LPS诱导马冈鹅法氏囊损伤相关基因的筛选

doi:10.16431/j.cnki.1671-7236.2023.07.005

Abstract

Abstract: 【Objective】 The purpose of this experiment was to study the effects of lipopolysaccharide (LPS) on tissue injury and oxidative stress of bursae of Fabricius of Magang geese and to explore its regulatory mechanism.【Method】 One hundred 1-day-old Magang geese (half female and half male) were randomly divided into control and LPS group with 5 replicates in each group and 10 geese in each replicate.The goslings in LPS group were injected intraperitoneally with 2 mg/kg LPS at 24, 26 and 28 days, respectively, while the control group was injected with the same amount of saline.After 1 hour of injection at 28 days, the bursa of Fabricius was collected aseptically for histological observation and oxidative stress index detection and transcriptome library construction.Candidate genes related to LPS-induced bursa of Fabricius injury were screened by differentially expressed gene identification, GO function and KEGG pathway enrichment, and protein interaction network analysis.Ten differentially expressed genes were randomly selected and the expressions were verified by Real-time quantitative PCR.【Result】 Histological observation of bursae of Fabricius of Magang goose showed that the structural integrity of bursae of Fabricius was significantly decreased, the cell arrangement in vesicles was disordered and the number of vacuoles increased in LPS group.Compared with the control group, the activity of glutathione peroxidase (GSH-Px) of bursae of Fabricius was significantly decreased in LPS group (P <0.05).A total of 507 differentially expressed genes were obtained in LPS group, of which 277 genes were up-regulated and 230 genes were down-regulated.GO function and KEGG pathway enrichment analysis showed that differentially expressed genes were mainly concentrated in items related to inflammation and immune response, and related to Cytokine-cytokine receptor interaction pathway and Notch and Hedgehog signaling pathways. A total of 27 differentially expressed genes, including TNF superfamily member 10 (TNFSF10), CREB-binding protein (CREBBP), paracentric substance 1 (PCM1), protein tyrosine phosphatase non-receptor type 6 (PTPN6), were screened by binding protein cooperative network analysis.The results of Real-time quantitative PCR showed that the transcriptome sequencing results were accurate and reliable.【Conclusion】 This study explored the differential expression genes in bursa of Fabricius in Magang goose before and after LPS-induced, and identified the Cytokine-cytokine receptor interaction, Notch and Hedgehog signaling pathways and related genes such as TNFSF10, CREBBP and PTPN6 affecting bursa of Fabricius injury, which provide theoretical reference for understanding the molecular mechanism of LPS-induced bursae of Fabricius injury in geese.

Key words: lipopolysaccharide; Magang goose; bursa of Fabricius injury; transcriptome sequencing

CLC Number:

S835

YU Ziyu, ZHANG Bingqi, CAI Yuanrong, HONG Longsheng, LI Wanyan, CAO Nan, HUANG Yunmao, XU Danning, TIAN Yunbo, CHEN Wenbin, LI Bingxin. Screening of Genes Related to Bursa of Fabricius Injury Induced by LPS in Magang Geese[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2639-2650.

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Screening of Genes Related to Bursa of Fabricius Injury Induced by LPS in Magang Geese

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