猪SGK家族基因的生物信息学分析及其在猪脂肪组织和细胞中的表达

doi:10.16431/j.cnki.1671-7236.2022.09.005

Abstract

Abstract: 【Objective】 This study was aimed to amplify porcine serum and glucocorticoid-inducible kinase (SGK) family genes and analysis their bioinformatics, as well as to explore their expression patterns in porcine adipose tissues and adipocytes.【Method】 SGK family genes were amplified by PCR using the cDNA of Tibetan pig adipocytes.The physicochemical properties and subcellular localization of the encoded proteins were predicted by online tools.The phylogenetic tree was constructed by Mega X software.The tissues from heart, liver, spleen, kidney, lung, back muscle, leg muscle, cervical fat, backfat, inguinal fat, and perirenal fat of 30-day-old Bama pigs and the inguinal adipose tissues of 7-day-old and 4-month-old Bama pigs were collected.The expressions of SGK family genes in these tissues were detected by Real-time quantitative PCR.The 30-day-old porcine inguinal adipose tissues were collected and stromal vascular fraction (SVF) cells were isolated and differentiated into white adipocytes.The expressions of SGK family genes and adipocyte differentiation marker genes CCAAT/enhance binding protein alpha (C/EBPα) and peroxisome proliferator activated receptor gama (PPARγ) in adipocytes were detected by Real-time quantitative PCR.【Result】 The sequence length of the CDS regions of SGK1, SGK2 and SGK3 genes were 1 296, 1 104 and 1 473 bp, encoding 431, 367 and 490 amino acids, respectively.The SGK1 and SGK2 were localized in cytoplasm, and the SGK3 was localized in nucleus.All three SGK proteins were hydrophilic proteins with the same structural domains and conserved motifs of their encoded proteins.Phylogenetic tree results indicated that pigs were most closely related to cattles.SGK1 and SGK3 genes were widely expressed in heart, liver, spleen, lung, kidney, various muscles and adipose tissues.SGK2 gene was highly expressed in adipose tissues of the cervical fat, backfat, inguinal fat and perirenal fat.The expression of SGK1 and SGK2 genes were extremely significantly higher in adipose tissues of 7-day-old than 4-month-old pigs (P<0.01), and there was no significant difference in the expression of SGK3 gene between 4-month-old and 7-day-old pigs (P>0.05).The expression of SGK3 gene was lower than that of SGK1 and SGK2 genes.Compared with undifferentiated adipocytes, the expression of SGK1 and SGK2 genes were extremely significantly up-regulated in differentiated adipocytes (P<0.01), and the expression of SGK1 gene was higher than that of SGK2 gene, while the expression of SGK3 gene did not change significantly (P>0.05).【Conclusion】 SGK family proteins had conserved structural domains and might play similar functions.SGK1 and SGK2 might be involved in regulating the differentiation of porcine adipocytes, which laid a certain theoretical foundation for exploring the molecular mechanism of porcine fat deposition.

Key words: pigs; SGK family genes; fat deposition; expression analysis

CLC Number:

S829.9

CHEN Chuanhe, LIU Jiali, ZHANG Lilan, ZHAO Ying, TAO Cong. Bioinformatics Analysis of Porcine SGK Family Genes and Their Expression in Porcine Adipose Tissues and Adipocytes[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3310-3320.

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Bioinformatics Analysis of Porcine SGK Family Genes and Their Expression in Porcine Adipose Tissues and Adipocytes

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