米非司酮对小鼠精子穿透卵丘细胞层的影响

doi:10.16431/j.cnki.1671-7236.2017.05.003

Abstract

Abstract:

This study was aimed to investigate the effects of RU486 (mifepristone) on sperm penetration through the cumulus cells layer during fertilization in mice. After 20 μg/mL RU486 was added into the capacitation or the sperm/cumulus penetration medium, respectively, the experiments were conducted to evaluate the ratio of sperm acrosome reaction and ability of sperm/cumulus penetration. The results showed that the addition of RU486 significantly suppressed 5 μg/mL P₄-induced acrosome reaction in the capacitated sperm (P <0.01), and decreased sperm penetrating through the cumulus matrix and reaching the oocyte zona pellucid (ZP) and also remarkably reduced the percentage of acrosome-reacted sperm within the oocyte-cumulus complex (OCC)(P <0.01). Compared with the addition of RU486 alone, P₄ did not reverse the inhibitory effects of RU486 on the acrosome reaction of sperm within the OCC, though it still improved sperm penetration through the cumulus matrix and reaching the ZP (P <0.01). Therefore, RU486 could inhibit P₄-induced acrosome reaction and decrease sperm penetration through the cumulus matrix, which suggested that P₄/progesteron receptor (PGR) pathway might be very important for sperm penetration through the cumulus cell layer.

Key words: RU486; P₄; sperm-cumulus interaction; acrosome reaction; sperm

CLC Number:

S814.4

ZHOU Chong, HUANG Li, SHI De-shun, JIANG Jian-rong. Effects of Mifepristone (RU486) on Sperm/Cumulus Penetration in Mice[J]. , 2017, 44(5): 1275-1280.

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Effects of Mifepristone (RU486) on Sperm/Cumulus Penetration in Mice

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