日粮粗蛋白质水平对伊犁鹅产蛋规律、繁殖激素分泌及相关基因mRNA表达的影响

doi:10.16431/j.cnki.1671-7236.2020.06.011

Abstract

Abstract: This experiment was conducted to study the effects of dietary crude protein level on laying raws,reproductive hormone concentrations and related gene mRNA expression in reproductive axis of Yili geese.200 Yili geese aged 3 years old with similar body weight and good health were selected,and were randomly divided into 4 groups with 10 replications.The crude protein level of control group and male geese (goose field self-administered) was 11.20%.The experimental groups fed the test diets with crude protein levels of 13.86%,15.20% and 16.48%,respectively.The nutritional levels of other diets remained the same.The pre-trial period was 1 week,the formal period was 9 weeks.The results showed that:①The egg production rate of Yili geese showed fluctuating changes during the trial period.The egg production rate of Yili geese in control group gradually increased in the first to second weeks,and slightly increased in the fourth to fifth weeks,the other stages showed a downward trend.The egg production rate of Yili geese in each experimental group significantly increased during the first to second and the fourth to fifth weeks,and reached the peak of egg production at the fifth week,and then gradually declined.The broodiness rate of 15.20% crude protein group in Yili geese was significantly lower than that of 11.20% and 13.86% crude protein groups.②Compared with control group,the serum GnRH content of 13.86% crude protein group were extremely significantly increased (P<0.01),and the serum E₂ and LH contents significantly increased (P<0.05).The serum GnRH,E₂ and LH content of 15.20% and 16.48% crude protein groups were extremely significantly increased (P<0.01),and the serum FSH content was significantly increased (P<0.05).The serum PRL concentration of each test group was significantly lower than that of control group (P<0.05).③Compared with control group,the GnRH,LHR mRNA relative expression of hypothalamus in 15.20% crude protein group were significantly increased (P<0.05),and the PRL and PRLR mRNA relative expression was significantly decreased (P<0.05).The LH mRNA relative expression in pituitary was significantly increased (P<0.05).The LH and ESR2 mRNA relative expression in ovary were significantly or extremely significantly increased (P<0.05 or P<0.01).In summary,based on the comprehensive evaluation of the effects of dietary crude protein level on the laying rate,broodiness rate,serum reproductive hormone concentration and the related gene mRNA expression in reproductive axis of Yili geese.It suggested the suitable crude protein level of the diet in Yili geese was 15.20% during laying period.

Key words: crude protein; Yili geese; laying raws; reproductive hormone; mRNA

CLC Number:

S835

ZHAO Xiaoyu, SHAERSHANBIEKE Abudilida, MA Chen, WU Yingping, WANG Junhua, LI Haiying. Effects of Dietary Crude Protein Level on Laying Raws,Reproductive Hormone Concentrations and Related Gene mRNA Expression in Reproductive Axis of Yili Geese[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1729-1738.

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Effects of Dietary Crude Protein Level on Laying Raws,Reproductive Hormone Concentrations and Related Gene mRNA Expression in Reproductive Axis of Yili Geese

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