Abstract: A pair of primer was synthesized based on the published Streptococcus suis type 2 glyceraldehyde-3-phosphate dehydrogenase gene (Gapc) and PCR was applied to detect 10 Streptococcus strains. The amplified products were cloned and sequenced. The results showed that the Gapc gene of 8 Streptococcus strains was detected in all tested strains.The Gapc gene of the above 4 strains were sequenced and analysised by BLAST and DNAStar with pubished Streptococcus Gapc in GenBank. The nucleotide homology of them was 85.3% to 98.3% and the amino acid homology was 87.2% to 99.4%. It had many domains of high antigen index and hydrophilicity and surface probability plot and Gapc gene in Streptococcus was conservative.The results indicated that the Gapc may be applied into clinical prevention for Streptococci infections.

Key words: Streptococcus; Gapc gene; detection; sequence analysis