1株马乳酒样乳杆菌的分离鉴定及益生特性研究

doi:10.16431/j.cnki.1671-7236.2023.07.037

Abstract

Abstract: 【Objective】 The aim of this study was to isolate lactic acid bacteria with both antibacterial and antioxidant activities from traditional fermented horse milk wine in Kezhou, Xinjiang, and then obtain excellent strains that could be used for the development of micro-ecological agents.【Method】 MRS plate was used to isolate and culture lactic acid bacteria.Dominant strains were screened, morphological characteristics of bacteria were observed, and molecular identification was conducted by 16S rRNA gene sequence analysis.Then the growth curve, acid production ability, acid resistance, bile salt tolerance and bacteriostatic ability of the strain were studied. In vitro antioxidant function of the strain was evaluated by detecting the hydrogen peroxide resistance, the DPPH free radical scavenging ability, superoxide anion scavenging ability and hydroxyl radical scavenging ability of the strain's fermentation broth, cell-free extract and cell suspension.And the safety of the strain was evaluated by drug sensitivity test and feeding test in mice.【Result】 The results showed that a Gram-positive bacterium with round colony shape, white translucent and irregular edges was screened out and named JK-24.The strain was identified as Lactobacillus kefiranofaciens by 16S rRNA cloning and sequencing.From 6 to 18 h, D₆₀₀_nm value increased rapidly and entered the logarithmic growth phase, and the pH of JK-24 fermentation broth decreased obviously.JK-24 could grow in pH 2.5 to 6.5 and tolerate 0.3% bile salt.The diameters of bacteriostatic rings of fermentation broth against Escherichia coli and Staphylococcus aureus were 15 and 20 mm respectively.It could tolerate 3.0 mmol/L H₂O₂ and promote the growth of the strain at the concentration of 1.0 and 2.0 mmol/L.The DPPH free radical scavenging ability, superoxide anion scavenging ability and hydroxyl radical scavenging ability of the strain's fermentation broth and cell-free extract were significantly higher than those of MRS medium (P<0.05).It was sensitive to 19 antibiotics such as penicillin.Intragastric administration of JK-24 had no significant effect on growth performance and organ index of mice(P>0.05).The hematological parameters were in the normal range, and there were no pathological changes in organs.【Conclusion】 The Lactobacillus kefiranofaciens JK-24 isolated from horse milk wine was safe, non-pathogenic, and had good bacteriostatic and antioxidant properties, which could be used as a strain source of biological feed additives.

Key words: Lactobacillus kefiranofaciens; identification; probiotic properties; safety

CLC Number:

S816.79

ZHOU Chuang, SONG Junya, YE Jing, XUN Jiaqi, GAO Haiping, LIU Yaxin, SAJIDAIMU·Maimaiti, LIN Jiaqi, ZHANG Hua. Isolation, Identification and Probiotic Properties of a Strain of Lactobacillus kefiranofaciens[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2974-2986.

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Isolation, Identification and Probiotic Properties of a Strain of Lactobacillus kefiranofaciens

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