鸡细小病毒半巢式PCR检测方法的建立及初步应用

doi:10.16431/j.cnki.1671-7236.2017.05.006

Abstract

Abstract:

In order to set up and optimize a semi-nested PCR for rapid detection of chicken parvovirus (ChPV), three specific primers were designed according to conserved sequences of NS 1 gene of ChPV. The specificity and sensitivity of ChPV semi-nested PCR were tested, and the assay was applied to detect 48 clinical samples. The specificity and sensitivity tests showed that this semi-nested PCR was only sensitive to ChPV for amplifying specific band of 186 bp and it could detect 5.62 fg/μL of ChPV DNA, without any sensitivity to other viruses, such as Newcastle disease virus, H9 subtype avian influenza virus, Marek's disease virus, infectious laryngotracheitis virus and infectious bronchitis virus. 48 chicken samples were detected and the positive rate was 16.67% (8/48). The results of our study demonstrated that the optimized semi-nested PCR could be a method that was suitable for clinical detection of ChPV.

Key words: chicken parvovirus; semi-nested PCR; detection

CLC Number:

S858.31

FENG Bin, XIE Zhi-xun, ZHANG Yan-fang, HUANG Jiao-ling, WANG Sheng, FAN Qing, HUANG Li, XIE Li-ji, ZENG Ting-ting, LUO Si-si, DENG Xian-wen, XIE Zhi-qin, LIU Jia-bo, SONG Zhong-bao, LIN Er-ke. Development and Preliminary Application of a Semi-nested PCR Assay for Detection of Chicken Parvovirus[J]. , 2017, 44(5): 1295-1301.

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Development and Preliminary Application of a Semi-nested PCR Assay for Detection of Chicken Parvovirus

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