藏鸡与白羽肉鸡垂体转录组差异表达研究

doi:10.16431/j.cnki.1671-7236.2021.06.006

Abstract

Abstract: The purpose of this study was to explore the differentially expressed genes (DEGs) and signaling pathways related to growth and development of chicken in pituitary by transcriptome sequencing and bioinformatics analysis.Three 42-day-old healthy Tibetan chickens and White-feather broilers were selected,respectively.The pituitary transcriptome of the 2 chicken breeds was analyzed by Illumina HiSeq 2000 sequencing system.The DEGs were annotated and enriched by GO and KEGG database.The relative expression of randomly selected DEGs were verified by Real-time quantitative PCR.The results showed that 126 094 302 and 125 666 442 clean reads were obtained from Tibetan chicken and White-feather broiler,respectively.Compared with White-feather broilers,there were 392 DEGs in Tibetan chickens pituitary,of which 196 up-regulated genes and 196 down-regulated gene (P<0.05),including growth hormone gene (GH),growth hormone releasing hormone receptor gene (GHRHR) and insulin-like growth factor 2 mRNA binding protein 1 gene (IGF2BP1).GO and KEGG enrichment analysis showed that the DEGs were mainly involved in the pathways related to cell communication,such as cell adhesion molecules and neuroactive ligand-receptor interaction signaling pathways.GH,GHRHR and IGF2BP1 genes were also significantly enriched in neuroactive ligand-receptor interaction signaling pathway.The results of Real-time quantitative PCR showed that the accuracy and reliability of the transcriptome sequencing.The research revealed the key candidate genes and signal pathways for the difference in growth and development between Tibetan chicken and White-feather broiler,and provided a theoretical basis for further understanding of the regulatory mechanisms for growth and development of pituitary.

Key words: Tibetan chicken; White-feathered broiler; transcriptome sequencing; pituitary; growth and development

CLC Number:

S831.2

WANG Hongling, WANG Yingming, LIANG Cheng, TANG Hui, LI Zhixiong, WU Jinbo. Differential Characterization of the Pituitary Transcriptome in Tibetan Chicken and White-feather Broiler[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 1939-1947.

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Differential Characterization of the Pituitary Transcriptome in Tibetan Chicken and White-feather Broiler

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