猪圆环病毒2型PCR检测方法的建立及应用

›› 2010, Vol. 37 ›› Issue (8): 66-68.

• 生物技术 • Previous Articles Next Articles

Establishment and Application of PCR Detection Porcine Circovirus Type 2

ZHENG Yu-shu^1，2，ZHAO Pu¹，LIU Jun-wei¹，CHEN Jin-shan¹，YAO Si-xin¹，ZHAO Kun¹，LI Ren-feng¹，WANG San-hu^1，2，LIU Xing-you^1，2，ZHANG Zhi-yong¹

(1.Department of Animal Science and Technology，Henan Institute of Science and Technology, Xinxiang 453003, China;2.Henan Engineering Research Center of Animal Disease and Residue Prevention and Control,xinxiang 453003,China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-08-20 Published:2010-08-20

Abstract

Abstract: According to the conserved sequence of PCV2 published in GenBank, a pair of primers for amplification of PCV2 specified fragment was designed and a PCR assay for PCV2 detection was established. The results showed that the PCR was sensitive and specific, and useful to detect the clinic samples. It was helpful for clinical diagnosis and epidemiologic survey of PCV2.

Key words: porcine circovirus type 2; PCR; clinical diagnosis; epidemiologic survey

CLC Number:

S852.65⁺9.2

ZHENG Yu-shu;ZHAO Pu;LIU Jun-wei;CHEN Jin-shan;YAO Si-xin;ZHAO Kun;LI Ren-feng;WANG San-hu;LIU Xing-you;ZHANG Zhi-yong. Establishment and Application of PCR Detection Porcine Circovirus Type 2[J]. , 2010, 37(8): 66-68.

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Establishment and Application of PCR Detection Porcine Circovirus Type 2

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