牛乳头瘤病毒基因1型广西GX01流行株全基因组克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2018.07.001

Abstract

Abstract:

The aim of this study was to investigate the complete genomic sequence,genomic characteristics and genetic variation of bovine papilloma virus genotype 1 (BPV-1) GX01 strain from Guangxi,and the histopathological changes of host was caused by it as well.Firstly,histopathological examination was conducted using the cutaneous papilloma samples of cattle collected from Hezhou city of Guangxi.DNA were extracted and employed as the template of PCR for BPV detection and genotyping using FAP59/FAP64 primers.Based on the complete genomes of the BPV-1 reference strains,specific primer pairs were designed,and the complete genome of the GX01 strain was further amplified and sequenced.Histopathological analysis of cutaneous papilloma showed hyperkeratosis,acanthosis and koilocytosis,which were the typical histopathological characteristics of BPV infection.Genotyping showed GX01 belonged to BPV-1.The full-length genome of GX01 was 7 945 bp,and contained 8 open reading frames (E1,E2,E4,E5,E6,E7,L1 and L2),which matched the structural characteristics of BPV-1.GX01 shared 98.6% to 99.6% homology with the complete genome sequences of BPV-1 reference strains,while 86.9% and 87.2% homology with those of the BPV-2 (M20219.1) and BPV-13 (JQ798171.1) reference strains,respectively.The GX01 strain was the first BPV-1 strain from Guangxi being confirmed and fully sequenced.This study provided theoretical basis for pathogen identification,epidemic regularity,genetic variation,epidemic source tracking and scientific prevention and control of bovine papilloma in Guangxi and China.

Key words: bovine papillomavirus genotype 1(BPV-1); complete genome; sequence analysis

CLC Number:

S852.65

YI Chizhe, SUN Wenchao, ZHENG Min, TU Yuejin, ZHANG Hongyun, BI Jingshan, LIANG Sheng, WEI Xiankai, SU Jiaoxiu, LU Huijun, LI Wenjie, LUO Tingrong. Cloning and Sequence Analysis of the Complete Genome of Bovine Papillomavirus Genotype 1 GX01 Strain from Guangxi[J]. , 2018, 45(7): 1731-1739.

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Cloning and Sequence Analysis of the Complete Genome of Bovine Papillomavirus Genotype 1 GX01 Strain from Guangxi

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