大肠杆菌转座子及整合子携带类型与其多重耐药谱型相关性的研究

doi:10.16431/j.cnki.1671-7236.2020.05.030

Abstract

Abstract: To investigate the correlation between transposon and integron carrying and multiple drug resistance of Escherichia coli,259 strains of Escherichia coli were isolated from 12 large-scale pig farms by collecting anal swabs.Minimum inhibitory concentration (MIC) values of isolated strains against 7 classes (16 kinds) of antibacterial drugs were measured by microbroth dilution method,and the carrying of transposons and integrons was detected by PCR.The results showed that the tested strains showed different degrees of resistance to 7 classes (16 kinds) of antibacterial drugs.It was highly resistant to spectinomycin,gentamicin and tetracycline,and the drug resistance multiple of MIC₉₀ was 32 times.It was sensitive to imipenem and polymyxin B,and the drug resistance multiple of MIC₉₀ was 2 times,while the resistance of other drugs was between them.The multiple drug resistance up to 7 times,2 times resistant up to 100%.There were 14 types of multidrug resistance spectrum,mainly including β-lactams+aminoglycosides+tetracyclines+amidoalcohols+sulfonamides+fluoroquinolones,β-lactams+aminoglycosides+tetracyclines+amidoalcohols+sulfonamides+fluoroquinolones+peptides,accounting for 56.97%.The detection rate of transposons and integrons was higher.There were 20 types of transposon and integron phenotypes.Among them,the combination types of IScp1+IS26+tnpA+tnp513+intI1 and IS26+tnpA+tnp513+intI1 accounted for the most,48.48% and 26.06%,respectively.The results showed that insertion element IScp1 was significantly correlated with β-lactam drug resistance (P<0.05,P<0.01).The insertion element IS26 was extremely significantly correlated with ampicillin resistance (P<0.01) and significantly correlated with sulfadioxazole resistance (P<0.05).Integron intI1 was extremely significantly correlated with ampicillin resistance (P<0.01),IntI2 was extremely significantly correlated with cefetadime resistance (P<0.01),and significantly correlated with cefetifur,enoxacin and imipenem resistance (P<0.05).The dominant combination type IScp1+IS26+tnpA+tnp513+intI1 was extremely significantly correlated with the drug resistance of compound sulfamethoxazole (P<0.01).The combination type of IS26+tnpA+tnp513+intI1 was extremely significantly correlated with the drug resistance of compound sulfamethoxazole (P<0.05) and was extremely significantly correlated with ceftifur resistance (P<0.01),but it was negatively correlated with gentamicin resistance (P<0.05).The multidrug resistance of the tested strains to β-lactams,fluoroquinolones,sulfonamide and aminoglycosides were significantly or extremely significantly correlated with transposon and integron carrying type.

Key words: Escherichia coli; transposon; integron; drug resistance

CLC Number:

S859.7

LIN Xi, LYU Shiming, TAN Aijuan, LUO Zhiqian, YANG Ruizhi, ZHENG Ruqing, ZHOU Ling. Correlation Between Transposon and Integron Carrying Type and Multidrug Resistance Spectrum of Escherichia coli[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1547-1559.

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Correlation Between Transposon and Integron Carrying Type and Multidrug Resistance Spectrum of Escherichia coli

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