猪繁殖与呼吸综合征病毒GX1001株和GX1002株全基因组分子特征分析

Abstract

Abstract: To investigate the genomic characteristics of porcine reproductive and respiratory syndrome virus (PRRSV), the full-length genome of PRRSV GX1001 and GX1002 strains from Guangxi province were amplified by RT-PCR, sequenced and analyzed. The sequence data showed that, excluding the poly (A) tail, the genomic sequences of GX1001 and GX1002 were 15329 and 15318 bp in length, respectively. Full-length sequence analysis revealed that GX1001 and GX1002 shared 99.3% nucleotide identity with each other, 84.9% to 99.5% nucleotide identity with representative North American (NA) type isolates, 61.5% to 61.9% nucleotide identity with representative European (EU) type isolates, respectively. Full-length sequence comparisons demonstrated that GX1001 and GX1002 had discontinuous 30 aa deletion in 481th aa and 533th to 561th aa of NSP2 region compared with that of the NA prototype VR-2332, which was considered as the genetic marker of highly pathogenic PRRSV(HP-PRRSV), and had new multiple genomic variations such as mutation, deletion and insertion distributing in different regions. Phylogenetic analysis showed that all the NA type isolates could be divided into four subgroups, and GX1001 and GX1002 belonged to subgroup 4 represented by the highly pathogenic JXA1 strain. The results indicated that GX1001 and GX1002 strains from Guangxi province belonged to HP-PRRSV with new characteristic variations in different regions. This study provided valuable genomic data for further surveillance of HP-PRRSV.

Key words: porcine reproductive and respiratory syndrome virus; highly pathogenic isolate; genomic characteristics; variation analysis

CLC Number:

Q781

LIN Chang-hua, SHI Kai-chuang, TAO Chun-ai, ZHONG Cheng, MO Sheng-lan, LI Gang. Analysis of Genomic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus GX1001 and GX1002 Strains Isolated in Guangxi Province[J]. , 2013, 40(4): 1-9.

References

1 吴忻,韩一超,王娟萍,等. 高致病性猪繁殖与呼吸综合征病毒山西分离株的鉴定及部分Nsp2基因序列分析[J].中国畜牧兽医,2012,39(7):47~52.
2 杨小蓉,荫硕焱,潘梦,等.一株高致病性猪繁殖与呼吸综合征病毒变异毒株的基因组特征[J].畜牧兽医学报, 2012, 43 (2): 263~269.
3 郭宝清,陈章水,刘文兴,等.从疑似PRRS流产胎儿分离PRRSV的研究[J].中国畜禽传染病,1996,87(2): 1~4.
4 Allende R,Kutish G F,Laegreid W,et al.Mutations in the genome of porcine reproductive and respiratory syndrome virus responsible for the attenuation phenotype[J].Arch Virol,2000,145(6):1149~1161.
5 Chen N,Cao Z, Yu X, et al. Emergence of novel European genotype porcine reproductive and respiratory syndrome virus in mainland China[J]. J Gen Virol,2011,92(Pt4):880~892.
6 Collins J,Benfield D A,Christianson W T,et al.Isolation of swine infertibility and respiratory syndrome (SIRS) virus (isolate VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs[J].J Vet Diagn Invest,1992,4(2):117~126.
7 Darwich L,Gimeno M,Sibila M,et al.Genetic and immunobiological diversities of porcine reproductive and respiratory syndrome genotypeⅠstrains[J].Vet Microbiol,2011,150(1~2):49~62.
8 Gao Z Q,Guo X,Yang H C.Genomic characterization of two Chinese isolates of porcine respiratory and reproductive syndrome virus[J].Arch Virol,2004,149(7):1341~1351.
9 Gauger P C,Faaberg K S,Guo B,et al.Genetic and phenotypic characterization of a 2006 United States porcine reproductive and respiratory virus isolate associated with high morbidity and mortality in the field[J].Virus Res,2012,163(1):98~107.
10 Han J,Liu G,Faaberg K S.Identification of nonessential regions of the nsp2 replicase protein of porcine reproductive and respiratory syndrome virus strain VR-2332 for replication in cell culture[J].J Virol,2007,81(18):9878~9890.
11 Han J,Wang Y,Faaberg K S.Complete genome analysis of RFLP 184 isolates of porcine reproductive and respiratory syndrome virus[J].Virus Res,2006,122(1~2):175~182.
12 Li B,Fang L,Guo X,et al.Epidemiology and evolutionary characteristics of the porcine reproductive and respiratory syndrome virus in China between 2006 and 2010[J].J Clin Microbiol,2011,49(9):3175~3183.
13 Liu D,Zhou R,Zhang J,et al. Recombination analyses between two strains of porcine reproductive and respiratory syndrome virus in vivo[J].Virus Res,2011,155(2):473~486.
14 Murtaugh M P,Stadejek T,Abrahante J E,et al.The ever-expanding diversity of porcine reproductive and respiratory syndrome virus[J].Virus Res,2010,154(1~2):18~30.
15 Nam E,Park C K,Kim S H,et al.Complete genomic characterization of a European type 1 porcine reproductive and respiratory syndrome virus isolate in Korea[J].Arch Virol,2009,154(4):629~638.
16 Nelsen C J,Murtaugh M P,Faaberg K S.Porcine reproductive and respiratory syndrome virus comparison: Divergent evolution on two continents[J].J Virol,1999,73(1):270~280.
17 Ostrowski M,Galeota J A,Jar A M,et al.Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain[J].J Virol,2002,76(9):4241~4250.
18 Plagemann P G.The primary GP5 neutralization epitope of North American isolates of porcine reproductive and respiratory syndrome virus[J]. Vet Immunol Immunopathol,2004,102(3):263~275.
19 Ropp S L,Wees C E,Fang Y,et al.Characterization of emerging European-like porcine reproductive and respiratory syndrome virus isolates in the United States[J].J Virol,2004,78(7):3684~3703.
20 Tian K,Yu X,Zhao T,et al.Emergence of fatal PRRSV variants: Unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark[J].PLoS One,2007,2(6):e526.
21 Vashisht K,Goldberg T L,Husmann R J,et al.Identification of immunodominant T-cell epitopes present in glycoprotein 5 of the North American genotype of porcine reproductive and respiratory syndrome virus[J]. Vaccine,2008,26(36):4747~4753.
22 Wensvoot G,Terpstra C,Pol J M,et al.Mystery swine disease in the Netherlands: The isolation of Lelystad virus[J].Vet Q,1991,13(3):121~130.
23 Zhou L,Chen S,Zhang J,et al.Molecular variation analysis of porcine reproductive and respiratory syndrome virus in China[J]. Virus Res,2009a,145(1):97~105.
24 Zhou L,Zhang J,Zeng J,et al. The 30-amino-acid deletion in the Nsp2 of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China is not related to its virulence[J].J Virol,2009b,83(10):5156~5167.
25 Zhou Z,Ni J,Cao Z,et al. The epidemic status and genetic diversity of 14 highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) isolates from China in 2009[J].Vet Microbiol,2011,150(3~4):257~269.
26 Zhou Z,Li X,Liu Q,et al. Complete genome sequence of two novel Chinese virulent porcine reproductive and respiratory syndrome virus variants[J].J Virol,2012,86(11):6373~6374.

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Analysis of Genomic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus GX1001 and GX1002 Strains Isolated in Guangxi Province

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