蚯蚓液对105~175日龄蛋鸡产蛋性能、血清生化指标、抗氧化指标及免疫相关指标的影响

doi:10.16431/j.cnki.1671-7236.2023.05.017

Abstract

Abstract: 【Objective】 This experiment was aimed to study the effects of earthworm hydrolysate on laying performance,serum biochemical indexes,antioxidant indexes and immune-related indexes of laying hens aged 105 to 175 days,provide data reference for the rational application of earthworm in animal husbandry.【Method】 A total of 720 healthy 105-day-old Hy-line laying hens with similar body weight were randomly divided into 4 groups with 6 replicates per group and 30 hens per replicate.Hens in control group were fed a basal diet,and hens in experimental groups were fed a basal diet supplemented with 1%,2% and 3% earthworm hydrolysate,respectively.The pre-test lasted for 15 days and the positive test lasted for 55 days.During the trial period,daily feed intake,egg production and egg weight were recorded in each replicate.On the 55th day of the trial period,6 eggs were randomly selected from each replicate for egg quality determination,and 6 laying hens were randomly selected from each replicate to collect blood for the determination of serum biochemical indices,serum antioxidant indices and serum immune indices.【Result】 Compared with control group,the differences in the egg laying performance and egg quality index of each test group were not significant (P＞0.05).The content of uric acid was significantly lower, the total antioxidant capacity (T-AOC) were significantly higher of 1% and 3% earthworm hydrolysate groups than that in control group (P＜0.05). The activities of catalase (CAT) and superoxide dismutase (SOD) were significantly higher, the contents of malondialdehyde (MDA), immunoglobulin G (IgG) and interleukin-2 (IL-2) in 1%,2% and 3% earthworm hydrolysate groups were significantly lower than those in control group (P＜0.05).The levels of tumor necrosis factor α(TNF-α) and IL-17 in 1% and 3% earthworm hydrolysate groups were extremely significantly lower than those in control group (P＜0.01).【Conclusion】 Earthworm hydrolysate could promote the excretion of uric acid in 105-175 days old laying hens,enhance the antioxidant capacity and immunity of laying hens,and it was advisable to add 1%.

Key words: earthworm hydrolysate; laying hens; laying performance; immunity; oxidation resistance

CLC Number:

S816.48

XIE Hualiang, LEI Xiaowen, LI Jianjun, LIU Zhenni, ZHONG Yunping, TAN Donghai, ZHANG Qiang. Effects of Earthworm Hydrolysate on Laying Performance,Serum Biochemical Indices,Antioxidant Indices and Immune-related Indices of Laying Hens Aged 105 to 175 Days[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1899-1906.

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Effects of Earthworm Hydrolysate on Laying Performance,Serum Biochemical Indices,Antioxidant Indices and Immune-related Indices of Laying Hens Aged 105 to 175 Days

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