Abstract: microRNA played an important role in the process of the animals skin development and their morphogenetic process in previous studies. The database imperfect was still the main reason for limiting its function studies in the study about the microRNA from the goat skin. Our research analyzed the 5.40% no annotation reads from the high-throughput sequencing total reads, reference the goat genomics gene, using the Mireap software to analyze the data, and analyzed the data including new microRNA length, expression, genomics location, predict the target gene and the KEGG pathway. Our research got 28 new microRNA, and their length were equal with the known microRNA in the goat and the cow. Their expression statistics showed that, there were 4 new microRNA their expression over the 100, the highest was the Novel-15, its expression got 555;There were 19 new microRNAs their expression between 10 to 100;There were 5 new microRNA their expression lower 10.We found the new microRNA evenly distributed over the goat chromosome by studying genomics location, exactly locate 2 microRNA on the number 5, 6, 7, 19 and X chromosome respectively. Predicted and analyzed the target gene data we got 6 519 target gene. Analyzed the KEGG pathway showed that, locate the 3 552 target gene on the 51 pathway, the three former pathway were Metabolic pathways, Pathways in cancer and MAPK signaling pathway. Our research found more new goat microRNA, had laid the foundation about the study of the goat skin development regulate.

Key words: goat; microRNA; skin; MAPK