东方欧鳊寄生指环虫种类鉴定及系统发育分析

doi:10.16431/j.cnki.1671-7236.2023.07.027

Abstract

Abstract: 【Objective】 This study was aimed to explore the species and morphological characteristics of the Dactylogyrus parasitized in the Abramis brama orientalis in Irtysh River, and the phylogeny of Dactylogyrus parasitized in Cyprinidae, so as to provide theoretical basis for the phylogeny of Dactylogyrus and its co-evolutionary relationship with host.【Method】 From July 2021 to July 2022, morphological and molecular biological methods were used to identify and phylogenetically analyze Dactylogyrus parasitized in the gills of Abramis brama orientalis.【Result】 According to the morphological observation and chitin structure characteristics, the Dactylogyrus parasitized in the Abramis brama orientalis were identified as Dactylogyrus zandti and Dactylogyrus wunderi, and the structure of their copulatory organ was significantly different.By amplifying the 18S-ITS1-5.8S rDNA and 28S rDNA sequences of two species of Dactylogyrus, and constructing the phylogenetic tree by using Bayesian method (BI) and maximum likelihood method (ML), respectively, the Dactylogyrus zandti and Dactylogyrus wunderi were clustered into one branch, and were located in the same branch as the branchial Dactylogyrus parasitized in the subfamily of Leuciscinae.The phylogenetic trees constructed by different ribosomal sequences were basically the same.Most of the Dactylogyrus parasitized in different subfamily fishes were clustered together, and the Dactylogyrus parasitized in Cyprininae fishes were located at the base of the phylogenetic tree.In the phylogenetic tree constructed by 28S rDNA sequence, the Dactylogyrus parasitized in Cyprininae and Labeoninae fishes were clustered into one branch and located at the base of the phylogenetic tree.【Conclusion】 This experiment described the morphological characteristics of two species of Dactylogyrus parasitized in the Abramis brama orientalis, and supplemented the morphological data of Dactylogyrus.The phylogenetic trees with different ribosomal sequences indicated that there were host transfer and intra-host speciation phenomena in the co-evolution between the Dactylogyrus and its hosts.This study supported Cyprininae fishes as the early hosts of Dactylogyrus, and the phylogeny of Dactylogyrus might be influenced by host specificity and geographical location.

Key words: Abramis brama orientalis; Dactylogyrus; identification; phylogeny

CLC Number:

S852.71

WEI Nianwen, LIU Yanjun, SHI Caixia, LIU Doudou, FENG Longji, ZHANG Li, WANG Jinpu, YUE Cheng, HAO Cuilan. Species Identification and Phylogenetic Analysis of the Parasitic Dactylogyrus of Abramis brama orientalis[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2876-2887.

References

[1] 程姜雯, 潘瑛子, 马幸荣, 等.鲫寄生7种指环虫的形态学和分子鉴定[J].水生生物学报, 2023, 47(2):345-354. CHENG J W, PAN Y Z, MA X R, et al.Morphological and molecular identification of 7 species of Dactylogyrus on gills of the goldfish (Carassius auratus)[J].Acta Hydrobiologica Sinica, 2023, 47(2):345-354.(in Chinese)
[2] ATTIA M M, ABDELSALAM M, ELGENDY M Y, et al.Dactylogyrus extensus and Pseudomonas fluorescens dual infection in farmed common carp (Cyprinus carpio)[J].Microbial Pathogenesis, 2022, 173:105867.
[3] BENOVICS M, FRANCOVÁ K, VOLTA P, et al.Helminth communities of endemic cyprinoids of the Apennine Peninsula, with remarks on ectoparasitic monogeneans, and a description of four new Dactylogyrus Diesing, 1850 species[J].Parasitology, 2021, 148(8):1003-1018.
[4] GALLI P, STRONA G, BENZONI F, et al.Monogenoids from freshwater fish in Italy, with comments on alien species[J].Comparative Parasitology, 2007, 74(2):264-272.
[5] LING F, TU X, HUANG A G, et al.Morphometric and molecular characterization of Dactylogyrus vastator and D.intermedius in goldfish (Carassius auratus)[J].Parasitology Research, 2016, 115(5):1755-1765.
[6] CHENG J, ZOU H, LI M, et al.Morphological and molecular identification of Dactylogyrus gobiocypris (Monogenea:Dactylogyridae) on gills of a model fish, Gobiocypris rarus (Cypriniformes:Gobionidae)[J].Pathogens, 2023, 12(2):206.
[7] 温卫栋, 汪博良, 贾舒安, 等.额尔齐斯河银鲫寄生指环虫18S rDNA序列测定及系统发育研究[J].水生态学杂志, 2015, 36(3):95-100. WEN W D, WANG B L, JIA S A, et al.18S rDNA sequence determination and phylogenetic study of parasitic Dactylogyrus in Carassius auratus gibelio in Ergis River[J].Journal of Hydroecology, 2015, 36(3):95-100.(in Chinese)
[8] 容梦婕, 喀迪尔丁·艾尔肯, 张文润, 等.新疆叶尔羌河流域斑重唇鱼寄生单式指环虫中国——新纪录[J].淡水渔业, 2020, 50(6):46-51. RONG M J, AIERKEN K, ZHANG W R, et al.A new record of Dactylogyrus simplex in gills of Diptychus maculates from Yarkand River basin, China[J].Freshwater Fisheries, 2020, 50(6):46-51.(in Chinese)
[9] ŠIMKOVÁ A, DESDEVISES Y, GELNAR M, et al.Morphometric correlates of host specificity in Dactylogyrus species (Monogenea) parasites of European Cyprinid fish[J].Parasitology, 2001, 123(2):169-177.
[10] ŠIMKOVÁ A, REHULKOVÁ E, CHOUDHURY A, et al.Host-specific parasites reveal the history and biogeographical contacts of their hosts:The Monogenea of Nearctic Cyprinoid fishes[J].Biology, 2022, 11(2):229.
[11] WILSON J R, SAUNDERS R J, HUTSON K S.Parasites of the invasive tilapia Oreochromis mossambicus:Evidence for co-introduction[J].Aquatic Invasions, 2019, 14:332-349.
[12] ŠIMKOVÁ A, VERNEAU O, GELNAR M, et al.Specificity and specialization of congeneric monogeneans parasitizing cyprinid fish[J].Evolution, 2006, 60(5):1023-1037.
[13] BENOVICS M, NEJAT F, ABDOLI A, et al.Molecular and morphological phylogeny of host-specific Dactylogyrus parasites (Monogenea) sheds new light on the puzzling Middle Eastern origin of European and African lineages[J].Parasite Vector, 2021, 14(1):372.
[14] 吴相云, 谢明权, 李安兴.指环虫属的早期辐射及其与宿主鲤科鱼类的协同进化关系[J].动物学报, 2007, 4:651-658. WU X Y, XIE M Q, LI A X.Initial radiation of Dactylogyrus and coevolution with the dactylogyrid-cyprinid association[J].Journal of Zoology, 2007, 4:651-658.(in Chinese)
[15] BENOVICS M, DESDEVISES Y, ŠANDA R, et al.High diversity of fish ectoparasitic monogeneans (Dactylogyrus) in the Iberian Peninsula:A case of adaptive radiation?[J].Parasitology, 2020, 147(4):418-430.
[16] 朱新英, 李政, 周波, 等.新疆额尔齐斯河渔业资源保护与管理的探讨[J].水产学杂志, 2008, 21(1):95-98. ZHU X Y, LI Z, ZHOU B, et al.Discussion on the protection and management of fishery resources in Irtysh River, Xinjiang[J].Chinese Journal of Fisheries, 2008, 21(1):95-98.(in Chinese)
[17] 焦丽, 樊江, 赵江山, 等.额尔齐斯河东方欧鳊(Abramis brama orientalis Berg)寄生虫调查研究[J].新疆农业大学学报, 2010, 33(6):489-491. JIAO L, FAN J, ZHAO J S, et al.Investigation and study on parasites of Abramis brama orientalis Berg in Ergis River[J]. Journal of Xinjiang Agricultural University, 2010, 33(6):489-491.(in Chinese)
[18] DZIKA E, KUSZTAŁA A, KUSZTAŁA M.Parasites of carp bream, Abramis brama, from Lake Jamno, Poland[J].Helminthologia, 2007, 44(4):222-225.
[19] 中国科学院水生生物研究所鱼病研究室.鱼病调查手册[M], 第2版.上海:上海科学技术出版社, 1985. INSTITUTE OF HYDROBIOLOGY, CHINESE ACADEMY OF SCIENCES.Fish Disease Investigation Manual[M].2nd ed.Shanghai:Shanghai Science and Technology Press, 1985.(in Chinese)
[20] KUMAR S, NEI M, DUDLEY J, et al.MEGA:A biologist-centric software for evolutionary analysis of DNA and protein sequences[J].Briefings in Bioinformatics, 2008, 9(4):299-306.
[21] ŠIMKOVÁ A, PLAISANCE L, MATĚJUSOVÁ I, et al.Phylogenetic relationships of the Dactylogyridae Bychowsky, 1933(Monogenea:Dactylogyridea):The need for the systematic revision of the Ancyrocephalinae Bychowsky, 1937[J].Systematic Parasitology, 2003, 54(1):1-11.
[22] HASSOUNA N, MITHOT B, BACHELLERIE J P.The complete nucleotide sequence of mouse 28S rRNA gene.Implications for the process of size increase of the large subunit rRNA in higher eukaryotes[J].Nucleic Acids Research, 1984, 12(8):3563-3583.
[23] ZHANG D, GAO F, JAKOVLI AČG1 I, et al.PhyloSuite:An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies[J].Molecular Ecology Resources, 2020, 20(1):348-355.
[24] KATOH K, STANDLEY D M.MAFFT multiple sequence alignment software version 7:Improvements in performance and usability[J].Molecular Biology and Evolution, 2013, 30(4):772-780.
[25] TALAVERA G, CASTRESANA J.Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments[J].Systematic Biology, 2007, 56(4):564-577.
[26] KALYAANAMOORTHY S, MINH B Q, WONG T K F, et al.ModelFinder:Fast model selection for accurate phylogenetic estimates[J].Nature Methods, 2017, 14(6):587-589.
[27] RONQUIST F, TESLENKO M, VAN DER MARK P, et al.MrBayes 3.2:Efficient Bayesian phylogenetic inference and model choice across a large model space[J].Systematic Biology, 2012, 61(3):539-542.
[28] NGUYEN L T, SCHMIDT H A, VON HAESELER A, et al.IQ-TREE:A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies[J].Molecular Biology and Evolution, 2015, 32(1):268-274.
[29] HAO C L, YUE C, YAO W J, et al.Spatial distribution of Dactylogyrus wunderi Bychowsky on gills of Abramis brama orientalis Berg (Leuciscinae) in Irtysh River, China[J].Chinese Journal of Oceanology and Limnology, 2013, 31(5):979-986.
[30] 张丽霞, 范丽仙, 张东, 等.基于28S rDNA部分序列的指环虫属分子系统分析[J].云南师范大学学报(自然科学版), 2021, 41(2):43-50. ZHANG L X, FAN L X, ZHANG D, et al.Research on phylogeny of genus Dactylogyrus Diesing, 1850 based on 28S rDNA partial sequences[J] Journal of Yunnan Normal University (Natural Science Edition), 2021, 41(2):43-50.(in Chinese)
[31] PALADINI G, SHINN A P, TAYLOR N G H, et al.Geographical distribution of Gyrodactylus salaris Malmberg, 1957(Monogenea, Gyrodactylidae)[J].Parasites & Vectors, 2021, 14(1):34.
[32] KOYUN M.Prevalence and intensity of metazoan parasites of Mesopotamian spiny eel, Mastacembelus mastacembelus (Banks & Solander 1794) according to some parameters of the host, inhabiting in Euphrates-Tigris Basin[J].Biharean Biologist, 2021, 15(2):117-123.
[33] ŠIMKOVÁ A, MORAND S, JOBET E, et al.Molecular phylogeny of congeneric monogenean parasites (Dactylogyrus):A case of intrahost speciation[J].Evolution, 2004, 58(5):1001-1018.
[34] ŠIMKOVÁ A, DESDEVISES Y, GELNAR M, et al.Co-existence of nine gill ectoparasites (Dactylogyrus:Monogenea) parasitising the roach (Rutilus rutilus L.):History and present ecology[J].International Journal for Parasitology, 2000, 30(10):1077-1088.
[35] DEDIC N, VETEŠNÍK L, ŠIMKOVÁ A.Monogeneans in intergeneric hybrids of leuciscid fish:Is parasite infection driven by hybrid heterosis, genetic incompatibilities, or host-parasite coevolutionary interactions?[J].Frontiers in Zoology, 2023, 20(1):5.
[36] SEIDLOVÁ L, BENOVICS M, ŠIMKOVÁ A.Gill monogeneans of neotropical cichlid fish:Diversity, phylogenetic relationships, and host-parasite cophylogenetic associations[J].International Journal for Parasitology, 2022, 52(9):603-615.
[37] DESDEVISES Y, MORAND S, LEGENDRE P.Evolution and determinants of host specificity in the genus Lamellodiscus (Monogenea)[J].Biological Journal of the Linnean Society, 2002, 77(4):431-443.
[38] WANG X Z, GAN X N, LI J B, et al.Cyprinid phylogeny based on bayesian and maximum likelihood analyses of partitioned data:Implications for Cyprinidae systematics[J].Science China Life Sciences, 2012, 55(9):761-773.
[39] BENOVICS M, DESDEVISES Y, ŠANDA R, et al.Cophylogenetic relationships between Dactylogyrus (Monogenea) ectoparasites and endemic cyprinoids of the North-Eastern European peri-Mediterranean region[J].Journal of Zoological Systematics and Evolutionary Research, 2020, 58(1):1-21.
[40] ZHANG D, ZOU H, WU S G, et al.Three new Diplozoidae mitogenomes expose unusual compositional biases within the Monogenea class:Implications for phylogenetic studies[J].BMC Evolutionary Biology, 2018, 18(1):133.
[41] LINDQVIST C, PLAISANCE L, BAKKE T A, et al.Mitochondrial DNA variation of a natural population of Gyrodactylus thymalli (Monogenea) from the type locality River Hnilec, Slovakia[J].Parasitology Research, 2007, 101(5):1439-1442.
[42] 吕晓楠, 徐立蒲, 张文, 等.感染鲤浮肿病毒镜鲤的组织病理变化及病毒分布规律研究[J].中国畜牧兽医, 2022, 49(3):1077-1084. LYU X N, XU L P, ZHANG W, et al.Research on histopathological changes and virus distribution of mirror carp infected with Carp edema virus[J].China Animal Husbandry & Veterinary Medicine, 2022, 49(3):1077-1084.(in Chinese)
[43] 陈怀君, 郭长明, 朱善元, 等.海南罗非鱼源无乳链球菌的分离鉴定及生物学特性研究[J].中国畜牧兽医, 2021, 48(8):3058-3068. CHEN H J, GUO C M, ZHU S Y, et al.Isolation, identification and biological characteristics of Streptococcus agalactiae from Tilapia in Hainan[J].China Animal Husbandry & Veterinary Medicine, 2021, 48(8):3058-3068.(in Chinese)
[44] GUPTA G, CHATTERJEE A, KUMAR M, et al.Efficacy of single and multiple doses of fenbendazole against gill parasites (Dactylogyrus sp.) of Labeo rohita (Hamilton, 1822) and its physio-metabolic effects on the fish[J].Aquaculture Research, 2020, 51(3):1190-1199.

Species Identification and Phylogenetic Analysis of the Parasitic Dactylogyrus of Abramis brama orientalis

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