冷冻保存对公猪精子功能的影响

doi:10.16431/j.cnki.1671-7236.2021.05.021

Abstract

Abstract: To investigate the effect of cryopreservation on function of landrace sperm,fresh and frozen semen were selected as research materials.Sperm motility was detected by sperm analyzer.Sperm viability was detected by Trypan blue staining.The cleavage rate and blastocyst rate were measured by in vitro fertilization (IVF).The acrosome integrity rate,mitochondrial permeability transition pore (MPTP) activity,mitochondrial membrane potential (MMP),mitochondrial activity and reactive oxygen species (ROS),and integrity of sperm were measured using different kits.Expression levels of asthenospermia related genes SMCP, TEKT3,DNAH1 and TCTE3 were measured by Real-time quantitative PCR.The results showed that the motility,viability and acrosome integrity rate of frozen sperm were significantly lower than that of fresh sperm (P<0.05).The cleavage rate and blastocyst rate of frozen sperm decreased significantly compared with fresh sperm (P<0.05).The results of mitochondrial function analysis showed that the relative fluorescence units (RFU) value of MPTP,optical density (OD) value of mitochondrial activity and mitochondrial fluorescence ratio of MMP in frozen sperm were significantly lower than that of fresh sperm (P<0.05).The detection of ROS in sperm mitochondria showed that the RUF value of ROS in frozen sperm was significantly higher than that of fresh sperm (P<0.05).DNA integrity test results showed that the sperm tail dragging rate of frozen sperm increased significantly compared with fresh sperm (P<0.05).However,the genes expression of asthenospermia related proteins had no significant difference compared with fresh sperm (P>0.05).In conclusion,cryopreservation decreased the motility,viability,mitochondrial function and DNA integrity of boar sperm,resulting in the decrease of the fertilization ability of frozen sperm.

Key words: boar; frozen semen; sperm mitochondria; sperm DNA integrity; fertilization ability

CLC Number:

S814

HAO Xiaoqiong, WU Zhanying, CUI Yanying, SHI Xiujie. Effects of Cryopreservation on the Function of Boar Sperm[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1699-1706.

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Effects of Cryopreservation on the Function of Boar Sperm

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