猪繁殖与呼吸综合征病毒荧光定量RT-PCR和RT-LAMP快速检测方法的建立

Abstract

Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) were one of the worldwide causative agent for the disease of pigs and caused heavy losses every year.In this study, a TaqMan-LNA based Real-time RT-PCR assay and a reverse transcription loop-mediated isothermal amplification （RT-LAMP） assay were established.The detection limits were 10²and 10¹ copies/μL for Real-time RT-PCR and RT-LAMP assay， respectively. Two methods both showed good specificity.Futhermore both methods were analysed with clinical samples, and they exhibited high sensitivity and specificity.The Real-time RT-PCR and RT-LAMP assays would provide useful tools for the detection of PRRSV.

Key words: porcine reproductive and respiratory syndrome virus; Real-time RT-PCR; reverse transcription loop-mediated isothermal amplication

References

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Development of Real-time RT-PCR and RT-LAMP for Rapid Detection of Porcine Reproductive and Respiratory Syndrome Virus

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