Abstract: This experiment was research on produced results of layers by single and composite probiotics preparations. Bacillus Subtilis preparations, Lactobacillus preparations and composite preparations were prepared ferment culture by Bacillus Subtilis, Lactobacillus and composite probiotics. Then these preparations were added 0.1% based on basis grain to 140 days of ageRoman brown commercial layers. The results showed that probiotics preparations improved arerage egg weight, day egg production, laying rate and decreased feed/egg ratio. composite probiotics preparations improved average egg weight by 1.87%, 1.91%, day egg production by 3.34%,2.77%, laying rate by 1.56%, 1.00%, A level egg by 9.16%, 5.91%, vitelline rate by 7.71%, 4.14%,each layer gross profit by 10.84%, 7.73%;decreased feed/egg ratio 1.99%, 1.49% respectively,while in the decreased unqualified egg ratio,death rate,average daily feed consumption,egg shape index and increased egg propotion,eggshell thickness,there was no significant difference. This experiment showed that composite probiotics preparations effect was better than single probiotics preparations.

Key words: Lactobacillus; Bacillus Subtilis; probiotics; layer; produce effect