2株金黄色葡萄球菌耐药性分析及中药对其部分耐药基因表达的影响

doi:10.16431/j.cnki.1671-7236.2023.12.041

Abstract

Abstract: 【Objective】 The purpose of this experiment was to study and determine the degree of resistance of Staphylococcus aureus to common antibiotics and traditional Chinese medicine,and to explore the influence of traditional Chinese medicine on the expression of resistance genes in Staphylococcus aureus.【Method】 The resistance of Staphylococcus aureus SA1 and SA2 to commonly used antibiotics was detected by Kirby-Bauer method.The minimum inhibitory concentrations (MIC) of 10 traditional Chinese medicines was determined,and the 4 traditional Chinese medicines with the best effect were selected.The strains were added to BHI medium containing 3% glucose at different concentrations of 4 traditional Chinese medicine (0,1/2,1/4 and 1/8 MIC),and the effects of 4 traditional Chinese medicines on the expression of some drug resistance genes in Staphylococcus aureus were detected by Real-time quantitative PCR.【Result】 The results of drug sensitivity test showed that SA1 strain was highly resistant to ampicillin,moderately resistant to ceftazidime,and sensitive to other drugs.SA2 strain had high resistance to ampicillin,penicillin,erythromycin,clindamycin,vancomycin and oxacillin,and sensitive to other drugs.The results of MIC determination of 2 strains of Staphylococcus aureus by 10 kinds of traditional Chinese medicine decoction showed that Coptis chinensis,Cortex Phellodendri chinensis,Galla chinensis and Sanguisorba officinalis L.had the best inhibitory effect on 2 strains.Real-time quantitative PCR results showed,under different liquid concentrations, Galla chinensis significantly decreased the expression of mecA and mecB genes in SA1 strain (P＜0.01). Coptis chinensis and Cortex Phellodendri chinensis significantly decreased mecB gene expression in SA1 strain (P＜0.01). Coptis chinensis and Sanguisorba officinalis L. significantly decreased mecA gene expression in SA2 strain (P＜0.01).【Conclusion】 Traditional Chinese medicine could inhibit Staphylococcus aureus and reduce the expression level of related drug resistance genes to a certain extent,among which Coptis chinensis,Galla chinensis and Sanguisorba officinalis L.have the best effect,followed by Cortex Phellodendri chinensis.The results provided a reference for the prevention and treatment of Staphylococcus aureus with traditional Chinese medicine.

Key words: Staphylococcus aureus; drug resistance; traditional Chinese medicine; resistance gene

CLC Number:

S859.3

ZHANG Yaqian, WANG Yanan, GUO Shangzhao, ZHANG Hongwei, SHI Qiumei, JIANG Hailong, ZHAO Xiyan, LIU Yongbo, GAO Guangping. Drug Resistance Analysis of 2 Staphylococcus aureus Strains and the Influence of Traditional Chinese Medicine on Expression of Some Drug Resistance Genes[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 5204-5212.

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Drug Resistance Analysis of 2 Staphylococcus aureus Strains and the Influence of Traditional Chinese Medicine on Expression of Some Drug Resistance Genes

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