progesterone (P₄) and estradiol (E₂) could significantly up regulate the expression of NEAT1 (P<0.05), and had a more significant effect on the up regulation of NEAT1 expression in endometrial stromal cells. The above results indicated that NEAT1 was involved in regulating early pregnancy and decidulization processes in mice, and the expression level was mainly regulated by steroid hormones.

Key words: nuclear paraspeckle assembly transcript 1(NEAT1); early pregnancy; uterine tissue; mice

中图分类号:

S857.2

张新艳, 贾燕妮, 蔡芮, 张瑞雪, 靳亚平, 林鹏飞. NEAT1在小鼠早期妊娠子宫组织中的表达和调节[J]. 中国畜牧兽医, 2021, 48(11): 4067-4073.

ZHANG Xinyan, JIA Yanni, CAI Rui, ZHANG Ruixue, JIN Yaping, LIN Pengfei. Expression and Regulation of NEAT1 in Mouse Uterine Tissue During Early Pregnancy[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4067-4073.

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