内蒙古地区奶牛源致病性沙门氏菌耐药性分析及ESBLs基因检测

doi:10.16431/j.cnki.1671-7236.2015.08.033

Abstract

Abstract: The aim of the present study was to investigate the characteristics of antimicrobial resistance and prevalence of ESBLs genes of Salmonella isolates from dairy cows in Inner Mongolia.Micro-dilution broth method recommended by CLSI was used to determine the MICs of 22 antimicrobial agents commonly employed in veterinary clinic against the 38 Salmonella isolates.PCR analysis was carried out to detect the presence of 8 ESBLs genes mostly observed in animal origin Salmonella in the isolates with resistance to at least one β-lactam antibiotics, and the features of multi-resistance of the isolates were also analyzed.The results showed that most Salmonella isolates were resistant to trimethoprim(97.4%), sulfamethoxazole(94.7%), and to most of the β-lactam, aminoglycosides, chloramphenicol and tetracycline antibiotics with the resistance rate ranging from 40% to 80%, however, all of the isolates were sensitive to fluoroquinolone drugs.76.3%(n＝29) of the isolates were simultaneously resistant to 6 categories antibiotics and the multi-resistance rate was 94.7%. 85.7%(n＝30) of the β-lactam antibiotics resistant isolates were ESBLs genes positive.Of the six genotype of ESBLs genes found in this study, CTX-M (40.0%) was the mostly encountered ESBLs gene, however, the SHV and PSE types gene were absent in the isolates.A total of fifteen genotypes of the ESBLs genes and nine ESBLs genes combinations were observed in this study, and 16 isolates (45.7%) simultaneously contained two or more than two ESBLs genes.It was indicated that the current situation of the resistance of the Salmonella isolates from dairy cows in Inner Mongolia to the commonly used antimicrobial agents in veterinary clinic was serious, and the prevalence pattern of the ESBLs genes in the isolates was complex, suggesting that the irrational employment of the antibiotic in the treatment of animal infectious diseases was the crucial reason causing the antimicrobial resistance of the isolates.

Key words: dairy cows; Salmonella; antimicrobial resistance; ESBLs genes

CLC Number:

S852.61

ZHAO Jun-li, SHI Rui-li, LI Zhi-fang, DING Yue-xia, LI Pei-feng, GUAN Hong. Analysis of Antimicrobial Resistance and Detection of ESBLs Genes of Pathogenic Salmonella Isolates from Dairy Cows in Inner Mongolia[J]. , 2015, 42(8): 2150-2159.

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Analysis of Antimicrobial Resistance and Detection of ESBLs Genes of Pathogenic Salmonella Isolates from Dairy Cows in Inner Mongolia

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