表达耐热肠毒素的重组大肠杆菌对7日龄仔猪肠道形态结构及抗氧化功能的影响

doi:10.16431/j.cnki.1671-7236.2017.09.038

Abstract

Abstract:

This experiment was conducted to investigate the effects of recombinant E.coli expressing heat-stable enterotoxin(STa) on intestinal absorption and barrier function, intestinal morphology and antioxidant capacity of 7 days old piglets. Twenty-four 7 days old piglets were allotted to four treatments:control group (artificial milk), STa group (artificial milk +2×10⁹ CFU E.coli LMG194-pBAD-STa), LMG194 group (artificial milk +2×10⁹ CFU E.coli LMG194), and K88 group (artificial milk +2×10⁹ CFU E.coli K88).The pigs were treated with E.coli on the 5th day and slaughtered on the 7th day. The results showed that, compared with the control group, villus height in jejunum, ileum and duodenum, crypt depth and villus height/crypt depth in duodenum, and small intestine villi surface area were significantly decreased in STa group (P<0.05). The activities of catalase (CAT) in ileum and colon, total nitric oxide synthase (TNOS) in serum, ileum, jejunum and colon, inducible nitric oxide synthase (iNOS) in colon were significantly decreased in STa group (P<0.05). STa group also had a higher levels of malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) in serum (P<0.05). These results suggested that recombinant E.coli expressing STa could lead to intestinal injury and oxidative stress of 7 days old piglets.

Key words: heat-stable enterotoxin(STa); recombinant E.coli; intestinal morphology; antioxidant

CLC Number:

S852.6

LV Yang, ZHANG Lin, LI Xue-ni, ZHAO Di, WU Tao. Effects of Recombinant E. coli Expressing Heat-stable Enterotoxin on Intestinal Morphology and Antioxidant Capacity in Seven Days old Piglets[J]. , 2017, 44(9): 2816-2821.

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Effects of Recombinant E. coli Expressing Heat-stable Enterotoxin on Intestinal Morphology and Antioxidant Capacity in Seven Days old Piglets

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