猪骨骼肌纤维类型和能量代谢相关基因的表达特性研究

doi:10.16431/j.cnki.1671-7236.2022.04.003

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the expression characteristics of myofiber types and energy metabolism related genes in Mashen pigs (MS),Jinfen White pigs (JFW) and Duroc×Landrace×Large (DLY) pigs.【Method】Six-month-old MS,JFW and DLY pigs were slaughtered,and the longissimus dorsi muscle (LD) and extensor digitorum longus muscle (EDL) were collected.HE staining was used to detect the diameter,density and cross-sectional area of myofiber.The expressions of Ⅰ,Ⅱa,Ⅱb and Ⅱx type myofiber marker genes (MYH7,MYH2,MYH4 and MYH1),mitochondrial activity related genes (ATP5A1,PGC1-α and PPARβ),electron transport chain related genes (UQCRC2,SDHA and NDUFA9) and glycolysis related genes (LDHA,LDHB and GK) were detected by Real-time quantitative PCR.【Result】 The diameter and cross-sectional area of longissimus dorsi muscle in MS pigs were significantly lower than those in JFW and DLY pigs,while the density was significantly higher than those in JFW and DLY pigs (P<0.05).The diameter and cross-sectional area of extensor digitorum longus muscle in MS and JFW pigs were significantly lower than those in DLY pigs,while the density was significantly higher than those in DLY pigs (P<0.05).The expression of MYH7 gene in longissimus dorsi muscle was the highest in MS pigs and the lowest in JFW pigs.The expression of MYH7 gene in extensor digitorum longus muscle of MS and JFW pigs were significantly higher than those of DLY pigs (P<0.05).The expression of MYH2 gene in longissimus dorsi muscle and extensor digitorum longus muscle of MS pigs were significantly higher than those of JFW and DLY pigs,and the expression of extensor digitorum longus muscle in JFW pigs was significantly higher than that in DLY pigs (P<0.05).The expression of MYH4 gene in longissimus dorsi muscle of MS pigs was significantly lower than those of JFW and DLY pigs,and the expression of extensor digitorum longus muscle in MS and JFW pigs were significantly lower than those in DLY pigs (P<0.05).The expression of ATP5A1 and PGC1-α genes in longissimus dorsi muscle of MS pigs were significantly lower than those of JFW and DLY pigs,and the expression of PGC1-α and PPARβ genes in JFW pigs were significantly lower than those in DLY pigs (P<0.05).The expression of ATP5A1,PGC1-α and PPARβ genes in extensor digitorum longus muscle of MS and JFW pigs were significantly lower than those of DLY pigs (P<0.05).The expression of UQCRC2,SDHA and NDUFA9 genes in longissimus dorsi muscle of JFW pigs were significantly lower than those of DLY and MS pigs (P<0.05).The expression of UQCRC2,SDHA and NDUFA9 genes in extensor digitorum longus muscle of JFW and MS pigs were significantly lower than those of DLY pigs,and the expression of UQCRC2 and SDHA genes in MS pigs were significantly lower than those in JFW pigs (P<0.05).The expression of LDHA,LDHB and GK genes in longissimus dorsi muscle and extensor digitorum longus muscle of JFW and MS pigs were significantly lower than those of DLY pigs (P<0.05).【Conclusion】 The diameter and cross-sectional area of skeletal muscle in MS pigs were smaller,and the expression of Ⅰ and Ⅱa myofiber marker genes was higher.The expression of Ⅱb myofiber marker gene,oxidative phosphorylation and glycolysis related genes in DLY pigs was higher.

Key words: pigs; myofiber types; oxidative phosphorylation; glycolysis

CLC Number:

Q786

ZHANG Yanwei, CAI Chunbo, SUN Di, AN Jiaqi, HUANG Xiaoyu, NIU Jin, CUI Zixu, GAO Pengfei, GUO Xiaohong, LI Bugao, CAO Guoqing. Study on Expression Characteristics of Myofiber Types and Energy Metabolism Related Genes in Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1223-1232.

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Study on Expression Characteristics of Myofiber Types and Energy Metabolism Related Genes in Pigs

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