抗鸡传染性支气管炎病毒中药的筛选

doi:10.16431/j.cnki.1671-7236.2024.03.036

Abstract

Abstract: 【Objective】 This study was aimed to screen the traditional Chinese medicine (TCM) against avian Infectious bronchitis virus (IBV) and verify therapeutic effect,so as to provide a reference for clinical medication.【Method】 The chicken embryo culture method was used to study the anti-IBV screening test which selected 24 single TCM and 6 commercially available compound TCM.The experimental procedure was to inject TCM in SPF chicken embryos first and then inoculate the virus 2 h later or inoculate the virus first and then inject TCM 2 h later.The variance (ANOVA) analysis of chicken embryo weight was performed with SPSS 20.0 software,and Real-time quantitative PCR was used to assist in determining the therapeutic effect.【Result】 ANOVA on the net weight of chicken embryos showed that there was significant difference between Platycodon grandiflorus (Jacq.) A.DC and positive control group (P＜0.05),and there was no significant difference with negative control group(P＞0.05),indicating that Platycodon grandiflorus (Jacq.) A.DC had preventive and curative effects.The viral load in allantoic fluid by Real-time quantitative PCR showed that Platycodon grandiflorus (Jacq.) A.DC,Houttuynia cordata Thunb,Xuanfeibaidu prescription,Gynostemma pentaphyllum (Thunb.) Makino,Taraxacum mongolicum Hand.-Mazz and Glycyrrhiza uralensis Fisch could inhibit the proliferation of IBV, and the inhibitory effect of Platycodon grandiflorus (Jacq.) A.DC was the best.【Conclusion】 Platycodon grandiflorus (Jacq.) A.DC had the remarkably effective for treating IBV,which laid a reference for the in-depth study of the mechanism of Platycodon grandiflorus (Jacq.) A.DC.

Key words: avian Infectious bronchitis virus (IBV); chicken embryo; traditional Chinese medicine; screening

CLC Number:

S852.65⁺9.6

LI Junxian, REN Tao, YANG Jian, GUO Yang, WEN Anlin, OU Deyuan. Screening of Traditional Chinese Medicine for Anti-avian Infectious Bronchitis Virus[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1241-1249.

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Screening of Traditional Chinese Medicine for Anti-avian Infectious Bronchitis Virus

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