›› 2015, Vol. 42 ›› Issue (3): 701-707.doi: 10.11843/j.issn.1671-7236.2015.03.030

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Isolation,Identification and Drug Resistance Analysis of Escherichia coli in Subclinical Mastitis Cow in Shihezi Region

XU Dan-dan, XU Zhui, QI Xiang-tao, OU Du, YANG Li, QI Ya-yin, YAN Gen-qiang   

  1. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China
  • Received:2014-09-10 Online:2015-03-20 Published:2015-03-30

Abstract: In order to find out the epidemic situation and drug resistance of Escherichia coli in subclinical mastitis cow from large-scale dairy farms in Shihezi region,the method of Lanzhou mastitis test (LMT) was adopted to test 11 large-scale dairy farms around Shihezi.Meanwhile,we separated and identified Escherichia coli from positive milk samples collected under aseptic conditions.Also,drug resistance of these isolates were tested.The results showed that the positive rate of recessive mastitis in large-scale dairy farms in Shihezi area was 44.56%.After separation and identification of 313 milk samples collected under aseptic conditions,108 strains of pathogenic bacteria were isolated,and the separation rate of Escherichia coli was 14.81%.The results of drug sensitivity test showed that 16 stains of Escherichia coli were highly sensitive to ofloxacin,cefazolin and clindamycin,moderately sensitive to gentamicin,ampicillin and streptomycin,drug-resistant to amoxicillin and tetracycline.This study provided scientific basis for prevention and treatment of recessive mastitis caused by Escherichia coli.

Key words: cow recessive mastitis; Escherichia coli; susceptibility test

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