中药王不留行增乳活性单体及催乳素对奶牛乳腺上皮细胞特异性miRNA的影响

›› 2010, Vol. 37 ›› Issue (8): 230-232.

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Influence on the Specific microRNAs in Bovine Mammary Gland Epithelial Cells by Semen Vaccariae Active Monomer and Prolactin

WAN Zhong-ying, TONG Hui-li, LI Qing-zhang, GAO Xue-jun

（Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University,Harbin 150030, China）

Received:1900-01-01 Revised:1900-01-01 Online:2010-08-20 Published:2010-08-20

Abstract

Abstract: The objective of this research is to find the influence on specific microRNAs in bovine mammary gland epithelial cells by Semen vaccariae active isomer and prolactin. Quantitative real-time PCR was used to detect the expression changes of microRNAs (21, 125b, 143 and 195) in the mammary gland epithelial cells with dibutyl phthalate (DBP) and prolactin (PRL) at different time. The results showed that both DBP and PRL could repress the expression of miRNA-125b, miRNA-143 and miRNA-195 in mammary gland epithelial cell; DBP could repress the expression of miRNA-21,while the influence of PRL on miRNA-21 was not certain. It is the first time to demonstrate that the expression of the microRNAs can be changed by Semen vaccariae active isomer （DBP）and prolactin.

Key words: Semen vaccariae; prolactin; dairy cow mammary gland epithelial cell; dibutyl phthalate; microRNA

CLC Number:

S853.7

WAN Zhong-ying;TONG Hui-li;LI Qing-zhang;GAO Xue-jun. Influence on the Specific microRNAs in Bovine Mammary Gland Epithelial Cells by Semen Vaccariae Active Monomer and Prolactin[J]. , 2010, 37(8): 230-232.

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Influence on the Specific microRNAs in Bovine Mammary Gland Epithelial Cells by Semen Vaccariae Active Monomer and Prolactin

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