槲皮素-替米考星混合物纳米粒的制备及对金黄色葡萄球菌小菌落变异株抗菌活性研究

doi:10.16431/j.cnki.1671-7236.2022.04.029

Abstract

Abstract: 【Objective】 Quercetin-tilmicosin mixture nanoparticles was prepared for the treatment of Staphylococcus aureus small colony variants (SASCVs) infection,and its antibacterial activity against SASCVs strain was evaluated.【Method】 The dosage of quercetin (0.05,0.10,0.15 and 0.20 g) and tilmicosin API (0.2,0.3,0.4 and 0.5 g),rotating speed (500,1 000 and 1 500 r/min),reaction time (0.5,1.0 and 2.0 h),reaction temperature (25,50 and 75 ℃) and solvent concentration (5.0%,7.5% and 10.0%) were determined to screen the optimal formula and preparation conditions of quercetin-tilmicosin mixture nanoparticles.The prepared quercetin-tilmicosin mixture nanoparticles were characterized by micro-morphology,sedimentation volume ratio,re-dispersibility,particle size and Zeta potential.The antibacterial activities of quercetin-tilmicosin mixture nanoparticles and commercial tilmicosin solution against SASCVs strains were compared by agar diffusion method,micro broth dilution method and in vitro sterilization curve,and the advantages of quercetin-tilmicosin mixture nanoparticles were evaluated.【Result】 Quercetin-tilmicosin mixture nanoparticles were the best when tilmicosin was 0.4 g,quercetin was 0.1 g,solvent was 20 mL of 7.5% polyethylene glycol solution,rotating speed was 1 000 r/min,reaction time was 1 h and reaction temperature was 50 ℃,respectively.Its appearance properties of formulation showed no precipitate and floccule,and the color was clear.Its micro-morphology showed that the mixture nanoparticles were evenly distributed and the particle size was the same.The sedimentation volume ratio was 1,and the re-dispersibility was good.The average particle size was 741.9 nm;Zeta potential was -7.69 mV.The inhibition zone diameters of tilmicosin standard,quercetin-tilmicosin mixture nanoparticles and commercial tilmicosin solution were 2.54,1.51 and 1.38 cm,respectively,and the minimum inhibitory concentrations were 1,1 and 2 μg/mL,respectively.The in vitro killing curves showed that quercetin-tilmicosin mixture nanoparticles against SASCVs strains showed obvious concentration-dependent.【Conclusion】 The quercetin-tilmicosin mixture nanoparticles prepared in this study had strong bactericidal ability against SASCVs strains,and could provide basic research data for the treatment of dairy cow mastitis caused by SASCVs strain.

Key words: quercetin; tilmicosin; mixture nanoparticles; Staphylococcus aureus small colony variants; antibacterial activity

CLC Number:

S859.7

LIU Jinhuan, ZHANG Shanling, SONG Wei, LUO Wanhe, CHEN Wei. Preparation of Quercetin-tilmicosin Mixture Nanoparticles and Its Antibacterial Activity Against Small Colony Variant Strains of Staphylococcus aureus[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1470-1478.

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Preparation of Quercetin-tilmicosin Mixture Nanoparticles and Its Antibacterial Activity Against Small Colony Variant Strains of Staphylococcus aureus

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